Antidiabetic Activity of Benzopyrone Analogues in Nicotinamide-Streptozotocin Induced Type 2 Diabetes in Rats

Abstract

Benzopyrones are proven antidiabetic drug candidate in diabetic drug discovery. In this view novel synthetic benzopyrone analogues were selected for testing in experimental diabetes. Type 2 diabetes (T2D) was induced in Wistar rats by streptozotocin (60 mg/kg, i.p.) followed by nicotinamide (120 mg/kg i.p.). Rats having fasting blood glucose (FBG) >200 mg/dL, 7 days after T2D-induction, are selected for the study. Test compounds and standard treatment were continued for 15 days. FBG, oral glucose tolerance test (OGTT), and insulin tolerance test (ITT) were determined on 21st day after induction of T2D. Plasma lipids and serum insulin were estimated. Homeostatic model assessment (HOMA-IR) was then calculated from serum insulin. Rats were sacrificed and pancreas was isolated for histopathological observations. Oxidative stress markers were estimated in liver homogenate. Quercetin, a natural product with benzopyrone ring, showed significant hypoglycemic activity comparable to glibenclamide. Treatment with test compounds lowered the FBG and insulin resistance was significant alleviated as determined by OGTT, HOMA-IR, and ITT. There was significant normalisation of liver antioxidant enzymes compared to diabetic rats indicating that all the synthesised benzopyrone analogues are beneficial in reducing oxidative stress and are on par with the standard quercetin and glibenclamide in experimental T2D.

Effect of Coenzyme Q 10 on Blood Glucose Level in Streptozotocin Induced Diabetic Rats

“…Just 15 minutes before the streptozotocin injection, nicotinamide was injected intraperitoneally in a dose of 120 mg/kg (Nayak, 2014). To administer streptozotocin and nicotinamide intraperitoneally, animal was held with its ventrum exposed and head pointed downwards.…”

Effect of co-administration of voglibose and vildagliptin on diabetic albino rats

“…According to weight of rats of this test group, drug was titrated and administered using gavage tube. 8 For stability of streptozotocin, the ideal pH range to be obtained was 4.0 -4.5. For this, 31.5 ml of citric acid was mixed with 18.5 ml of sodium citrate and this volume was adjusted to 100ml by adding deionized water.…”

“…It was administered in strength of dose: 60 mg/kg. 8 For this, 480 mg of STZ powder was dissolved in 40 ml of freshly prepared ice cold 0.1 M citrate buffer, so that 1 ml contained 12 mg of STZ required for 200 gram of rat. This solution of STZ was titrated according to the required total dose per animal and was administered intraperitoneally using 1 ml disposable syringe with 26 gauge needle.…”

“…8 2.4 gram of nicotinamide powder was dissolved in 100 ml of deionized water, so that 1ml contained 24 mg of nicotinamide, required for 200 gram weight of rat. This solution of nicotinamide was titrated according to the required total dose per animal and was administered intraperitoneally using 1 ml disposable syringe with 26 gauge needle.…”

A Review of Benzopyran Derivatives in Pharmacotherapy of Breast Cancer

“…Literature reveals that benzopyrans are important chemical synthon, associated with a broad range of biological effects including antioxidant [3,4], anti-HIV [5,6], neuroprotective [7,8], antiepileptic [9,10], antimicrobial [11,12], antidiabetic [13,14], antihypertensive [15,16], and anticancer agents [17,18]. Among the diverse biological activities of benzopyrans, breast cancer is one of the most intriguing since the discovery of ormeloxifene [19], KBU2046 (Phase II) [20], and B43-genistein [21].…”