Antidiabetic Activity of Different Fractions of Ethanolic Extract of Dactyloctenium aegyptium in Streptozotocin Induced Diabetic Rats

Abstract

Aims:The present study was aimed to evaluate the antidiabetic effect of n-hexane, Chloroform, Ethyl acetate and Methanolic fractions from ethanolic extract of Dactyloctenium aegyptium in streptozotocin induced diabetic rats. Study Design: The animals were divided into seven groups and each group consisted of six rats. Place and Duration of Study: Laboratory of Raghavendra Institute of Pharmaceutical Education& Research (RIPER), Anantapuramu and 35 days. Methodology: The animals were divided into seven groups and each group consisted of six rats. Group I-Untreated normal rats, Group II-Untreated diabetic rats, Group III-Diabetic rats treated with Gliclazide 4.5 mg/kg, p.o. for 30 days, Group IV-Diabetic rats treated with n-hexane fraction of Original Research ArticleEDA (NHF) 50 mg/kg, p.o. for 30 days, Group V-Diabetic rats treated with Chloroform fraction of EDA (CF) 50 mg/kg, p.o. for 30 days, Group VI: Diabetic rats treated with Ethyl acetate fraction of EDA (EAF) 50 mg/kg, p.o. for 30 days, Group VII: Diabetic rats treated with Methanolic fraction of EDA (MF) 50 mg/kg, p.o. for 30 days and the serum was separated and used for the estimation of glucose levels. On 31 st day of experiment, best fraction was investigated further for its action on insulin, Hb, HbA1c, oxidative parameters, body weight and cell integrity of pancreas. Results: Results indicated that animals treated with MF shown significant decrease in blood glucose, HbA1c, malondialdehyde levels and significant increase in insulin, Hb, SOD, catalase, reduced glutathione and body weight. Conclusion: It could be concluded that methanolic fraction of ethanolic extract of Dactyloctenium aegyptium has favourable effect in bringing down the severity of diabetes however necessary studies are required on characterization of active principles.

Lipophilic Metabolites of Spartina maritima and Puccinellia maritima Involved in Their Tolerance to Salty Environments

“…This is the first study involving S. maritima and P. maritima hexane extraction, so previously reported extraction yields are not available in the literature. Nonetheless, the extraction yields of other halophytic species such as Desmotachya bipinnata (L.) Stapf, [23] Cynodon dactylon, [24] Cenchrus ciliaris [25] and Dactyloctenium aegyptium [26] are available in the literature, with values ranging from 1.4 % to 3.8 %. The extraction yields obtained in the present study are significantly higher than these reported ones, so the extraction employed was well suited to these plants.…”

Ameliorative Impacts of Dactyloctenium aegyptium and Parapholis incurva Ethanolic Extracts on Sodium Fluoride Induced Pathological Alterations in the Thyroid Gland in Male Rats

“…The results of previous study by Nagarjuna et al [32] showed that animals treated with D. aegyptium showed significant decreases in blood glucose, HbA1c, and MDA levels, as well as significant increases in insulin, Hb, SOD, catalase, GSH, and body weight. D. aegyptium could be used to treat diabetes, according to the findings.…”

“…Carbohydrates, proteins, amino acids, saponins, flavonoids, tannins, terpenoids, and alkaloids were found in the hydroalcoholic extract. Several solvents of D. aegyptium extracts revealed the significant difference of the output elution [32]. Quantitative investigation indicated that D. aegyptium leaf extract included alkaloids 0.540 ± 0.083, phenols 0.246 ± 0.041, Saponins 1.120 ± 0.047, and tannins 0.430 ± 0.032 mg/g dry weight.…”

Halophytic Grasses, a New Source of Nutraceuticals? A Review on Their Secondary Metabolites and Biological Activities

“…The methanol fraction of the ethanol extract from D. aegyptium (at a dose of 50 mg/kg) decreased both hyperglycemia and ameliorated oxidative stress, which contributed to its antidiabetic activities [ 198 ]. These could be related to insulinimimetic and antioxidant effects of the extract [ 198 ]. The mechanism of action, as well as the active principles, are still not known [ 198 ].…”

“…These could be related to insulinimimetic and antioxidant effects of the extract [ 198 ]. The mechanism of action, as well as the active principles, are still not known [ 198 ]. The methanol extract (70% methanol) of D. bipinnata was proven useful in the restoration of euglycemic levels (250 and 500 mg/kg) [ 157 ] while the hydroalcoholic extract of E. crus-galli ’s grains exhibited significant antidiabetic activity in diabetic rats (400mg/kg and 200mg/kg) as well as antioxidant activity and both protection and regeneration of pancreatic β-cells [ 195 ].…”