Antidiabetic and antioxidant effects of Lupinus albus L. seed roasting ethanol extract in streptozotocin diabetic rats

Abstract

Diabetes mellitus (DM) may cause specially health problem which can effect most of tissue such as liver, kidney and brain. Thus, this research aimed to examining the effects of Lupinus albus L. seed ethanol extract (LA) on the liver and renal tissues malondialdehyde (MDA), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione (GSH) level of diabetic rats. Sprague-Dawley, adult a total of 28 male rats were used in the study that were randomly divided into four groups with 7 animals in each group: Control, STZ-DM, LA and STZ-DM+LA group. Diabetes was induced in rats by a single dose of 60 mg/kg/i.p. Streptozotocin (STZ) injection (STZ-DM and STZ-DM+LA groups). Lupinus albus L. ethanol extract was given at a dose of 10 mg/kg/p.o. via intragastric tube for 20 days (LA and STZ-DM+LA groups). At the end of the study, biochemical and histopathological examination in kidney and liver tissues were examined. STZ application significantly increased the oxidative stress and lipid peroxidation. When the dose of 10 mg/kg of LA significantly decreased the level of plasma glucose and liver and renal MDA. Meanwhile, the levels of liver and renal CAT, SOD, GPx and GSH were significantly increased in diabetic rats treated with Lupinus albus L. seed ethanol extract. These results indicate the role of Lupinus albus L. seed extract might as antidiabetic, antioxidant and reducing of the oxidative stress and lipid peroxidation. Extracted after Lupinus albus seed' roasting is a pioneer in literature and the use of LA in ethnomedicine for diabetes.