Anticoagulant Resistance in Synanthropic Rodents in the Stara Zagora Region, Bulgaria

Zhelev, G.; Koev, K.; Dimitrov, Vladimir; Petrov, Vladimir

doi:10.2478/macvetrev-2019-0010

Cited by 5 publications

(12 citation statements)

References 14 publications

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“…A white square indicates that M. m. domesticus has been tested for Vkorc1 mutations related to AR resistance and was found to be negative (this study). A red triangle indicates that M. m. domesticus has been tested for resistance through a lethal feeding period test and was found to be resistant to ARs [5,8,20,25,26]. A white triangle indicates that M. m. domesticus has displayed Vkorc1 mutations related to AR resistance [20].…”

Section: Mus Musculus D-loop Haplotypes In Western Australiamentioning

confidence: 99%

“…A white triangle indicates that M. m. domesticus has displayed Vkorc1 mutations related to AR resistance [20]. A black triangle indicates that a blood clotting response test has been performed and M. m. domesticus was found to be resistant to ARs [26]. A blue triangle indicates that an assay of the VKOR enzyme has been carried out and resistance to ARs was determined [5].…”

Section: Mus Musculus D-loop Haplotypes In Western Australiamentioning

confidence: 99%

“…The absence of resistance-related alleles in the VKORC1 gene does not rule out the presence of resistance in Western Australia as this gene is not the only known mechanism of resistance to anticoagulant rodenticides. Cytochrome P450 2C9 (CYP2C9) metabolises anticoagulant rodenticides, and genetic variants are associated with the need for higher dosages [26,38]. As Cytochrome P450 also detoxifies other pollutants, such as pesticides, the presence of environmental pollutants in general could lead to an increase of cytochrome P450 activity and render rodents pre-adapted to rodenticides [39].…”

Section: Plos Onementioning

confidence: 99%

“…There is scarce information regarding the presence and distribution of resistance in rodent populations of Australia, and a complete absence of information for M. musculus populations on the Australian mainland [25,26]. In Western Australia, neither rats nor mice have been tested to quantify the levels of resistance to ARs despite the pervasive use of poisons by government agencies and the public, whose use of both FGARs and SGARs is unmonitored and unregulated [9].…”

Section: Introductionmentioning

confidence: 99%

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Mus musculus populations in Western Australia lack VKORC1 mutations conferring resistance to first generation anticoagulant rodenticides: Implications for conservation and biosecurity

et al. 2020

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Background Humans routinely attempt to manage pest rodent populations with anticoagulant rodenticides (ARs). We require information on resistance to ARs within rodent populations to have effective eradication programs that minimise exposure in non-target species. Mutations to the VKORC1 gene have been shown to confer resistance in rodents with high proportions of resistance in mice found in all European populations tested. We screened mutations in Mus musculus within Western Australia, by sampling populations from the capital city (Perth) and a remote island (Browse Island). These are the first Australian mouse populations screened for resistance using this method. Additionally, the mitochondrial D-loop of house mice was sequenced to explore population genetic structure, identify the origin of Western Australian mice, and to elucidate whether resistance was linked to certain haplotypes. Results No resistance-related VKORC1 mutations were detected in either house mouse population. A genetic introgression in the intronic sequence of the VKORC1 gene of Browse Island house mouse was detected which is thought to have originated through hybridisation with the Algerian mouse (Mus spretus). Analysis of the mitochondrial D-loop reported two haplotypes in the house mouse population of Perth, and two haplotypes in the population of Browse Island. Conclusions Both house mouse populations exhibited no genetic resistance to ARs, in spite of free use of ARs in Western Australia. Therefore weaker anticoagulant rodenticides can be employed in pest control and eradication attempts, which will result in reduced negative impacts on nontarget species. Biosecurity measures must be in place to avoid introduction of resistant house mice, and new house mouse subspecies to Western Australia.

