Anticoagulant induced artefacts in peripheral blood smears

Narasimha, Aparna; Kumar, Harendra; Prasad, Csbr

doi:10.1007/s12288-008-0027-6

Cited by 9 publications

(8 citation statements)

References 4 publications

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“…These artefacts are likely to be caused by a lysolecithin formation or fall in Adenosine Triphosphate (ATP) as the blood is kept for a long time. 4 So the current study was undertaken to identify storage related morphological changes in blood cells so that these artefactual changes are not misinterpreted as pathologic findings.…”

Section: Introductionmentioning

confidence: 99%

Storage artefacts in peripheral blood smears

2020

JDPO

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Introduction: Microscopic evaluation of peripheral blood smear is a very important investigation. Blood for various laboratory analyses are commonly kept in ethylene diamine tetra acetic acid (EDTA). EDTA can however cause morphological and fragility changes in blood cells. However, morphological analyses can be greatly hampered due to occurrence of artefacts. Aims and Objectives: To identify the storage artefacts and thus avoid misinterpretation of peripheral blood smears. Materials and Methods: 50 blood samples were obtained using EDTA and 3.5% sodium citrate as anticoagulants. Smears were made immediately as well as after 2, 4 and 6hrs of storage at room temperature. Smears obtained from same patients by finger prick method served as controls. Conclusion: EDTA has been recommended as the anticoagulant of choice for peripheral smear but should be examined within 1hr of collection to avoid misinterpretation as pathological findings leading to wrong diagnosis.

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Section: Introductionmentioning

confidence: 99%

Storage artefacts in peripheral blood smears

2020

JDPO

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“…Hence, our observance is identical to study Brent L. wood et al 7 Causes for reduced WBC count can be cytoplasmic changes such as degranulation, vacuolization, degeneration, sometimes blebs formation & karyolitic changes & lobulations in their nucleus. 14 In relation with prior studies, it is stated that the statistically significant decrease in platelet count at all temperature begins after 6 hrs of sample collection. 2,15 While, in this present study, we have observed that platelet count also has reduced in stored blood samples at room temperature because of aggregation & swelling of platelets.…”

Section: Discussionmentioning

confidence: 75%

“…This observance is similar to other prior studies. 14 Hence, in order to get standard haematological results it is necessary to maintain blood samples at fixed 4 • C in conditions of extended period of evaluation, because previous studies proved that blood storage at 2 • C may lead to freezing injury to RBCs results in hemolysis. However, storage at > 6 • C lead to overgrowth of unspecified bacteria during sampling of blood.…”

Section: Discussionmentioning

confidence: 99%

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Study of refrigerated storage of blood at 4°C on automated hematological parameters & morphological changes in peripheral blood smear: A prospective study

Kadam

Patil²,

Mane³

2023

IJPO

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: Complete hemogram & peripheral blood smear are primary tests done in day to day practice for correct diagnosis & further treatment of patients. EDTA blood samples are used for this regular screening tests which shows less stability in case of delaying sampling. Hence, the final results of these tests can be affected by different storage conditions & duration. This present study provides data analyzed from EDTA blood samples stored upto 24 hour at both room temperature & 4°C refrigeration.This study includes total 150 blood samples from indoor & outdoor patient without any specific criteria which were collected randomly. These blood samples were analyzed using haematological analyzer for complete blood count & their peripheral smear after storage in both room temperature & 4°C refrigeration for 24 hrs. There was significant increase in MCV & decrease in MCHC, reduced WBC count & platelet count with storage at room temperature which was prohibited by refrigeration. However, both room temperature & refrigerated storage does not affect RBC count & hemoglobin.Blood samples stored at room temperature for 24 hrs results in changes in haematological parameters & morphology of cells. Hence, refrigerated storage at 4°C is recommended for accurate results in case evaluation of delayed samples.

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“…Previous studies on peripheral blood smear cited morphological changes began beyond 2hrs and are prominent after 6 hours. 9 We observed pronounced degenerative changes in smear of room temperature stored samples, which may be misinterpreted as pathological findings and thus leading to incorrect diagnosis. However by storing the blood samples at 4 0 C we could maintain the cell morphology.…”

Section: Discussionmentioning

confidence: 78%

Effect of room temperature and refrigerated storage on automated hematological parameters

D¹,

J²,

Eswari³

2020

IJPO

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For evaluating and monitoring of both inpatients and outpatients, complete blood count is one of the commonly performed investigations but at times it is challenging to evaluate all the blood samples on the collected day due to various factors like manpower shortages, weekends, and a single cell counter. Inspite of all these conditions we need to provide accurate report to patients. Hematology results are often influenced by the time between blood sampling and measurement as well as the storage conditions during sample delivery. This is because the hematological elements have limited stability in EDTA-anticoagulated blood. The stability of hematological parameters has improved after the anticoagulated blood is refrigerated.The aim is to investigate the effect of room temperature and refrigerated storage on complete blood counts and peripheral blood smear on automated hematological parameters. Blood samples were collected from 100 patients. Hematological parameters like Hemoglobin, PCV, MCV, MCH, MCHC, Platelet count, Red blood cell and White blood cell count were analyzed in room temperature storage and refrigerated storage at 40c for 24hrs using automated hematology analyzer. Peripheral blood smear from samples stored at room temperature and at 40 C in refrigerators for 24hrs were also examined.The study revealed RBC count and Hemoglobin were unaffected by storage at room temperature and refrigerated storage. Whereas MCV showed significant increase with storage at room temperature and WBC showed decrease in count with storage at room temperature that is preserved by refrigeration.

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Anticoagulant induced artefacts in peripheral blood smears

Cited by 9 publications

References 4 publications

Storage artefacts in peripheral blood smears

Storage artefacts in peripheral blood smears

Study of refrigerated storage of blood at 4°C on automated hematological parameters & morphological changes in peripheral blood smear: A prospective study

Effect of room temperature and refrigerated storage on automated hematological parameters

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