Antidiabetic Activity ofGnidia glaucaandDioscorea bulbifera: Potent Amylase and Glucosidase Inhibitors

Abstract

Diabetes is a metabolic disorder affecting about 220 million people worldwide. One of the most critical complications of diabetes is post-prandial hyper-glycemia (PPHG). Glucosidase inhibitor and α-amylase inhibitors are class of compounds that help in managing PPHG. Low-cost herbal treatment is recommended due to their lesser side effect for treatment of diabetes. Two plants with significant traditional therapeutic potential, namely, Gnidia glauca and Dioscorea bulbifera, were tested for their efficiency to inhibit α-amylase and α-glucosidase. Stem, leaf, and flower of G. glauca and bulb of D. bulbifera were sequentially extracted with petroleum ether, ethyl acetate, and methanol as well as separately with 70% ethanol. Petroleum ether extract of flower of G. glauca was found to inhibit α-amylase significantly (78.56%). Extracts were further tested against crude murine pancreatic, small intestinal, and liver glucosidase enzyme which revealed excellent inhibitory properties. α-glucosidase inhibition provided a strong in vitro evidence for confirmation of both G. glauca and D. bulbifera as excellent antidiabetic remedy. This is the first report of its kind that provides a strong biochemical basis for management of type II diabetes using G. glauca and D. bulbifera. These results provide intense rationale for further in vivo and clinical study.

Gnidia glauca Leaf and Stem Extract Mediated Synthesis of Gold Nanocatalysts with Free Radical Scavenging Potential

“…Equilateral nanotriangles observed might be due to the halide ions in the reaction mixture as mentioned earlier [43]. Similar results obtained by Kumar et al, where AuNPs were synthesized employing an antidiabetic plant, Cassia auriculata thus provides strong rationale for synthesis of AuNPs by G. glauca, which we have recently reported as a potent antidiabetic plant [29,41].…”

“…Leaves have been applied to treat the contusions, swellings, back ache and joint aches [28]. Similarly, recently we have reported its antidiabetic activity [29].…”

Antioxidant, anti-acetylcholinesterase and anti-glycosidase properties of three species of Swertia, their xanthones and amarogentin: A comparative study

“…α-Glucosidase inhibitor and α-amylase inhibitors help in managing post-prandial hyperglycemia 27 During the present study, it was observed that the three species of Swertia had very little α-amylase inhibitory activity (Table 3). But the methanolic extracts of all the three species inhibited α-glucosidase activity in a dose dependant manner.…”

Biochemical Evaluation of the Effects of Hydromethanolic Extracts of Dioscorea bulbifera in Wistar Rats

“…This is attributable to the effect of Dioscorea bulbifera which has been shown to be a potent potentglucosidase and α-amylase inhibitor [21] which lowers the rate of glucose absorption through delayed carbohydrate digestion . It may also be due to the an extra pancreatic mechanism of inhibition of hepatic glucose production [22,23].…”