Antidiabetic activity of hydro-alcoholic stem bark extract of Callicarpa arborea Roxb. with antioxidant potential in diabetic rats

Junejo, Julfikar Ali; Rudrapal, Mithun; Nainwal, Lalit Mohan; Zaman, Kamaruz

doi:10.1016/j.biopha.2017.08.032

Cited by 41 publications

(35 citation statements)

References 56 publications

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“…In our earlier studies, the antidiabetic activity of the methanolic bark extract of C. dichotoma has already been reported [4]. Moreover, literature suggest that the flavonoids content demonstrates antidiabetic efficacy of many plants [14][15][16][17][18]. Our docking study validates the antidiabetic claim about C. dichotoma reported in traditional medicines as well as in recent literature.…”

Section: Molecular Dockingsupporting

confidence: 86%

Bioactive Flavonoids from the Stem Bark of <em>Cordia dichotoma</em> Forst.: Identification, Docking and ADMET Studies

Hussain¹,

Bb²,

Rudrapal³

et al. 2021

Preprint

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Cordia dichotoma Forst. (F. Boraginaceae) has been traditionally used for the management of a variety of human ailments. In our earlier work, the antidiabetic activity of methanolic bark extract of C. dichotoma (MECD) has been reported. In this paper, two flavonoid molecules were isolated (by column chromatography) and identified (by IR, NMR and Mass spectroscopy/spectrometry) from the MECD with an aim to investigate their antidiabetic effectiveness. Molecular docking and ADMET studies were carried out using AutoDock Vina software and Swiss ADME online tool, respectively. The isolated flavonoids were identified as 3,5,7,3’,4’-tetrahydroxy-4-methoxyflavanone-3-O-L-rhamnopyranoside and 5,7,3’-trihydroxy-4-methoxyflavanone-7-O-L-rhamnopyranoside (quercitrin). Docking and ADMET studies revealed the promising binding affinity of flavonoid molecules for human lysosomal alpha-glucosidase and human pancreatic alpha-amylase with acceptable ADMET properties. Based on computational studies, our study reports the antidiabetic potential of the isolated flavonoids with predictive pharmacokinetics profile.

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Section: Molecular Dockingsupporting

confidence: 86%

Bioactive Flavonoids from the Stem Bark of <em>Cordia dichotoma</em> Forst.: Identification, Docking and ADMET Studies

Hussain¹,

Bb²,

Rudrapal³

et al. 2021

Preprint

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“…Diabetes induced by STZ causes weight loss in animals which is due to lack of insulin and proteinuria. Therefore the lack of insulin stimulate the liver to breakdown protein into amino acids resulting to excessive weight loss [27], which was observed in the study. Among two test doses (50 and 100mg/kg) P. macrocarpus at 100 mg/kg was found to be more effective in increasing the body weight compared to diabetic control rats.…”

Section: Discussionmentioning

confidence: 68%

Phytochemical Evaluation and Anti-Diabetic Effects of Ethanolic Leaf Extract of Petersianthus macrocarpus on Streptozotocin-Induced Diabetic Rats

Ugochukwu

Anna

2021

JAMMR

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Aim: The purpose of this study was to evaluate the antidiabetic effect of ethanolic extracts of Petersianthus macrocarpus leaf and its phytochemical analysis using different solvents. Place and Duration of Study: The study was carried out between March and September in 2018 in the Department of Anatomy, Madonna University, Elele. River State, Nigeria. Materials and Methods: Twenty five Wistar rats, weighing between 200-225g were divided into five groups of five rats. Group A (Control) while Groups B, C, D and E were induced with diabetes using streptozotocin firstly 35mg/kg b.w; and 2 weeks later 25mg/kg b.w. Group B (Diabetic control), Group C received 0.5mg/kg b.w of Glibenclamide. Groups D and E received daily 50 and 100 mg/kg b.w of ethanolic leaf extract of P. macrocarpus orally for two weeks. The fasting blood glucose levels were determined weekly for two weeks. At the end of the experiment, the animals were sacrificed and the pancreas was removed for histological procedures. Results: The body weights increased significantly (P<0.05) in 100 mg/kg b.w group when compared to the diabetic control rats weight at the end of the experiment. Also there was a significantly decreased (P<0.05) in blood glucose levels in P. macrocarpus (100mg/kg b.w). The histological section of the pancreas of diabetic control showed eosinophilic material in the islet, shrinkage of the islet of Langerhans while the group treated with 100 mg/kg of extract showed granulated and well prominent pancreatic islet of Langerhans. Phytochemical screening showed methanolic extract of P. macrocarpus leaf having alkaloid, saponin, tannin, phenol, flavonoid, cardiac glycoside, steroids, terpenoids, anthocyanin and anthraquinone. However, cardiac glycosides and steroids were absent in ethanolic extract. Cardiac glycoside and terpenoids were also absent in hexane and acetone extract, while phenol, cardiac glycosides, steroids and anthrocyanin were absent in the water extract. Conclusion: Ethanolic leaf extract of P. macrocarpus ameliorate streptozotocin-induced diabetes in Wistar rats.

