Antifatigue effects of ethanol extracts and polysaccharides isolated from Abelmoschus esculentus

Abstract

Background:The aim of this study is to determine the antifatigue active fraction from Abelmoschus esculentus. The in vivo antifatigue effects of ethanol extracts and polysaccharides from A. esculentus fruit have been determined. The polysaccharides of A. esculentus were determined as the best effective fractions of antifatigue effects.Materials and Methods:About 360 Kunming male mice were randomly divided into nine subgroups: normal control subgroup, model subgroup, positive subgroup and the ethanol extracts of A. esculentus with high dose (3.2 g/kg) subgroup, medium dose (1.6 g/kg) subgroup and low dose (0.8 g/kg) subgroup, the polysaccharides of high dose (3.2 g/kg) subgroup, medium dose (1.6 g/kg) subgroup, and the low dose (0.8 g/kg) subgroup. The antifatigue effects of ethanol extracts and polysaccharides form A. esculentus were measured by comparing body weight, food intake, swimming time, liver glycogen, serum urea, blood lactic acid as well as visceral parameter in mice.Results:Compared with the model subgroup, other subgroups significantly prolonged swimming time, and high dose polysaccharides administration was the most effective (P < 0.01). High dose polysaccharides significantly increased liver glycogen, serum lactic acid, and serum urea (P < 0.01) in mice. In contrast with model group, the high dose polysaccharides administration could also significantly elevated the parameters of testicles and epididymis (P < 0.01). The study established that the ethanol extracts and polysaccharides of A. esculentus both have antifatigue effects.Conclusions:The results demonstrated that both the ethanol extracts and polysaccharides of A. esculentus have antifatigue effects. The high dosage polysaccharides have significant antifatigue properties. The results will provide the basis for further development and utilization of this plant.SUMMARY The high dosage polysaccharides have restoration ability on kidney yang deficiency mice.The high dosage polysaccharides have significant effects of relieving body fatigue of mice.The polysaccharide of Abelmoschus esculentus showed better antifatigue effects than the ethanol extracts. Abbreviations used: A. esculentus: Abelmoschus esculentus, BUN: Blood urine nitrogen, LD: Lactic Acid dehydrogenase, AE: Abelmoschus esculentus ethanol extracts, AP: Abelmoschus esculentus polysaccharides, LAC: Lactic acid content.

Carboxymethylated Sulfated Heteroexopolysaccharide from a Haloarchaeal Strain as Potential Biomolecule for Harmless Adjuvant Therapy in Cancer Treatment

“…Previous studies have attempted to identify antifatigue polysaccharides to delay fatigue and accelerate the elimination of tiredness. CS-hEPS showed better effects of relieving fatigue (64.62%) than Abelmoschus esculentus EPS (30.5%) [53]. ese effects are similar to Gynostemma pentaphyllum polysaccharide (GPP) which significantly prolonged exhaustive exercise time of mice.…”

“…In addition oral treatment with CS-hEPS increased the exercise tolerance, boosted the HG level by activating the energy metabolism, and reduced the accumulation of SUN and LDH contents during exercise, which could prolong the exercise durability. Due to the particularities of its chemical structure, CS-hEPS showed better antifatigue effects than those of previously reported polysaccharides [33,53,54,58]. e antitumor activity of CS-hEPS has also been tested against two types of cancer cell lines, myelogenous leukemia K562 and liver cancer Hep G cells, at different incubation periods and doses using 5-fluorouracil molecule (5-FU) as a positive control.…”

Understanding the traditional values and use of okra among pregnant women in western Ethiopia: a qualitative study

“…The ethanol extracts and polysaccharides of okra have antifatigue effects. 53 In line with this, okra seeds were the antifatigue part of okra pods; this is caused by reducing the levels of blood lactic acid and urea nitrogen and enhancing hepatic glycogen storage. 54 This study found that the consumption of okra makes pregnant women healthy, and is used to increase their life expectancy.…”

Vegetables from local markets in Myanmar

“…Flowers during April-May. Pharmacological properties and functions: Anti-fatigue (Li et al 2016); antidiabetic, and antihyperlipidemic activities (Sabitha et al 2011).…”