Antidiarrheal Effect of Fermented Millet Bran on Diarrhea Induced by Senna Leaf in Mice

Abstract

Bacillus natto is a kind of probiotic with various functional characteristics, which can produce a lot of nutrients during growth and reproduction. Bacillus natto was used as strain, the number of viable bacteria and the content of soluble dietary fiber in millet bran were used as indexes to study the effects of inoculum size, fermentation time, and fermentation temperature on the fermentation effect, and the optimal fermentation conditions were determined by a response surface experiment. The antidiarrhea effect of fermented millet bran prepared under the best technological conditions was evaluated. The results showed that the optimum fermentation conditions were as follows: inoculum size was 7.48%, fermentation time was 47.04 h, and fermentation temperature was 36.06 °C. Under the optimal fermentation conditions, the viable bacteria count of millet bran was 8.03 log CFU/mL and the soluble dietary fiber content was 12.14%. The fermented millet bran can significantly reduce the intestinal thrust rate and serum levels of IL-6, IL-12, and TNF-α, and significantly increase the secretion of SIgA in the intestinal mucosa, which can relieve diarrhea induced by senna leaf in mice. The results of this study can provide the scientific basis for deep processing of millet bran and efficient utilization of fermented millet bran, and also provide the theoretical basis for clinical treatment of diarrhea.

Dysfunction of cecal microbiota and CutC activity in mice mediating diarrhea with kidney-yang deficiency syndrome

“…TMAO is involved in the development of cardiovascular diseases and chronic kidney diseases by upregulating the expression of inflammatory factors such as TNF-α and IL-6 ( Al-Obaide et al, 2017 ; Canyelles et al, 2023 ). The up-regulated expression of the inflammatory factors TNF-α and IL-6 increases the permeability of vascular endothelial cells, leading to intestinal fluid extravasation, which can cause diarrhea ( Chen et al, 2022 ). It can be speculated that TMAO may be involved in the occurrence and development of diarrhea by promoting inflammatory reactions.…”

Functional Ingredients in Minor Grain Crops

“…This provides a new strategy for the extraction of ELA and shows the potential application of millet bran in health food. Furthermore, fermentation technology was utilized to increase the soluble dietary fiber content in millet bran by Chen et al [ 5 ]. They found that the intestinal thrust rate and serum levels of immune factors were down-regulated, while the secretion of secretory immunoglobulin A in the intestinal mucosa was up-regulated by millet bran fermented with Bacillus natto .…”