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“…Oral glucose tolerance test is used to determine the altered carbohydrate metabolism during post glucose administration 13 . The three test doses (100, 200, 400 mg/kg) of LCME showed improvement in oral glucose tolerance.…”

Section: Discussionmentioning

confidence: 99%

“…ii) STZ-induced diabetes is known to cause weight loss in animals as a result of proteinuria and insulin deficiency. Insulin deficiency triggers the liver to breakdown protein into amino acids leading to muscle wasting and excessive weight loss 13 which was observed only after 21 days of the treatment. Among three test doses (100, 200, 400mg/kg) LCME at 200 mg/kg was found to be more effective in maintaining the body weight compared to diabetic control rats and reference drug glibenclamide treated rats, which may be due to their protective effect in controlling muscle wasting i.e.…”

Section: Discussionmentioning

confidence: 99%

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2020

IJPSR

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During the past few years many phytochemicals responsible for anti-diabetic effects have been isolated from the plants. Several phytoconstituents such as alkaloids, glycosides, flavonoids, saponins, dietary fibers, polysaccharides, glycolipids, peptidoglycans, amino acids and others obtained from various plant sources that have been reported as potent hypoglycemic agents. In the present study, the effect of methanolic extract of whole plant of Lindernia ciliata (LCME) (100, 200 and 400 mg/kg) on glucose levels in streptozotocin (STZ) (45 mg/kg) induced diabetic rats was investigated using glibenclamide 10 mg/kg as the positive control. The effect of the extract on body weight, insulin, total cholesterol, triglycerides, serum glutamic oxaloacetic transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT), alkaline phosphatase (ALP), total protein, serum urea, creatinine and hematological indices in normal, treated and untreated diabetic rats was investigated. Induction of diabetes in male Wistar rats (200-250 g) resulted in increased levels of serum glucose, total cholesterol, triglycerides, SGOT, SGPT, ALP, urea and creatinine and decreased bodyweight, serum insulin, total protein, RBC, WBC and hemoglobin levels. Administration of the extract at three test doses resulted in significant reduction in the levels of serum glucose, total cholesterol, triglycerides, SGOT, SGPT, ALP, urea and creatinine and significant increase in the body weight, insulin, total protein, RBC, WBC and haemoglobin levels which were observed in STZ-induced diabetic rats after 21 days of the treatment with the extract. At 200 mg/kg, LCME showed significant (p˂0.01) reduction in plasma glucose levels compared to glibenclamide (10 mg/kg). The results indicate that methanolic extract of whole plant of Lindernia ciliata possesses significant antidiabetic activity. INTRODUCTION: Diabetes is a metabolic disorder characterized by chronic hyperglycemia and alterations in carbohydrate, protein and lipid metabolism with absolute or relative deficiencies in insulin secretion and/or insulin production 1 .

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Antidiabetic activity of hydro-alcoholic stem bark extract of Callicarpa arborea Roxb. with antioxidant potential in diabetic rats

Cited by 41 publications

References 56 publications

Bioactive Flavonoids from the Stem Bark of <em>Cordia dichotoma</em> Forst.: Identification, Docking and ADMET Studies

Bioactive Flavonoids from the Stem Bark of <em>Cordia dichotoma</em> Forst.: Identification, Docking and ADMET Studies

Phytochemical Evaluation and Anti-Diabetic Effects of Ethanolic Leaf Extract of Petersianthus macrocarpus on Streptozotocin-Induced Diabetic Rats

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