Antidiabetic Effect of Methanolic Extract fromBerberis julianaeSchneid. via Activation of AMP-Activated Protein Kinase in Type 2 Diabetic Mice

Abstract

We have investigated the antidiabetic effect and mechanism of methanolic extract of Berberis julianae Schneid. (BJSME) in STZ induced Type 2 diabetes mellitus mice. T2DM mice were induced by high fat diet and low dose streptozotocin (STZ). BJSME was orally administrated at the doses of 60, 120, and 240 mg/kg/d, for 21 days. Metformin was used as positive control drug. Food intake, body weight, plasma glucose, oral glucose tolerance test, insulin tolerance test, insulin, and blood-lipid content were measured. The effects of BJSME on the glucose transporter 4 (GLUT4) translocation in L6 myotubes and the GLUT4 protein expression in skeletal muscle as well as phosphorylation of the AMP-activated protein kinase (AMPK) in liver and muscle were examined. In vitro and in vivo results indicate that BJSME increased GLUT4 translocation by 1.8-fold and BJSME significantly improved the oral glucose tolerance and low density lipoprotein cholesterol (LDL-C) of serum and reduced body weight, glucose, and other related blood-lipid contents. The BJSME treatment also stimulated the phosphorylation of AMPK. Thus, BJSME seems to possess promising beneficial effects for the treatment of T2DM with the possible mechanism via stimulating AMPK activity.

Antidiabetic Activity of Ergosterol from Pleurotus Ostreatus in KK‐Ay Mice with Spontaneous Type 2 Diabetes Mellitus

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Antidiabetic effects of flavonoids from Sophora flavescens EtOAc extract in type 2 diabetic KK-ay mice

“…Therefore, the search for more effective and safer natural hypoglycemic agents has continued to be an important area of investigation. The traditional Chinese medicines (TCMs) have been used to counteract DM (recognized as "Xiao Ke Zheng" in ancient China) for thousand years based on unique theory system with few side effects, and it has been attracting more and more attention for its dialectical therapy Yang et al, 2014).…”

Hypoglycemic effect of <i>Berberis microphylla</i> G Forst root extract

“…With respect to possible mechanisms of action, B. julianae extract has been shown to increase translocation and expression of glucose transporter GLUT4 in muscle cells L6, causing increased glucose uptake as well as increasing the phosphorylation of AMPK in the hepatic and muscular tissue of mice [21]. In the same manner, it has been demonstrated in mouse muscle cells that berberine is involved in the activation of AMPK and p38 MAPK [22].…”