Antidiarrheal effects of water-soluble proteins from Plumeria pudica latex in mice

Santana, Lucas de Araújo Bastos; Aragão, Diêgo Passos; Araújo, Thiago de Souza Lopes; Sousa, Nayara Alves de; Souza, Luan Kelves Miranda de; Oliveira, Lucas Eduardo Silva; Pereira, Anna Carolina Toledo da Cunha; Ferreira, Gustavo Portela; Oliveira, Naylla Veras de Moraes; Souza, Bruna da Silva; Sousa, Francisca Beatriz M.; Ramos, Márcio V.; Freitas, Cléverson D.T.; Medeiros, Jand Venes R.; Oliveira, Jefferson Soares de

doi:10.1016/j.biopha.2017.11.019

Cited by 11 publications

(9 citation statements)

References 35 publications

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“…Figure 6a,b shows that intraluminal injection of RAG (60 mg/kg) decreased CT-induced intestinal fluid secretion by 0.095 ± 0.008 g/cm for the CT-treated group versus 0.046 ± 0.005 g/cm for the RAG treated group (P < 0.05) after 4 hours. These early results show that RAG is able to reduce fluid production after four hours of cholera toxin-induced diarrhea, as reported by [57], [73], and [74], proving the action of different types of natural products against cholera toxin-induced diarrhea.…”

Section: Ex Vivo Antispasmodic Activitysupporting

confidence: 72%

Biopolymer Extracted from Anadenanthera colubrina (Red Angico Gum) Exerts Therapeutic Potential in Mice: Antidiarrheal Activity and Safety Assessment

Araújo

Oliveira²,

Sousa

et al. 2020

Pharmaceuticals

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Anadenanthera colubrina var. cebil (Griseb.) Altschul (Fabaceae family), commonly known as the red angico tree, is a medicinal plant found throughout Brazil’s semi-arid area. In this study, a chemical analysis was performed to investigate the antidiarrheal activity and safety profile of red angico gum (RAG), a biopolymer extracted from the trunk exudate of A. colubrina. Upon FT-IR spectroscopy, RAG showed bands in the regions of 1608 cm−1, 1368 cm−1, and 1029 cm−1, which relate to the vibration of O–H water molecules, deformation vibration of C-O bands, and vibration of the polysaccharide C-O band, respectively, all of which are relevant to glycosidic bonds. The peak molar mass of RAG was 1.89 × 105 g/mol, with the zeta potential indicating electronegativity. RAG demonstrated high yield and solubility with a low degree of impurity. Pre-treatment with RAG reduced the total diarrheal stool and enteropooling. RAG also enhanced Na+/K+-ATPase activity and reduced gastrointestinal transit, and thereby inhibited intestinal smooth muscle contractions. Enzyme-Linked Immunosorbent Assay (ELISA) demonstrated that RAG can interact with GM1 receptors and can also reduce E. coli-induced diarrhea in vivo. Moreover, RAG did not induce any signs of toxicity in mice. These results suggest that RAG is a possible candidate for the treatment of diarrheal diseases.

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Section: Ex Vivo Antispasmodic Activitysupporting

confidence: 72%

Biopolymer Extracted from Anadenanthera colubrina (Red Angico Gum) Exerts Therapeutic Potential in Mice: Antidiarrheal Activity and Safety Assessment

Araújo

Oliveira²,

Sousa

et al. 2020

Pharmaceuticals

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“…Our research team is investigating this protein fraction in the last years. Besides mentioned anti-inflammatory activity, we investigated its antidiarrheal properties (Santana et al;, its protective effect on ulcerative colitis (Oliveira et al, 2019) and its effects and periodontitis (Oliveira et al,2021) also against induced gastritis injury by ethanol (data not published). These interesting The toxic effects of other latexes have been previously reported.…”

Section: Discussionmentioning

confidence: 99%

Acute and Subchronic Toxicity Assessment of Proteins from Plumeria pudica Latex in Mice

