Anticlastogenic effect of caffeic acid phenethyl ester on cisplatin-induced chromosome aberrations in rat bone marrow cells

Yılmaz, H. Ramazan; Uz, Efkan; Altunbaşak, Ayşe; Sakallı, Esin; Özçelik, Nurten

doi:10.1177/0748233709355731

Cited by 18 publications

(4 citation statements)

References 29 publications

(40 reference statements)

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“…Previously, the synthesis technique of CAPE was reported by Yilmaz et al 15 Ready-made CAPE (Sigma-Aldrich, St Louis, MO, USA) was dissolved in absolute ethanol and further dilutions were made in saline. The systemic application dose of CAPE was chosen on the basis of the Grunberger et al 16 study.…”

Section: Methodsmentioning

confidence: 99%

Effect of caffeic acid phenethyl ester on bone formation in the expanded inter-premaxillary suture

Kazancioğlu¹,

Aksakalli²,

Ezirganlı³

et al. 2015

DDDT

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BackgroundNarrow maxilla is a common problem in orthodontics and dentofacial orthopedics. To solve this problem, a procedure called rapid maxillary expansion (RME) has been used. However, relapse tendency is a major problem of RME. Although relapse tendency is not clearly understood, various treatment procedures and new applications have been investigated. The present study aimed to investigate the possible effectiveness of caffeic acid phenethyl ester (CAPE) on new bone formation in rat midpalatal suture after RME.Materials and methodsTwenty male Sprague Dawley rats were used in this study. The animals were randomly divided into two groups as control and CAPE group. In the CAPE group, CAPE was administered systemically via intraperitoneal injection. RME procedure was performed on all animals. For this purpose, the springs were placed on the maxillary incisors of rats and activated for 5 days. After then, the springs were removed and replaced with short lengths of rectangular retaining wire for consolidation period of 15 days. At the end of the study, histomorphometric analysis was carried out to assess new bone formation.ResultsNew bone formation was significantly greater in the CAPE group than the control group (P<0.05). CAPE enhances new bone formation in midpalatal suture after RME.ConclusionThese results show that CAPE may decrease the time needed for retention.

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Section: Methodsmentioning

confidence: 99%

Effect of caffeic acid phenethyl ester on bone formation in the expanded inter-premaxillary suture

Kazancioğlu¹,

Aksakalli²,

Ezirganlı³

et al. 2015

DDDT

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“…Ayla et al showed that, in sperm samples incubated with CAPE, the DNA damage was decreased with low chromatin condensation when compared to samples not incubated with CAPE, and at the same time, incubation with CAPE reduced the MDA levels [21]. Yilmaz et al investigated the anticlastogenic effect of CAPE on CP-induced chromosomal defects in rat bone marrow cells [22]. First, CAPE was applied (10 µmol/kg), and then 24 h following that, CP (5 mg/kg) was applied.…”

Section: Discussionmentioning

confidence: 99%

Research on the protective effect of caffeic acid phenethyl ester on testicular damage caused by cisplatin

Ceylan¹,

Kaymak²,

Tan³

et al. 2020

Turk J Med Sci

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IntroductionChemotherapy drugs are used in the treatment of various types of cancers. Their toxicities and minor risks are often overlooked as a result of their potential usefulness in treatment. In particular, testicular cells have been selected as targets by chemotherapeutic agents, as they enter various mitotic, meiotic, and morphological processes and therefore they are easily damaged [1]. Cis-diamminedichloroplatinum (II) or cisplatin (CP), a highly effective antineoplastic DNA alkylating agent, has been used to treat a wide variety of solid tumors, such as those in the testicles, ovaries, breast, lungs, bladder, head, and neck [2]. However, the use of the drug is limited due to side effects, such as testicular toxicity, cachexia, and testicular damage [2,3]. When CP is administered at a high cumulative dose, it can result in permanent azoospermia and then infertility in patients [3]. CP interacts with DNA to create cross-link and intra-chain cross-links. Since the DNA modified by CP cannot be sufficiently renewed, the DNA damage initiates apoptosis [4].Caffeic acid phenethyl ester (CAPE) is one of the active ingredients of the sharp and fragrant propolis substance found in the extract collected by bees from plants. CAPE has been used as a folk remedy for many years [5]. Antimicrobial, antiinflammatory, immunomodulatory, antimutagenic, antioxidant, and anticarcinogenic effects of propolis have been demonstrated by various studies. CAPE, one of the active ingredients of propolis, and has an important place in these beneficial effects [6,7]. Although CAPE is a pharmacologically safe compound, it also reduces lipid peroxidation and stimulates antioxidant enzyme activity [8].Background/aim: Cisplatin (CP), a chemotherapeutic drug, causes damage to spermatogenic serial cells, Sertoli cells, and Leydig cells in rat testicles. It was aimed to investigate the protective effect of caffeic acid phenethyl ester (CAPE), one of the active ingredients of propolis, in eliminating CP-induced testicular damage.Materials and methods: Group 1 (control group) was given physiological saline solution intraperitoneally (IP) throughout the experiment. Group 2 (CP group) was given a single dose of CP (7 mg/kg) IP on the day 7. Group 3 (CP + CAPE group), was given CAPE (10 µmol/kg/day) IP for 12 days and a single dose of CP (7 mg/kg) IP on day 7. Group 4 (CAPE group) was given CAPE (10 µmol/kg/day) IP for 12 days. On day 14 of the experiment, the rats were decapitated under xylazine and ketamine anesthesia and their testicles were removed. The sections obtained from the testicles were stained with hematoxylin-eosin and histopathological damage was evaluated. Malondialdehyde (MDA) levels, and superoxide dismutase (SOD) and catalase (CAT) enzymatic activities were measured in the testicular tissue samples. Testosterone (TES) levels were measured in the blood serum. The Johnsen testicular biopsy score (JTBS) was used to evaluate testicular tubules. DNA damage was evaluated in sperm samples taken from the ductus epididymis using the come...

