Antifibrotic effect of AD-1 on lipopolysaccharide-mediated fibroblast injury in L929 cells and bleomycin-induced pulmonary fibrosis in mice

Li, Tao; Chen, Yu; Li, Yuan; Chen, Gang; Zhao, Yuqing; Su, Guangyue

doi:10.1039/d1fo04212b

Cited by 6 publications

(5 citation statements)

References 63 publications

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“…Mouse fibroblast L929 cell line, possesses characteristics similar to lung fibroblasts in terms of hyperproliferation and activation, and can be induced into myofibroblasts with TGF-β1. TGF-β1 induced L929 cells are commonly used as the cell models of lung fibrosis ( Zhang T. et al, 2020 ; Li T. et al, 2022 ; Wang et al, 2024 ). The cytotoxic effects of MR and RA were evaluated by conducting CCK-8 assays on L929 cells exposed to different concentrations (0, 10, 20, 40, 80 and 160 μM) of MR and RA ( Figure 2A ).…”

Section: Resultsmentioning

confidence: 99%

Anti-pulmonary fibrosis activity analysis of methyl rosmarinate obtained from Salvia castanea Diels f. tomentosa Stib. using a scalable process

Ma,

Liu,

Zhao

et al. 2024

Front. Pharmacol.

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Pulmonary fibrosis is a progressive, irreversible, chronic interstitial lung disease associated with high morbidity and mortality rates. Current clinical drugs, while effective, do not reverse or cure pulmonary fibrosis and have major side effects, there are urgent needs to develop new anti-pulmonary fibrosis medicine, and corresponding industrially scalable process as well. Salvia castanea Diels f. tomentosa Stib., a unique herb in Nyingchi, Xizang, China, is a variant of S. castanea. and its main active ingredient is rosmarinic acid (RA), which can be used to prepare methyl rosmarinate (MR) with greater drug potential. This study presented an industrially scalable process for the preparation of MR, which includes steps such as polyamide resin chromatography, crystallization and esterification, using S. castanea Diels f. tomentosa Stib. as the starting material and the structure of the product was verified by NMR technology. The anti-pulmonary fibrosis effects of MR were further investigated in vivo and in vitro. Results showed that this process can easily obtain high-purity RA and MR, and MR attenuated bleomycin-induced pulmonary fibrosis in mice. In vitro, MR could effectively inhibit TGF-β1-induced proliferation and migration of mouse fibroblasts L929 cells, promote cell apoptosis, and decrease extracellular matrix accumulation thereby suppressing progressive pulmonary fibrosis. The anti-fibrosis effect of MR was stronger than that of the prodrug RA. Further study confirmed that MR could retard pulmonary fibrosis by down-regulating the phosphorylation of the TGF-β1/Smad and MAPK signaling pathways. These results suggest that MR has potential therapeutic implications for pulmonary fibrosis, and the establishment of this scalable preparation technology ensures the development of MR as a new anti-pulmonary fibrosis medicine.

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Section: Resultsmentioning

confidence: 99%

Anti-pulmonary fibrosis activity analysis of methyl rosmarinate obtained from Salvia castanea Diels f. tomentosa Stib. using a scalable process

Ma,

Liu,

Zhao

et al. 2024

Front. Pharmacol.

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“…MMPs are a family of enzymes that degrade collagen, and TIMPs speci cally inhibit MMPs activity. TIMP-1 not only inhibits MMPs activity and zymogen activation, but also promotes cell growth factor-like effect, which in turn promotes broblast proliferation and collagen synthesis [23]. Western blot results showed that the expressions of bronectin, collagen I, and α-SMA in the BLM group were signi cantly higher than those in the control group, and the expressions of these proteins were effectively inhibited after SIN treatment (Fig.…”

Section: Sin Alleviates Blm-induced Pf As Well As Neutralizes Ecm Dep...mentioning

confidence: 88%

Sinomenine Attenuates Pulmonary Fibrosis by Downregulating Tgf-Β1/Smad3, Pi3k/Akt and Nf-Κb Signaling Pathways

Yao,

Xu,

Dong

et al. 2023

Preprint

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“…188 Otherwise, a recent study showed that AD-1 alleviated lung fibrosis in vitro and in vivo by reducing extracellular matrix (ECM) accumulation, increasing the expression of the antioxidant enzymes SOD and GSH and regulating the TGF-β1/TIMP-1/α-SMA signalling pathway. 191…”

Section: Effects Of Ginsenosides On Pulmonary Inflammatory Diseasesmentioning

confidence: 99%

Ginsenosides: a potential natural medicine to protect the lungs from lung cancer and inflammatory lung disease

Wang,

Zhang,

Song

et al. 2023

Food Funct.

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Antifibrotic effect of AD-1 on lipopolysaccharide-mediated fibroblast injury in L929 cells and bleomycin-induced pulmonary fibrosis in mice

Abstract: 20(R)-25-methoxyl-dammarane-3 β, 12 β, 20-triol (25-OCH3-PPD, AD-1) is a dammarane ginsenoside that is isolated from Panax notoginseng. The present study aimed to explore its anti-pulmonary fibrosis (PF) effect in vitro...

Cited by 6 publications

References 63 publications

Anti-pulmonary fibrosis activity analysis of methyl rosmarinate obtained from Salvia castanea Diels f. tomentosa Stib. using a scalable process

Anti-pulmonary fibrosis activity analysis of methyl rosmarinate obtained from Salvia castanea Diels f. tomentosa Stib. using a scalable process

Sinomenine Attenuates Pulmonary Fibrosis by Downregulating Tgf-Β1/Smad3, Pi3k/Akt and Nf-Κb Signaling Pathways

Ginsenosides: a potential natural medicine to protect the lungs from lung cancer and inflammatory lung disease

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