Antidiabetic Activity of Extract and Fractions of Castanopsis costata Leaves on Alloxan-induced Diabetic Mice

Abstract

Indonesia has the world's country second largest forest biodiversity, with 28,000 plant species, of which 2,500 species, including Castanopsis costata, commonly known as "Cep-cepan", are used as medicine. 9-10 C. costata belongs to the Fagaceae family and has been known to exhibit therapeutic activities, for instance, anti-fever, digestive issue alleviation, and analgesic properties. 11 According to previous studies, C. costata exhibits numerous biological properties, including antioxidant activity, 12 antiinflammatory activity, 13 and antimalaria activity. 10 Furthermore, in North Sumatra, the plant is frequently used to treat diabetes, however, there are no studies confirming this practice. Therefore, this study aims to evaluate the anti-diabetic activity of the extract and fractions of C. costata leaves. MATERIALS AND METHODS Plant material collection and preparationAbout 10 kg of fresh C. costata leaves were collected in Pancur Batu Districts, North Sumatra, and transported to the Central Laboratory, Buana Perjuangan Karawang University for cleaning, air drying, milling, and extraction. The Herbarium Unit of the Department of Biology, Faculty of Mathematics and Science, Universitas Padjadjaran later recognized the plant as C. costata (Code: 219/HB/04/2017). Reagents and instrumentsThis study induced diabetes in Swiss albino mice using alloxan monohydrate (Sigma Chemical

Evaluation of antioxidant and antipyretic effects of ethanolic extract of Cep-cepan leaves ( Castanopsis costata (Blume) A.DC)

“…This might be due to the active compounds contained in the extract. A previous study showed that EECC contains several secondary metabolites, including polyphenols, flavonoids, alkaloids, tannins, saponins, and triterpenes [23,24]. Furthermore, several studies have reported flavonoid compounds' antipyretic and antioxidant activities [25][26][27].…”

“…Indonesia has the second-largest forest biodiversity in the world, with 28,000 plant species, of which 2,500 species, including Castanopsis costata, commonly known as "Cep-cepan," are used medicinally [8]. C. costata is known to have therapeutic activity as an antipyretic, relieve digestive problems, analgesic, and antidiabetic [9,10]. Traditionally, this plant is often used to treat fever, but no studies have confirmed this effect.…”

Sosialisasi Pemanfaatan Tumbuhan Cep-cepan (Castanopsis costata) sebagai Terapi Adjuvan Diabetes Mellitus di SMK Farmasi Purwakarta

“…Indonesia merupakan negara kedua dengan keanekaragaman hayati terbesar di dunia dengan hutan tempat ditemukannya 28.000 spesies tumbuhan. Diperkirakan sekitar 2.500 jenis tumbuhan berpotensi berkhasiat obat (Elfahmi et al, 2014;Alkandahri et al, 2019). Castanopsis costata, atau yang biasa disebut dengan "Cep-cepan", merupakan salah satu tanaman yang digunakan secara empiris sebagai obat antidiabetes oleh masyarakat Sumatera Utara, Indonesia.…”

“…Castanopsis costata, atau yang biasa disebut dengan "Cep-cepan", merupakan salah satu tanaman yang digunakan secara empiris sebagai obat antidiabetes oleh masyarakat Sumatera Utara, Indonesia. Hasil penelitian terbaru saat ini telah melaporkan bahwa ekstrak dan fraksi daun Cep-cepan mempunyai efek antidibetes yang di uji secara in vivo (Alkandahri et al, 2021). Aktivitas antidibetes dari tumbuhan Cep-cepan diduga karena senyawa flavonoid yang terkandung didalamnya (Alkandahri et al, 2016).…”

Ethnopharmacological Study of Medicinal Plants in the Rawamerta Region Karawang, West Java, Indonesia

“…In Indonesia, several ethnic groups have started using allopathic medicines together with ethnomedicine [5]. There is still a lack of documentation on herbal medicinal plants, making it necessary Since 2002 to search for information on their ethnomedicine knowledge for the treatment of diseases.…”