Souza¹,

Oliveira²,

Moita³

et al. 2021

RSD

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Latex proteins extracted from Plumeria pudica (LPPp) have anti-inflammatory activity in different experimental models. Considering this relevant result, we evaluated the toxicological aspects of the treatment of mice with LPPp. Acute and subchronic toxicities were determined by daily intraperitoneal administration of 40 mg/kg of LPPp for 10 or 20 days, respectively, and were followed by behavioral, hematological, biochemical and histopathological evaluation. Results showed no significant changes in body weight and organs of animals treated with LPPp. Total and differential blood leukocyte counts of LPPp groups did not differ from controls. There was no significant difference in aspartate aminotransferase, alanine aminotransferase, creatinine and urea measurements for the group treated for 20 days. The level of glutathione in the kidney was significantly higher in animals treated with LPPp in the acute toxicity test, but no differences were observed for the subchronic evaluation. The concentration of malondialdehyde and myeloperoxidase activity in the organs did not differ from controls. Histopathological examination of kidney, spleen and liver tissue of animals treated with LPPp revealed normal structures or reversible alterations. Intraperitoneal LD50 of LPPp was higher than 2,000 mg/kg. The data obtained reveal that LPPp is nontoxic at 40 mg/kg.

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“…Vel azquez et al [131] Protein fraction Plumeria pudica latex Santana et al [129] Berberine (alkaloid)…”

Section: Activators Of Adp-ribosylarginine Hydrolasementioning

confidence: 99%

“…[128] Another natural product extracted from plants that also presented an antidiarrhoeal effect in different diarrhoeal trials including that induced by CT was a protein fraction obtained from Plumeria pudica latex. [129] Bag et al [130] showed the effect of the inhibitor of methyl gallate, a phenolic compound extracted from the Terminalia chebula plant, on V. cholerae colonization and inhibition of fluid accumulation, as well as the inflammatory response induced by V. cholerae in vivo.…”

Section: Medicinal Plants As Sources Of Promising Compoundsmentioning

confidence: 99%

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A comprehensive review of therapeutic approaches available for the treatment of cholera

Sousa

Nolêto

Chaves

et al. 2020

Journal of Pharmacy and Pharmacology

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Objectives The oral rehydration solution is the most efficient method to treat cholera; however, it does not interfere in the action mechanism of the main virulence factor produced by Vibrio cholerae, the cholera toxin (CT), and this disease still stands out as a problem for human health worldwide. This review aimed to describe therapeutic alternatives available in the literature, especially those related to the search for molecules acting upon the physiopathology of cholera. Key findings New molecules have offered a protection effect against diarrhoea induced by CT or even by infection from V. cholerae. The receptor regulator cystic fibrosis channel transmembrane (CFTR), monosialoganglioside (GM1), enkephalinase, AMP-activated protein kinase (AMPK), inhibitors of expression of virulence factors and activators of ADP-ribosylarginine hydrolase are the main therapeutic targets studied. Many of these molecules or extracts still present unclear action mechanisms. Conclusions Knowing therapeutic alternatives and their molecular mechanisms for the treatment of cholera could guide us to develop a new drug that could be used in combination with the rehydration solution.

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Antidiarrheal effects of water-soluble proteins from Plumeria pudica latex in mice

Cited by 11 publications

References 35 publications

Biopolymer Extracted from Anadenanthera colubrina (Red Angico Gum) Exerts Therapeutic Potential in Mice: Antidiarrheal Activity and Safety Assessment

Biopolymer Extracted from Anadenanthera colubrina (Red Angico Gum) Exerts Therapeutic Potential in Mice: Antidiarrheal Activity and Safety Assessment

Acute and Subchronic Toxicity Assessment of Proteins from Plumeria pudica Latex in Mice

A comprehensive review of therapeutic approaches available for the treatment of cholera

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