Antidepressant exposure reduces body size, increases fecundity and alters social behavior in the short-lived killifish Nothobranchius furzeri

Thoré, Eli S.J.; Philippe, Charlotte; Brendonck, Luc; Pinceel, Tom

doi:10.1016/j.envpol.2020.115068

Cited by 34 publications

(28 citation statements)

References 69 publications

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“…First of all, we found that males were heavier than females of the same age. This result confirms earlier obtained data [15,18,20,31,32]. Body masses of males progressively increased with age and old males were significantly heavier than young ones.…”

Section: Discussionsupporting

confidence: 92%

“…Turquoise killifish is a promising model species for neuroscience, psychopharmacology and ecotoxicology [18,19,[31][32][33][34][35]. Behavioral variation in males and females of killifish was shown in the emergence, open field, habitat choice, life skill tests [33] and diurnal activity [34].…”

Section: Discussionmentioning

confidence: 99%

“…The sequence of coordinates and the density map were used to evaluate (1) the distance travelled (cm); (2) the immobility time (%); (3) the mean distance from the tank's bottom (cm); the time spent (%) in ( 4) the lower and ( 5) the upper thirds of the tank,; and(6) the explored part of the tank (%). The fourth and fifth parameters were calculated as the ratios of the amount of fish-associated pixels in the lower and upper thirds of the tank, respectively, to the sum of fish-associated pixels in all three thirds [18,26,27]. The last parameter was calculated as the ratio of the amount of pixels visited by the fish to the total amount of pixels in the tank's water area [28].…”

Section: Novel Tank Diving Testmentioning

confidence: 99%

“…The brain's 5-HT system regulates the physiological functions and behavior of killifish. Indeed, chronic treatment with small doses of the 5-HT transporter blocker, fluoxetine, led to decreased body size, as well as increased fecundity and sociability in N. furzeri [18,19]. However, until now there was only one publication revealing no effect of sex and rearing conditions on the level and turnover of 5-HT in brains of 108-day-old N. furzeri [20].…”

Section: Introductionmentioning

confidence: 99%

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Age-Related Alterations in the Behavior and Serotonin-Related Gene mRNA Levels in the Brain of Males and Females of Short-Lived Turquoise Killifish (Nothobranchius furzeri)

2021

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Short-lived turquoise killifish (Nothobranchius furzeri) have become a popular model organism for neuroscience. In the present paper we study for the first time their behavior in the novel tank diving test and the levels of mRNA of various 5-HT-related genes in brains of 2-, 4- and 6-month-old males and females of N. furzeri. The marked effect of age on body mass, locomotor activity and the mRNA level of Tph1b, Tph2, Slc6a4b, Mao, Htr1aa, Htr2a, Htr3a, Htr3b, Htr4, Htr6 genes in the brains of N. furzeri males was shown. Locomotor activity and expression of the Mao gene increased, while expression of Tph1b, Tph2, Slc6a4b, Htr1aa, Htr2a, Htr3a, Htr3b, Htr4, Htr6 genes decreased in 6-month-old killifish. Significant effects of sex on body mass as well as on mRNA level of Tph1a, Tph1b, Tph2, Slc6a4b, Htr1aa, 5-HT2a, Htr3a, Htr3b, Htr4, and Htr6 genes were revealed: in general both the body mass and the expression of these genes were higher in males. N. furzeri is a suitable model with which to study the fundamental problems of age-related alterations in various mRNA levels related with the brains 5-HT system.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Novel Tank Diving Testmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Age-Related Alterations in the Behavior and Serotonin-Related Gene mRNA Levels in the Brain of Males and Females of Short-Lived Turquoise Killifish (Nothobranchius furzeri)

