Anticonvulsant Activity of B2, an Adenosine Analog, on Chemical Convulsant-Induced Seizures

Li, Min; Kang, Ruixia; Shi, Jian‐Gong; Liu, Geng-Tao; Zhang, Jianjun

doi:10.1371/journal.pone.0067060

Cited by 28 publications

(11 citation statements)

References 40 publications

(41 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…6 Studies have shown that the adenosine A 1 agonist promoted increased latency of seizure onset, decreased seizure occurrence, shorter seizure duration, and reduced mortality rate in chemical-induced seizure models. 28 In our study, the A 1 receptor agonist, CPA, increased the latency to reach the tonicclonic seizure stage, demonstrating anticonvulsant properties in zebrafish. On the other hand, the pretreatment with adenosine A 1 antagonist, DPCPX, induced a lower latency to reach the tonic-clonic seizure stage, suggesting proconvulsant effects in zebrafish.…”

Section: Discussionsupporting

confidence: 56%

Role of Adenosine Signaling on Pentylenetetrazole-Induced Seizures in Zebrafish

Siebel

Menezes²,

Capiotti³

et al. 2015

Zebrafish

View full text Add to dashboard Cite

Adenosine is a well-known endogenous modulator of neuronal excitability with anticonvulsant properties. Thus, the modulation exerted by adenosine might be an effective tool to control seizures. In this study, we investigated the effects of drugs that are able to modulate adenosinergic signaling on pentylenetetrazole (PTZ)-induced seizures in adult zebrafish. The adenosine A 1 receptor antagonist 8-cyclopentyl-1,3-dipropylxanthine (DPCPX) decreased the latency to the onset of the tonic-clonic seizure stage. The adenosine A 1 receptor agonist cyclopentyladenosine (CPA) increased the latency to reach the tonic-clonic seizure stage. Both the adenosine A 2A receptor agonist and antagonist, CGS 21680 and ZM 241385, respectively, did not promote changes in seizure parameters. Pretreatment with the ecto-5¢nucleotidase inhibitor adenosine 5¢-(a,b-methylene) diphosphate (AMPCP) decreased the latency to the onset of the tonic-clonic seizure stage. However, when pretreated with the adenosine deaminase (ADA) inhibitor, erythro-9-(2-hydroxy-3-nonyl)-adenine (EHNA), or with the nucleoside transporter (NT) inhibitors, dipyridamole and S-(4-Nitrobenzyl)-6-thioinosine (NBTI), animals showed longer latency to reach the tonic-clonic seizure status. Finally, our molecular analysis of the c-fos gene expression corroborates these behavioral results. Our findings indicate that the activation of adenosine A 1 receptors is an important mechanism to control the development of seizures in zebrafish. Furthermore, the actions of ecto-5¢-nucleotidase, ADA, and NTs are directly involved in the control of extracellular adenosine levels and have an important role in the development of seizure episodes in zebrafish.

show abstract

Section: Discussionsupporting

confidence: 56%

Role of Adenosine Signaling on Pentylenetetrazole-Induced Seizures in Zebrafish

Siebel

Menezes²,

Capiotti³

et al. 2015

Zebrafish

View full text Add to dashboard Cite

show abstract

“…The picrotoxin-induced test was conducted according to the method described previously (Li et al, 2013). Mice were divided into eight groups of six animals each.…”

Section: Picrotoxin-induced Seizuresmentioning

confidence: 99%

GABAA receptor modulation and neuropharmacological activities of viscosine isolated from Dodonaea viscosa (Linn)

