Antidiabetic and Antihyperlipidemic Effects of Methanolic Extract of Leaves of Spondias mombin in Streptozotocin-Induced Diabetic Rats

Abstract

Objective:Spondias mombin is a plant that reported to have anticonvulsant, antimicrobial, antioxidant, antiulcer, antiasthmatic, and wound healing activities. Diabetes dyslipidemic effect of Spondias mombin leaves is not clear. Hence, current study planned to evaluate the antidiabetic and antihyperlipidemic effects of methanolic extract of leaves of Spondias mombin (MESM) in streptozotocin (STZ) induced diabetic rats.Methods: Phytochemicals were determined by standard method and antioxidant activity was determined by DPPH free radical scavenging and FRAP assay. Diabetes was induced by injecting a single dose of STZ (55 mg/kg) into female sprague dawley rats. After 3 days of induction of diabetes, the diabetic animals were treated for 28 days with MESM (125, 250, and 500 mg/kg) and glibenclamide (20 mg/kg) orally. The body weight of rats and blood glucose levels were monitored at regular intervals during the experiment. At the end of study, blood sample was collected from all the animals and subjected to biochemical, lipid profile, and they were sacrificed and their organs such as pancreas, liver and kidney were used for histopathological analysis.Results: Quantitative analysis of MESM showed the presence of anthraquinone, tannins, saponins, steroid, phenols, flavonoids, alkaloids, and reducing sugars. Reduction in body weight and elevated blood glucose were observed in diabetic rats. Treatment with MESM in a concentration of 125, 250, and 500 mg/kg significantly reversed the elevated levels of blood glucose, reduced aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), total bilirubin, urea, creatinine, total serum cholesterol (TC), serum triglyceride (TG), low-density lipoprotein (LDL), Very low-density lipoprotein (VLDL), and increased plasma insulin, total protein, albumin, globulin, A/G ratio, and high-density lipoprotein (HDL).Conclusion: MESM exhibited a significant antidiabetic and antihyperlipidemic activities against STZ-induced diabetes in rats.

Antidiabetes study of Spondias mombin (Linn) stem bark fractions in high-sucrose diet-induced diabetes in Drosophila melanogaster

“…The anticancer potential of carotenoid isolates from the leaves of S. mombin has been documented by Metibemu et al. 26 Although previous studies have reported the antidiabetic activity of S. mombin leaves, 10 , 11 the antidiabetic efficacy of solvent fractions of S. mombin stem bark in the HSD-induced diabetes Drosophila model has not been explored.…”

“…9 The antidiabetic potential of S. mombin leaves has been reported in alloxan-induced rats 10 and streptozotocin-induced diabetic rats. 11 The hypoglycemic effect of S. mombin stem bark has been reported in healthy rats. 12 After establishing high-sucrose diet (HSD)-induced diabetes in Drosophila as in previous studies, 5 , 13 this study investigated the antidiabetic potential of ethyl acetate and n-butanol fractions of S. mombin stem bark in this HSD-induced Drosophila model.…”

Antidiabetic activity of Solanum torvum fruit extract in streptozotocin-induced diabetic rats

“…This could be due to the sufficient intake of glucose by the cells after the treatment with glibenclamide or S. torvum fruit extract. Most of the experimental studies suggested that increased gluconeogenesis is a main source of hyperglycemia in insulin deficiency ( 65 ). It is also suggested that insulin may inhibit PCK1 gene transcription by activation of a possible insulin response factors ( 66 ).…”

Novel Antidiabetic Polyherbal Formulation for Synergistic Therapeutic Effects in Streptozotocin (STZ)-Induced Diabetic Rats

“…Because glucose is used up more slowly in diabetics, a condition called diabetesinduced hyperlipidemia is to blame for the excessive release of fat from adipose tissue. 23 Treatment with a polyherbal formulation resulted in lower levels of cholesterol, triglyceride, and LDL, and higher levels of HDL, which is suggestive of the formulations' potential ability to lower blood lipid levels (hypolipidemic activity).…”

“…The long-term health effects of hyperglycemia include damage and eventual failure of several organs, most notably the nerves, blood vessels, kidneys, eyes, and heart. 1,2 Diabetes is currently becoming a serious concern on a global scale and a main cause of morbidity and death in the majority of countries. It is estimated that there are 382 million individuals who are affected by the disease, and 5.1 million people lost their lives to diabetes in 2013.…”