Anticholinergic, Antioxidant, and Antibacterial Properties of <i>Vitex Agnus‐Castus</i> L. Seed Extract: Assessment of Its Phenolic Content by LC/MS/MS

Kavaz, Arzu; Işık, Mesut; Dikici, Emrah; Yüksel, Mehmet

doi:10.1002/cbdv.202200143

Cited by 10 publications

(6 citation statements)

References 43 publications

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“…It was stated that samples with high total phenolic and flavonoid content showed high activity (Stavropoulou et al, 2021). There are numerous other studies in the literature that provide supportive evidence for this claim (Al-Khayri et al, 2022;Kavaz et al, 2022;Kavaz Yüksel et al, 2021;Tohma et al, 2015). According to the results obtained from our study, higher radical scavenging activity was found at the concentration of 30 μg mL −1 compared to other literature studies mentioned above.…”

Section: Devequisupporting

confidence: 82%

Antioxidant, antidiabetic effects and polyphenolic contents of propolis from Siirt, Turkey

Ören,

Tuncay,

Toprak

et al. 2024

Food Science & Nutrition

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Propolis, a natural product collected by honeybees from various plant sources, has gained significant attention due to its diverse bioactive compounds and potential therapeutic properties. To further explore its contents and biological activities, this study aimed to analyze the phenolic compounds in Siirt propolis extracts obtained using different solvents, namely ethanol, water, and ethanol‐water mixtures. The primary objective of this research was to investigate the phenolic profile, as well as the antidiabetic and antioxidant activities of the propolis extracts. Chemical profiling of extracts was performed using LC–MS/MS. The antioxidant potential of the propolis extracts was evaluated through free radical scavenging methods, including DPPH and ABTS assays. As a result of these analyses, propolis extracts showed moderate radical scavenging potential with 13.86%–35.72% for DPPH and 33.62%–62.50% for ABTS at a concentration of 30 μg mL−1, respectively. This radical scavenging potential of the extracts sheds light on its ability to combat oxidative stress, which is implicated in the development of diabetes, and its potential effects on cellular health. Additionally, the study assessed the antidiabetic properties of the propolis extracts by examining their inhibition effects on α‐amylase and α‐glycosidase enzymes. Extracts with high phenolic content showed a high inhibitory effect against α‐glucosidase with an IC50 of 5.72 ± 0.83 μg mL−1. This research provided significant findings regarding the potential use of propolis in the treatment of diabetes and related metabolic disorders.

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Section: Devequisupporting

confidence: 82%

Antioxidant, antidiabetic effects and polyphenolic contents of propolis from Siirt, Turkey

Ören,

Tuncay,

Toprak

et al. 2024

Food Science & Nutrition

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“…The absorbances of the mixtures were read at 700 nm to determine the iron-reducing capacity. Results were compared with standard reference compounds [ 63 , 64 ].…”

Section: Methodsmentioning

confidence: 99%

Phenolic Content Analysis of Two Species Belonging to the Lamiaceae Family: Antioxidant, Anticholinergic, and Antibacterial Activities

Ndhlala,

Işık,

Kavaz Yüksel

et al. 2024

Molecules

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The Lamiaceae family are utilized as ornamental, medicinal, and food supplements throughout the world. The current study focuses on a comparative analysis of the phenolic compositions and bioactivities (including antioxidant, anticholinergic, and antibacterial activities) of ethanolic extracts derived from the aerial parts of the two species (Lavandula stoechas L. and Thymus sipyleus Boiss). The presence of phenolic compounds and phytochemicals in the plant extracts was identified using the LC-MS/MS technique. The LC-MS/MS analysis revealed that vanillic acid (125,596.66 µg/L) was the most abundant phytochemical in L. stoechas. Kaempferol (8550.52 µg/L) was the most abundant substance in Thymus sipyleus. The assessment of the antioxidant efficacy of the species extracts was conducted using the DPPH (2.2-diphenyl-1-picryl-hydrazyl-hydrate), ABTS (2.2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)), Fe3+–Fe2+ reducing, and CUPRAC (Cu2+–Cu+ reducing) assays. The anticholinergic activity of the samples was determined using the acetylcholinesterase (AChE) inhibition assay. The results of antioxidant activity were higher in the T. sipyleus than in the L. stoechas ethanol extracts. The extracts of L. stoechas exhibited radical scavenging activity ranging from 15 to 18%, while T. sipyleus had activity effects ranging from 34% to 38%. The AChE inhibition potential for L. stoechas and T. sipyleus extracts as IC50 values were 0.221 ± 0.01 mg/mL and 0.067 ± 0.02 mg/mL, respectively. The antibacterial effects of the ethanolic extracts of these species against pathogenic bacteria isolates were determined using the MIC (minimal inhibitory concentration) method. These findings indicated that the extracts from L. stoechas and T. sipyleus possess the potential to be natural antioxidants in the realm of food preservation. Additionally, their antioxidant, anticholinergic, and antimicrobial properties suggest potential therapeutic utility in the management of certain diseases.

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“…Plants, which possess medicinal properties, can be employed as therapeutic or preventive solutions for various health issues (Kavaz et al, 2022). The lemon (Citrus × limon (L) Burm.…”

Section: )mentioning

confidence: 99%

Protective Effects of Lemon Leaf and Limonene against Indomethacin-Induced Gastric Ulcers in Rats: An In Vivo and In Silico Study

Aggul,

Duysak,

Gulaboglu

et al. 2024

Preprint

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This paper aims to assess the possible protective effects of the lemon leaves and its main component, limonene, which is known for its active principles and health benefits, against indomethacin-induced oxidative stress in the rats. In this study, the rats were given the ethanol extract from the lemon (Citrus L.) leaf and famotidine, a medication used for treating duodenal and gastric ulcers. The extracts (0.25, 0.5, and 1 g/kg) and/or famotidine (20 mg/kg) were administered to the treated rats via oral gavage at the doses determined for each group. Five minutes later, the rats were orally given a single dose of indomethacin (25 mg/kg). After six hours, the stomach tissues of the rats were collected for biochemical analysis. The treatment significantly improved gastric ulcer conditions by reducing oxidative stress, a key contributor to ulcer formation. Notably, the rats that received the highest dose of the extract exhibited significantly reduced MDA levels, an indicator of oxidative stress, in comparison to the control group. Furthermore, these rats showed a significant increase in SOD activities and GSH levels. The present study also focused on the mechanism underlying the gastroprotective action of limonene, a primary component of Citrus L., against PGE2, Na+/K+-ATPase, and H+/K+-ATPase under in silico conditions. The findings from this study might have significant implications for treating numerous global health disorders due to the antioxidant properties of the ethanol extract from the lemon leaf.

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Anticholinergic, Antioxidant, and Antibacterial Properties of Vitex Agnus‐Castus L. Seed Extract: Assessment of Its Phenolic Content by LC/MS/MS

Cited by 10 publications

References 43 publications

Antioxidant, antidiabetic effects and polyphenolic contents of propolis from Siirt, Turkey

Antioxidant, antidiabetic effects and polyphenolic contents of propolis from Siirt, Turkey

Phenolic Content Analysis of Two Species Belonging to the Lamiaceae Family: Antioxidant, Anticholinergic, and Antibacterial Activities

Protective Effects of Lemon Leaf and Limonene against Indomethacin-Induced Gastric Ulcers in Rats: An In Vivo and In Silico Study

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