Antidiarrheal activity of 80 % methanol extract of the aerial part of Ajuga remota Benth (Lamiaceae) in mice

Abstract

BackgroundIn the Ethiopian traditional medicine, the aerial part of Ajuga remota Benth is used in the treatment of diarrhea. There are different mechanisms by which Ajuga remota may have antidiarrheal effect. Some of the possible mechanisms are through its anthelmintic and antibacterial activity. The present study aimed to evaluate whether the antidiarrheal effect of the plant also include antimotility and antisecretory effect using 80 % methanol extract of A. remota (MEAR).MethodsThe MEAR was administered at doses of 200, 400, 600 and 800 mg/kg to four groups of mice (six animals per group) orally in castor oil diarrhea model. The effect of the extract on enteropooling and gastrointestinal transit model was also evaluated using the same grouping and dosing. Two other groups, one as control and the other as standard (loperamide 5 mg/kg) were used for comparison with the treatment groups.ResultsThe extract at the doses of 400, 600, and 800 mg/kg produced a dose-dependent and significant inhibition both on the frequency and onset of diarrhea. The percentage purging frequency was 53.4, 66.7, 79.6, and 66.7 % (p < 0.001) at three doses of MEAR (400, 600, and 800 mg/kg) and with loperamide (5 mg/kg), respectively. The percentage inhibition in intestinal fluid accumulation was 42.5, 62.1, and 74.2 % (p < 0.001) at the doses of 400, 600 and 800 mg/kg of MEAR, respectively. The MEAR also inhibited the intestinal transit of charcoal meal in a dose dependent manner both in the normal and castor oil induced intestinal transit.ConclusionThis study has shown that the 80 % methanol extract of A. remota contains pharmacologically active substances with significant antimotility and antisecretory effect contributing for its antidiarrheal activity.

Evaluation of in-vivo antidiarrhoeal and in-vitro antibacterial activities of the root extract of Brucea antidysenterica J. F. Mill (Simaroubaceae)

“…This might be associated with the smallest dose of test extract has not sufficient ability to prolong the onset of diarrhoea. As explained by other reports doses having lower antimotility and/or antisecretory effects are less likely to address all the parameters [10,12,15].…”

Evaluation of the anti-diarrheal activity of the aqueous stem extract of Lantana camara Linn (Verbenaceae) in mice

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Investigating Potential Modes of Actions of Mimusops kummel Fruit Extract and Solvent Fractions for Their Antidiarrheal Activities in Mice

“…It was then expressed according to the following formula [29]:$\begin{array}{c}T3\mathrm{I}\mathrm{n}\hspace{0.17em}\hspace{0.17em}\mathrm{v}\mathrm{i}\mathrm{v}\mathrm{o}\hspace{0.17em}\hspace{0.17em}\mathrm{a}\mathrm{n}\mathrm{t}\mathrm{i}\mathrm{d}\mathrm{i}\mathrm{a}\mathrm{r}\mathrm{r}\mathrm{h}\mathrm{e}\mathrm{a}\mathrm{l}\hspace{0.17em}\hspace{0.17em}\mathrm{i}\mathrm{n}\mathrm{d}\mathrm{e}\mathrm{x}\hspace{0.17em}\hspace{0.17em}\left(\mathrm{A}\mathrm{D}\mathrm{I}\right)\\ =\sqrt[\mathrm{3}]{\mathrm{D}\mathrm{f}\mathrm{r}\mathrm{e}\mathrm{q}\times \mathrm{G}\mathrm{m}\mathrm{e}\mathrm{q}\times \mathrm{P}\mathrm{f}\mathrm{r}\mathrm{e}\mathrm{q}},\end{array}$where Dfreq is the delay in defecation time as percentage of negative control, Gmeq is the gut meal travel reduction as percentage of negative control, and Pfreq is the reduction in purging frequency in the number of wet stools as percentage of negative control.…”