Anticomplement therapy

Thompson

et al. 2021

Preprint

Cardiomyopathy syndrome (CMS), caused by piscine myocarditis virus (PMCV), is a serious challenge to Atlantic salmon (Salmo salar L.) aquaculture. Regrettably, husbandry techniques are the only tool to manage CMS outbreaks, and no prophylactic measures are available at present. Early diagnosis of CMS is therefore desirable, preferably with non-lethal diagnostic methods, such as serum biomarkers. To identify candidate biomarkers for CMS, the protein content of pools of sera (4 fish/pool) from salmon with CMS (4 pools) and from clinically healthy salmon (3 pools) were compared using liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS). Overall, seven proteins were uniquely identified in the sera of clinically healthy salmon, while twenty seven proteins were unique to the sera of CMS salmon. Of the latter, 24 have been associated with cardiac disease in humans. These were grouped as leakage enzymes (creatine kinase, lactate dehydrogenase, glycogen phosphorylase and carbonic anhydrase); host reaction proteins (acute phase response proteins - haptoglobin, fibrinogen, α2-macroglobulin, ceruloplasmin; and complement-related proteins); and regeneration/remodelling proteins (fibronectin, lumican and retinol). Clinical evaluation of the suitability of these proteins as biomarkers of CMS, either individually or as part of a panel, is a logical next step for the development of early diagnostic tools for CMS.

Section: Host Reaction Proteinsmentioning

confidence: 99%

Proteomic Characterisation of Serum Proteins From Atlantic Salmon (Salmo salar L.) With Cardiomyopathy Syndrome (CMS)

Thompson

et al. 2021

Preprint

“…Protein complement factor B, usually know as factor B, is a serine protease (Hourcade et al, 1998), part of the alternative complement system pathway (Kulkarni & Afshar-Kharghan, 2008;Ricklin et al, 2010). Its presence in CMS+ serum is not surprising, as a systemic inflammatory response is to be expected during PCMV infection, where the complement cascade is likely to promote inflammatory cell recruitment and activation, and destruction of infected cardiomyocytes.…”

Section: Host Reaction Proteinsmentioning

confidence: 99%

Proteomic characterization of serum proteins from Atlantic salmon (Salmo salar L.) from an outbreak with cardiomyopathy syndrome

Journal of Fish Diseases

McLean

et al. 2021

Cardiomyopathy syndrome (CMS), caused by piscine myocarditis virus (PMCV), is a serious challenge to Atlantic salmon (Salmo salar L.) aquaculture. Regrettably, husbandry techniques are the only tool to manage CMS outbreaks, and no prophylactic measures are available at present. Early diagnosis of CMS is therefore desirable, preferably with non-lethal diagnostic methods, such as serum biomarkers. To identify candidate biomarkers for CMS, the protein content of pools of sera (4 sh/pool) from salmon a CMS outbreak (3 pools) and from clinically healthy salmon (3 pools) were compared using liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS). Overall, seven proteins were uniquely identi ed in the sera of clinically healthy sh, while twenty seven proteins were unique to the sera of CMS sh. Of the latter, 24 have been associated with cardiac disease in humans. These were grouped as leakage enzymes (creatine kinase, lactate dehydrogenase, glycogen phosphorylase and carbonic anhydrase); host reaction proteins (acute phase response proteinshaptoglobin, brinogen, α2-macroglobulin, ceruloplasmin; and complement-related proteins); and regeneration/remodelling proteins ( bronectin, lumican and retinol). Clinical evaluation of the suitability of these proteins as biomarkers of CMS, either individually or as part of a panel, is a logical next step for the development of early diagnostic tools for CMS.

“…Protein complement factor B, usually know as factor B, is a serine protease (Hourcade et al, 1998), part of the alternative complement system pathway (Kulkarni et al, 2008, Ricklin et al, 2010. Its presence in CMS+ serum is not surprising, as a systemic in ammatory response is to be expected during PCMV infection, where the complement cascade is likely to promote in ammatory cell recruitment and activation, and destruction of infected cardiomyocytes.…”

Section: Host Reaction Proteinsmentioning

confidence: 99%

Proteomic characterisation of serum proteins from Atlantic salmon (Salmo salar L.) from an outbreak with Cardiomyopathy Syndrome

McLean

et al. 2021

Preprint

Cardiomyopathy syndrome (CMS), caused by piscine myocarditis virus (PMCV), is a serious challenge to Atlantic salmon (Salmo salar L.) aquaculture. Regrettably, husbandry techniques are the only tool to manage CMS outbreaks, and no prophylactic measures are available at present. Early diagnosis of CMS is therefore desirable, preferably with non-lethal diagnostic methods, such as serum biomarkers. To identify candidate biomarkers for CMS, the protein content of pools of sera (4 fish/pool) from salmon a CMS outbreak (3 pools) and from clinically healthy salmon (3 pools) were compared using liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS). Overall, seven proteins were uniquely identified in the sera of clinically healthy fish, while twenty seven proteins were unique to the sera of CMS fish. Of the latter, 24 have been associated with cardiac disease in humans. These were grouped as leakage enzymes (creatine kinase, lactate dehydrogenase, glycogen phosphorylase and carbonic anhydrase); host reaction proteins (acute phase response proteins - haptoglobin, fibrinogen, α2-macroglobulin, ceruloplasmin; and complement-related proteins); and regeneration/remodelling proteins (fibronectin, lumican and retinol). Clinical evaluation of the suitability of these proteins as biomarkers of CMS, either individually or as part of a panel, is a logical next step for the development of early diagnostic tools for CMS.