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“…The only exception was the HT29 cell line, where CAPE given along with CPT-11 increased genotoxicity compared to the series with CPT-11 alone, but the differences was not significant statistically. Yilmaz et al [50] analyzed rat bone marrow cells to see the impact of CAPE on cisplatin-induced chromosomal aberrations. CAPE induced a statistically significant decrease in the number of abnormal metaphases and chromosomal aberrations compared to the absence of CAPE.…”

Section: Discussionmentioning

confidence: 99%

“…CPT-11 was added to the medium at 5, 10, 15, 30, 60, and 100 µM, whereas for SN38 it was 0.25, 0.5, 1, 5, 10, and 15 µM. For each concentration of CPT-11 and SN38, CAPE was added at the concentration equal to the IC 50 determined for each of the tested cell lines. The results obtained for experimental series CAPE + CPT-11 and CAPE+SN38 are shown in Figures 3 and 4, respectively.…”

Section: Antagonistic Effect Of Cape On Cpt-11 and Sn38mentioning

confidence: 99%

Antagonistic Effects of CAPE (a Component of Propolis) on the Cytotoxicity and Genotoxicity of Irinotecan and SN38 in Human Gastrointestinal Cancer Cells In Vitro

et al. 2020

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The incidence of gastrointestinal cancers is increasing every year. Irinotecan (CPT-11), a drug used in the treatment of colorectal cancer and gastric cancer, is metabolized by carboxylesterases to an active metabolite, SN-38, which is more cytotoxic. CAPE (caffeic acid phenethyl ester) is an active component of propolis, which has a high antibacterial, antiviral, and antineoplastic potential. This study analyses the impact of CAPE on the cytotoxic (MTT assay), genotoxic (comet assay) and proapoptotic (caspase-3/7 activity) potential of irinotecan and its metabolite SN-38 in cultures of gastrointestinal neoplastic cells (HCT116, HT29, AGS). Cytotoxicity and genotoxicity activities of these compounds were carried out in comparison with human peripheral blood lymphocytes (PBLs) in vitro. The antioxidant potential of CAPE was investigated in relation H2O2-induced oxidative stress in the both neoplastic cells and PBLs. CAPE expressed cytotoxic, genotoxic, and pro-apoptotic activity against AGS, HCT116, and HT29 tumor cells. CAPE, in the presence of different concentrations of irinotecan or SN38, decreased the cytotoxicity, genotoxicity, and pro-apoptotic activity in these cell lines, but it has no such action on normal human peripheral blood lymphocytes.

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Anticlastogenic effect of caffeic acid phenethyl ester on cisplatin-induced chromosome aberrations in rat bone marrow cells

Cited by 18 publications

References 29 publications

Effect of caffeic acid phenethyl ester on bone formation in the expanded inter-premaxillary suture

Effect of caffeic acid phenethyl ester on bone formation in the expanded inter-premaxillary suture

Research on the protective effect of caffeic acid phenethyl ester on testicular damage caused by cisplatin

Antagonistic Effects of CAPE (a Component of Propolis) on the Cytotoxicity and Genotoxicity of Irinotecan and SN38 in Human Gastrointestinal Cancer Cells In Vitro

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