Antidiuretic hormone acts via V1 receptors on intracellular calcium in the isolated perfused rabbit cortical thick ascending limb

Nitschke, Roland; Fröbe, U.; Greger, R.

doi:10.1007/bf00372961

Cited by 114 publications

(55 citation statements)

References 53 publications

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“…Measurements of cytosolic calcium with the Ca 2ϩ -sensitive dye fura 2 (Sigma-Aldrich) were performed in podocytes on an inverted fluorescence microscope setup (13). The system allows fluorescence measurements at the single-cell level at three excitation wavelengths.…”

Section: Measurements Of the Free Cytosolic Intracellular Camentioning

confidence: 99%

Expression of Functional CCR and CXCR Chemokine Receptors in Podocytes

Huber

Reinhardt

Exner³

et al. 2002

The Journal of Immunology

108

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Chemokines and their receptors play an important role in the pathogenesis of acute and chronic glomerular inflammation. However, their expression pattern and function in glomerular podocytes, the primary target cells in a variety of glomerulopathies, have not been investigated as of yet. Using RT-PCR, we now demonstrate the expression of CCR4, CCR8, CCR9, CCR10, CXCR1, CXCR3, CXCR4, and CXCR5 in cultured human podocytes. Stimulation of these receptors induced a concentration-dependent biphasic increase of the free cytosolic calcium concentration in podocytes in culture. In addition, we demonstrate that podocytes release IL-8 in the presence of FCS and that IL-8 down-regulates cell surface CXCR1. Chemokine stimulation of the detected CCRs and CXCRs increased activity of NADPH-oxidase, the primary source of superoxide anions in podocytes. Immunohistochemistry studies revealed only diffuse and weak CXCR expression in healthy human glomerula. In contrast, in membranous nephropathy, a characteristic podocyte disorder, the expression of CXCR1, CXCR3, and CXCR5 is up-regulated in podocytes. In conclusion, podocytes in culture and podocytes in human kidney sections express a set of chemokine receptors. The release of oxygen radicals that accompanies the activation of CCRs and CXCRs may contribute to podocyte injury and the development of proteinuria during membranous nephropathy.

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Section: Measurements Of the Free Cytosolic Intracellular Camentioning

confidence: 99%

Expression of Functional CCR and CXCR Chemokine Receptors in Podocytes

Huber

Reinhardt

Exner³

et al. 2002

The Journal of Immunology

108

View full text Add to dashboard Cite

show abstract

“…The distribution of Vla and V2 receptors in the kidney has been investigated by binding studies (7-1 1 ) and many physiological studies (12)(13)(14)(15)(16)(17). The presence of V2 receptor in the collecting duct is well established (9,11,18).…”

Section: Introductionmentioning

confidence: 99%

Different localization and regulation of two types of vasopressin receptor messenger RNA in microdissected rat nephron segments using reverse transcription polymerase chain reaction.

Terada¹,

Tomita²,

Nonoguchi³

et al. 1993

J. Clin. Invest.

124

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Recent studies have revealed that arginine vasopressin (AVP) has at least two types of receptors in the kidney: Vla receptor and V2 receptor. In this study, microlocalization of mRNA coding for Vla and V2 receptors was carried out in the rat kidney using a reverse transcription and polymerase chain reaction. Large signals for Vla receptor PCR product were detected in the glomerulus, initial cortical collecting duct, cortical collecting duct, outer medullary collecting duct, inner medullary collecting duct, and arcuate artery. Small but detectable signals were found in proximal convoluted and straight tubules, inner medullary thin limbs, and medullary thick ascending limbs. Large signals for V2 receptor mRNA were detected in the cortical collecting duct, outer medullary collecting duct, and inner medullary collecting duct. Small signals for V2 receptor were found in the inner medullary thick limbs, medullary thick ascending limbs, and initial cortical collecting duct. Next, we investigated Vla and V2 receptor mRNA regulation in the dehydrated state. During a 72-h water restriction state, the plasma AVP level increased and V2 receptor mRNA decreased in collecting ducts. In contrast, Vla receptor mRNA did not change significantly. Thus, the two AVP receptor subtypes are distributed differently along the nephron, and these mRNAs are regulated differently in the dehydrated state. (J. Clin. Invest.

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“…The distribution of V1a and V2 receptors in the kidney has been well studied in rat and rabbit by radioligand binding studies (18,20,48,57) and several physiological functional assays (17,27,29,31,44). Intrarenal localization of V1a remains less well defined.…”

mentioning

confidence: 99%

Axial heterogeneity of vasopressin-receptor subtypes along the human and mouse collecting duct

Carmosino¹,

Brooks²,

Cai³

et al. 2007

American Journal of Physiology-Renal Physiology

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Vasopressin and vasopressin antagonists are finding expanded use in mouse models of disease and in clinical medicine. To provide further insight into the physiological role of V1a and V2 vasopressin receptors in the human and mouse kidney, intrarenal localization of the receptors mRNA was determined by in situ hybridization. V2-receptor mRNA was predominantly expressed in the medulla, whereas mRNA for V1a receptors predominated in the cortex. The segmental localization of vasopressinreceptor mRNAs was determined using simultaneous in situ hybridization and immunohistochemistry for segment-specific markers, including aquaporin-2, Dolichos biflorus agglutinin, epithelial Na channels, Tamm Horsfall glycoprotein, and thiazide-sensitive Na ϩ -Cl Ϫ cotransporter. Notably, V1a receptor expression was exclusively expressed in V-ATPase/anion exchanger-1-labeled alpha-intercalated cells of the medullary collecting duct in both mouse and human kidney. In cortical collecting ducts, V1a mRNA was more widespread and detected in both principal and intercalated cells. V2-receptor mRNA is diffusely expressed along the collecting ducts in both mouse and human kidney, with higher expression levels in the medulla. These results demonstrate heterogenous axial expression of both V1a and V2 vasopressin receptors along the human and mouse collecting duct. The restricted expression of V1a-receptor mRNA in intercalated cells suggests a role for this receptor in acid-base balance. These findings further suggest distinct regulation of renal transport function by AVP through V1a and V2 receptors in the cortex vs. the medulla. in situ hybridization; vasopressin receptors; intercalated cell ARGININE VASOPRESSIN (AVP) is the key hormone responsible for producing a concentrated urine and is secreted in high concentrations during antidiuresis. At least two different vasopressin

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Antidiuretic hormone acts via V1 receptors on intracellular calcium in the isolated perfused rabbit cortical thick ascending limb

Cited by 114 publications

References 53 publications

Expression of Functional CCR and CXCR Chemokine Receptors in Podocytes

Expression of Functional CCR and CXCR Chemokine Receptors in Podocytes

Different localization and regulation of two types of vasopressin receptor messenger RNA in microdissected rat nephron segments using reverse transcription polymerase chain reaction.

Axial heterogeneity of vasopressin-receptor subtypes along the human and mouse collecting duct

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