2021

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“…Elaborate protocols for acute and chronic ecotoxicity testing with N. furzeri have been developed (Philippe et al, 2018b). Recently, traditional ecotoxicological testing has been expanded by also including sensitive behavioural responses to more accurately assess the environmental impact of pollution, especially with regard to pharmaceutical contaminants (Thoré et al, 2018a(Thoré et al, , 2019(Thoré et al, , 2020. To date, however, a lack of fundamental baseline data and the "behavioural norm" of fish models hampers rigorous ecological risk assessment of chemical compounds, and examining how environmental conditions affect baseline behavioural expression is imperative (Harris et al, 2014;Thoré et al, 2018a;Tanoue et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Conspecific density and environmental complexity impact behaviour of turquoise killifish (Nothobranchius furzeri)

Thoré

Brendonck

Pinceel

2020

Journal of Fish Biology

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Fish models are essential for research in many biological and medical disciplines. With a typical lifespan of only 6 months, the Turquoise killifish (Nothobranchius furzeri) was recently established as a time-and cost-efficient model to facilitate whole-life and multigenerational studies in several research fields, including behavioural ecotoxicology. Essential information on the behavioural norm and on how laboratory conditions affect behaviour, however, is deficient. In the current study, we examined the impact of the social and structural environment on a broad spectrum of behavioural endpoints in N. furzeri. While structural enrichment affected only fish boldness and exploratory behaviour, fish rearing density affected the total body length, locomotor activity, boldness, aggressiveness and feeding behaviour of N. furzeri individuals. Overall, these results contribute to compiling a behavioural baseline for N. furzeri that increases the applicability of this new model species. Furthermore, our findings will fuel the development of improved husbandry protocols to maximize the welfare of N. furzeri in a laboratory setting.

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Impact of Fluoxetine on Herbivorous Zooplankton and Planktivorous Fish

Grzesiuk

Gryglewicz

Bentkowski

et al. 2022

Enviro Toxic and Chemistry

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The contamination of freshwater environments by pharmaceuticals is a growing problem. Modern healthcare uses nearly 3000 substances, many of which are designed to work at low dosages and act on physiological systems that have been evolutionarily conserved across taxa. Because drugs affect the organisms from different trophic levels, pharmaceutical pollution is likely to disturb species interactions. However, such effects are still only poorly understood. We investigated the impacts of environmentally relevant concentrations of the common drug fluoxetine (Prozac), an increasingly common contaminant of European waters, on predation behavior of crucian carp (Carassius carassius), a common planktivorous European fish, and the somatic growth of its prey, the water flea (Daphnia magna), a widespread planktonic crustacean. We exposed these two organisms to environmentally relevant levels of fluoxetine (360 ng L−1): the fish for 4 weeks and the water fleas for two generations. We tested the growth of the daphnids and the hunting behavior (reaction distance at which fish attacked Daphnia and feeding rate) of the fish under drug contamination. We found that Daphnia exposed to fluoxetine grew larger than a nonexposed cohort. The hunting behavior of C. carassius was altered when they were exposed to the drug; the reaction distance was shorter, and the feeding rate was slower. These effects occurred regardless of Daphnia size and the treatment regime they were subjected to. Our results suggest that contamination of freshwater environments with fluoxetine can disrupt the top‐down ecological control of herbivores by reducing the hunting efficiency of fish and, as a consequence, may lead to increases in cladoceran population numbers. Environ Toxicol Chem 2023;42:385–392. © 2022 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.

show abstract

Antidepressant exposure reduces body size, increases fecundity and alters social behavior in the short-lived killifish Nothobranchius furzeri

Cited by 34 publications

References 69 publications

Age-Related Alterations in the Behavior and Serotonin-Related Gene mRNA Levels in the Brain of Males and Females of Short-Lived Turquoise Killifish (Nothobranchius furzeri)

Age-Related Alterations in the Behavior and Serotonin-Related Gene mRNA Levels in the Brain of Males and Females of Short-Lived Turquoise Killifish (Nothobranchius furzeri)

Conspecific density and environmental complexity impact behaviour of turquoise killifish (Nothobranchius furzeri)

Impact of Fluoxetine on Herbivorous Zooplankton and Planktivorous Fish

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