Anticonvulsant Effect of Diazoxide against Dichlorvos‐Induced Seizures in Mice

Abstract

Dichlorvos, a synthetic organophosphate toxin, is used as pesticides. These toxins can be used as pesticides in farming and medicine for the devastation and/or elimination of ectoparasites of animals. Reports have shown that Dichlorvos generate seizure effects in various animals. Potassium channel opener is extensively used for medication of cardiovascular and other diseases. Studies have shown that potassium channel opener has anticonvulsant effects in different animal models. The goal of this study was to evaluate the effect of dizoxide on Dichlorvos-induced seizures in mice. In this research, the animals received different doses of Diazoxide (1, 2.5, 5, 10, and 20 mg/kg b.wt.) intraperitoneally 30 min before intraperitoneal injection of Dichlorvos (50 mg/kg b.w.t). After Dichlorvos injection, latency of clones, severity of seizure, and finally death as the fate were investigated. Results showed that Diazoxide dose-dependently decreased the severity of Dichlorvos-induced seizures, so that Diazoxide at a dose of 5 mg (the lowest, P < 0.05) and 20 mg/kg b.wt. (the highest, P < 0.001) has anticonvulsant effects. Thus, our data suggest that diazoxide as ATP-sensitive potassium channels opener has anticonvulsant activity against dichlorvas-induced seizure.

Diazoxide Improves Mitochondrial Connexin 43 Expression in a Mouse Model of Doxorubicin-Induced Cardiotoxicity

“…C57BL/6j mice were randomly divided in three groups ( n = 12 for each experimental group). The doses used were 10 mg/kg [ 11 ] for DOXO and 20mg/kg for DZX [ 44 ].…”

RETRACTED ARTICLE: Cromakalim, a Potassium Channel Opener, Ameliorates Organophosphate- and Carbamate-Induced Seizures in Mice

“…Seizures were evaluated and scored based on the staging system by McLean et al (5,17): stage 0, no abnormal behavior; stage 1, some abnormal behaviors including salivation, chewing, and pawing at the mouth; stage 2, dazed appearance, intermittent motionlessness, tremor, and/or bobbing of the head; stage 3, random and/or generalized jerks, similar to stage 2; stage 4, intermittent rearing on hind legs with forepaws extended without falling; stage 5, falling to the side or rear, same as stage 4; and stage 6, status epilepticus.…”

“…The cellular mechanisms through which these agents induce seizure are not fully understood (5). Consequently, there is no efficient treatment to manage patients with OP or carbamate-induced seizures.…”

Investigation of Physiological Role of Mitochondrial Katp Channel’s on Penicillin G Induced Experimental Epilepsy Model in Rats

“…Similar to this mechanism, this study shows that bepridil delays the onset of a seizure, when administered before a seizure, possibly by extending the duration of action potential, through a mechanism similar to that of myocardial cells. While bepridil protects the brain against ischemic damage by blocking K + outflow by blocking the sarcoplasmic channels, it also shows a protective effect at the cellular level by simultaneously opening mito KATP channels [13]. In this study, the seizure stopped in a short time compared to the control groups, when bepridil administered both before and during a seizure.…”

“…Bepridil blocks the channels on the cell membrane called sarcolemmal KATP channels, while opening the mitochondrial KATP channels [13]. So far, there were no studies on the effect of bepridil on epilepsy.…”