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Section: Mus Musculus D-loop Haplotypes In Western Australiamentioning

confidence: 99%

Section: Mus Musculus D-loop Haplotypes In Western Australiamentioning

confidence: 99%

Section: Plos Onementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Mus musculus populations in Western Australia lack VKORC1 mutations conferring resistance to first generation anticoagulant rodenticides: Implications for conservation and biosecurity

et al. 2020

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“…A red triangle indicates that M. m. domesticus has been tested for resistance through a lethal feeding period test and was found to be resistant to ARs. [5,8,20,25,26] A white triangle indicates that M. m. domesticus has displayed Vkorc1 mutations related to AR resistance. [20] A black triangle indicates that a blood clotting response test has been performed and M. m. domesticus was found to be resistant to ARs.…”

Section: Resultsmentioning

confidence: 99%

Mus musculus populations in Western Australia lack VKORC1 mutations conferring resistance to first generation anticoagulant rodenticides: Implications for conservation and biosecurity

Duncan

Koenders

Burnham

et al. 2020

Preprint

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AbstractBackgroundHumans routinely attempt to manage pest rodent populations with anticoagulant rodenticides (ARs). We require information on resistance to ARs within rodent populations to have effective eradication programs that minimise exposure in non-target species. Mutations to the VKORC1 gene have been shown to confer resistance in rodents with high proportions of resistance in mice found in all European populations tested. We screened mutations in Mus musculus within Western Australia, by sampling populations from the capital city (Perth) and a remote island (Browse Island). These are the first Australian mouse populations screened for resistance using this method. Additionally, the mitochondrial D-loop of house mice was sequenced to explore population genetic structure, identify the origin of Western Australian mice, and to elucidate whether resistance was linked to certain haplotypes.ResultsNo resistance-related VKORC1 mutations were detected in either house mouse population. A genetic introgression in the intronic sequence of the VKORC1 gene of Browse Island house mouse was detected which is thought to have originated through hybridisation with the Algerian mouse (Mus spretus). Analysis of the mitochondrial D-loop reported two haplotypes in the house mouse population of Perth, and two haplotypes in the population of Browse Island.ConclusionsBoth house mouse populations exhibited no genetic resistance to ARs, in spite of free use of ARs in Western Australia. Therefore weaker anticoagulant rodenticides can be employed in pest control and eradication attempts, which will result in reduced negative impacts on non-target species. Biosecurity measures must be in place to avoid introduction of resistant house mice, and new house mouse subspecies to Western Australia.

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Large‐scale identification of rodenticide resistance in Rattus norvegicus and Mus musculus in the Netherlands based on Vkorc1 codon 139 mutations

Krijger

Strating

Gent‐Pelzer³

et al. 2022

Pest Management Science

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BACKGROUND: Resistance to rodenticides has been reported globally and poses a considerable problem for efficacy in pest control. The most-documented resistance to rodenticides in commensal rodents is associated with mutations in the Vkorc1 gene, in particular in codon 139. Resistance to anticoagulant rodenticides has been reported in the Netherlands since 1989. A study from 2013 showed that 25% of 169 Norway rats (Rattus norvegicus) had a mutation at codon 139 of the Vkorc1 gene. To gain insight in the current status of rodenticide resistance amongst R. norvegicus and house mice Mus musculus in the Netherlands, we tested these rodents for mutations in codon 139 of the Vkorc1 gene. In addition, we collected data from pest controllers on their use of rodenticides and experience with rodenticide resistance. RESULTS: A total of 1801 rodent samples were collected throughout the country consisting of 1404 R. norvegicus and 397 M. musculus. In total, 15% of R. norvegicus [95% confidence interval (CI): 13-17%] and 38% of M. musculus (95% CI: 33-43%) carried a genetic mutation at codon 139 of the Vkorc1 gene.CONCLUSION: This study demonstrates genetic mutations at codon 139 of the Vkorc1 gene in M. musculus in the Netherlands. Resistance to anticoagulant rodenticides is present in R. norvegicus and M. musculus in multiple regions in the Netherlands. The results of this comprehensive study provide a baseline and facilitate trend analyses of Vkorc1 codon 139 mutations and evaluation of integrated pest management (IPM) strategies as these are enrolled in the Netherlands.

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Anticoagulant Resistance in Synanthropic Rodents in the Stara Zagora Region, Bulgaria

Cited by 5 publications

References 14 publications

Mus musculus populations in Western Australia lack VKORC1 mutations conferring resistance to first generation anticoagulant rodenticides: Implications for conservation and biosecurity

Mus musculus populations in Western Australia lack VKORC1 mutations conferring resistance to first generation anticoagulant rodenticides: Implications for conservation and biosecurity

Mus musculus populations in Western Australia lack VKORC1 mutations conferring resistance to first generation anticoagulant rodenticides: Implications for conservation and biosecurity

Large‐scale identification of rodenticide resistance in Rattus norvegicus and Mus musculus in the Netherlands based on Vkorc1 codon 139 mutations

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