Irshad

Khan

Mohammad

et al. 2015

Pharmacology Biochemistry and Behavior

View full text Add to dashboard Cite

“…On the postsynaptic terminal, A 1 Rs enhance the activity of G-protein-coupled Kir channels, which cause hyperpolarization and consequently reduced excitability (Boison, 2012;Aronica et al, 2013). Consistently, A 1 R agonists possess anticonvulsant and neuroprotective effects (Dunwiddie and Worth, 1982;Barraco et al, 1984;Gouder et al, 2003;Li et al, 2013), while antagonists promote seizures and aggravates neuronal damage (Ault et al, 1987;Dunwiddie, 1990;Avsar and Empson, 2004;Vianna et al, 2005). Consistently, A 1 R agonists possess anticonvulsant and neuroprotective effects (Dunwiddie and Worth, 1982;Barraco et al, 1984;Gouder et al, 2003;Li et al, 2013), while antagonists promote seizures and aggravates neuronal damage (Ault et al, 1987;Dunwiddie, 1990;Avsar and Empson, 2004;Vianna et al, 2005).…”

Section: Role Of Astrocyte-derived Adenosine In Epilepsymentioning

confidence: 97%

Crucial Role for Astrocytes in Epilepsy

Bedner¹,

Steinhäuser²

2015

Colloquium Series on Neuroglia in Biology and Medicine: From Physiology to Disease

View full text Add to dashboard Cite

ii Colloquium Digital Library of Life SciencesThe Colloquium Digital Library of Life Sciences is an innovative information resource for researchers, instructors, and students in the biomedical life sciences, including clinicians. Each PDF e-book available in the Colloquium Digital Library is an accessible overview of a fast-moving basic science research topic, authored by a prominent expert in the field. Each book is intended as a time-saving pedagogical resource for scientists exploring new areas outside of their specialty. They are also excellent tools for keeping current with advances in related fields, as well as refreshing one's understanding of core research topics in the biomedical sciences.This series of e-books is dedicated to physiology and pathophysiology of neuroglia. It will be valuable for the researchers and workers in the field of neurobiology and medicine in general. Illustrations provided will be suitable for professional presentations and instructional materials by researchers, physicians, teachers and members of the pharmaceutical industry. As the topic of neuroglia is generally overlooked in the majority of neuroscience curricula, this series could fill a need for materials to be used in courses and/or seminars aimed at exploring the role of glia in the brain in health and disease.For published titles please see the website, www.morganclaypool.com/toc/ngl/1/1. iv ABSTRACTThis book summarizes current evidence of astrocyte dysfunction in epilepsy and discusses presumed underlying mechanisms. Epilepsy is characterized by the periodic occurrence of seizures. Currently available anticonvulsant drugs and therapies are insufficient to controlling seizures in about one third of patients. Thus, there is an urgent need for new therapies that prevent generation of the disorder and improve seizure control in individuals already afflicted. The vast majority of epileptic cases are of idiopathic origin with their underlying mechanisms being unclear. Neurosurgical specimens from patients presenting with mesial temporal lobe epilepsy (MTLE) demonstrate marked reactive gliosis. Since recent studies have implicated astrocytes in important physiological roles in the CNS, such as synchronization of neuronal firing, it is plausible that they may also have a role in seizure generation and/or seizure spread. In support of this view, various membrane channels, receptors, and transporters in astrocytic membranes are altered in the epileptic brain. Excitingly, recent evidence suggests that in the course of the pathogenesis of MTLE, these glial changes alter homeostatic network functions and temporally precede the alterations in neurons. These findings might eventually classify MTLE as a glial rather than a neuronal disorder, and identify astrocytes as promising new targets for the development of more specific antiepileptogenic therapeutic strategies. Although research on astrocytes in epilepsy is still in its infancy, this book clearly demonstrates a critical role of astrocytes in the disturbance of K + and transmitter...

show abstract

Anticonvulsant Activity of B2, an Adenosine Analog, on Chemical Convulsant-Induced Seizures

Cited by 28 publications

References 40 publications

Role of Adenosine Signaling on Pentylenetetrazole-Induced Seizures in Zebrafish

Role of Adenosine Signaling on Pentylenetetrazole-Induced Seizures in Zebrafish

GABAA receptor modulation and neuropharmacological activities of viscosine isolated from Dodonaea viscosa (Linn)

Crucial Role for Astrocytes in Epilepsy

Contact Info

Product

Resources

About