Antichagasic effect of hemocyanin derived from antimicrobial peptides of penaeus monodon shrimp

Monteiro, Marília Lopes; Lima, Dânya Bandeira; Menezes, Ramon Róseo Paula Pessoa Bezerra de; Sampaio, Tiago Lima; Silva, Brenna Pinheiro; Nunes, João Victor Serra; Cavalcanti, Mariana Maciel; Morlighem, Jean-Étienne; Martins, Alice Maria Costa

doi:10.1016/j.exppara.2020.107930

Cited by 14 publications

(13 citation statements)

References 21 publications

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“…Interestingly, other spots ranging from 20 to 60 kDa were also identified as HMC, which should be HMC fragments or modification isoforms ( Figure 1 ). In combination with previous reports that HMC possessed polymorphisms including SNPs ( 30 , 31 ) and alternative splicing variant ( 32 ) and could generate various antimicrobial peptides ( 7 , 12 , 17 , 18 , 51 , 52 ) with resistance to pathogens infection, all these results indicated that HMC varied at nucleotide and protein levels.…”

Section: Discussionsupporting

confidence: 79%

“…Further research revealed that HMC may be a novel and important non-specific innate immune defense molecule (2,3). It has been reported that HMC could be functionally converted into phenoloxidase (4) antimicrobial peptides (5)(6)(7)(8), antiviral (9)(10)(11)(12), antitumor agent (13)(14)(15)(16)(17), antifungal (18), agglutinin (19,20), hemolysin (21), and regulatory proteins (22,23). Moreover, our previous findings indicated that HMC from shrimp Litopenaeus vannamei reacted with human Ig as an antigen (5,19,24) and acted as an immune-enhancing protein (25,26).…”

Section: Introductionmentioning

confidence: 99%

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Protein Diversity and Immune Specificity of Hemocyanin From Shrimp Litopenaeus vannamei

et al. 2021

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Hemocyanin is an important non-specific innate immune defense molecule with phenoloxidase, antiviral, antibacterial, hemolytic, and antitumor activities. To better understand the mechanism of functional diversity, proteomics approach was applied to characterize hemocyanin (HMC) expression profiles from Litopenaeus vannamei. At first, hemocyanin was purified by Sephadex G-100 and DEAE-cellulose (DE-52) columns from shrimp serum, and 34 protein spots were identified as HMC on the 2-DE gels. Furthermore, we found that 9 HMC spots about 75 or 77 kDa were regulated by Streptococcus agalactiae and Vibrio parahaemolyticus infection at 6, 12, and 24 h. In addition, 6 different pathogen-binding HMC fractions, viz., HMC-Mix, HMC-Vp, HMC-Va, HMC-Vf, HMC-Ec, and HMC-Sa, showed different agglutinative and antibacterial activities. Moreover, lectin-blotting analysis showed significant differences in glycosylation level among HMC isomers and bacteria-binding HMC fractions. Particularly, the agglutinative activities of the HMC fractions were almost completely abolished when HMC was deglycosylated by O-glycosidase, which suggest that O-linked sugar chains of HMC played important roles in the innate immune recognition. Our findings demonstrated for the first time that L. vannamei HMC had molecular diversity in protein level, which is closely associated with its ability to recognize diverse pathogens, whereas glycan modification probably contributed to HMC’s diversity and multiple immune activities.

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Section: Discussionsupporting

confidence: 79%

Section: Introductionmentioning

confidence: 99%

Protein Diversity and Immune Specificity of Hemocyanin From Shrimp Litopenaeus vannamei

et al. 2021

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“…Most of the known AMPs come from the processing of larger inactive proteins; however, some studies suggest that biologically active proteins, such as hemocyanin [ 18 ] and hemoglobin [ 33 ], can be sources of AMPs. Hemocyanin-derived peptides with antimicrobial properties have previously been reported in shrimp [ 34 , 35 , 36 ], crayfish [ 18 ] and spiders [ 16 ]. The bibliographic review indicates that until 2015, the first AMPs derived from hemocyanin from mollusks were registered [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

Antibacterial, Antibiofilm and Anti-Virulence Activity of Biactive Fractions from Mucus Secretion of Giant African Snail Achatina fulica against Staphylococcus aureus Strains

et al. 2021

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Staphylococcus aureus is an important etiological agent that causes skin infections, and has the propensity to form biofilms, leading to significant mortality and morbidity in patients with wounds. Mucus secretion from the Giant African snail Achatina fulica is a potential source of biologically active substances that might be an important source for new drugs to treat resistant and biofilm-forming bacteria such as S. aureus. This study evaluated the effect of semi-purified fractions from the mucus secretion of A. fulica on the growth, biofilm formation and virulence factors of S. aureus. Two fractions: FMA30 (Mw >30 kDa) and FME30 (Mw 30−10 kDa) exhibited antimicrobial activity against S. aureus with a MIC50 of 25 and 125 µg/mL, respectively. An inhibition of biofilm formation higher than 80% was observed at 9 µg/mL with FMA30 and 120 µg/mL with FME30. Furthermore, inhibition of hemolytic and protease activity was determined using a concentration of MIC20, and FME30 showed a strong inhibitory effect in the formation of clots. We report for the first time the effect of semi-purified fractions of mucus secretion of A. fulica on biofilm formation and activity of virulence factors such as α-hemolysin, coagulase and proteases produced by S. aureus strains.

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“…Previous studies have proved that the immune activity of marine invertebrates’ hemocyanin ground on a variety of mechanisms, including glycosylation modification [ 9 ], strong phagocytic capacity [ 10 ], and enzymatic induction, that cause the generation of reactive oxygen species [ 11 ]. Recently, AMPs have been found to be derived from hemocyanin, which are constitutively synthesized in hemocytes and stored in granules [ 12 ]. Hemocyanin-derived AMPs have been studied in several invertebrates, such as the haliotisin peptide from the hemocyanin of abalones, which harbors bactericidal potential [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

“…Hemocyanin-derived AMPs have been studied in several invertebrates, such as the haliotisin peptide from the hemocyanin of abalones, which harbors bactericidal potential [ 13 ]. The Hmc364-382 peptide, derived from the hemocyanin of the shrimp Penaeus monodon , also showed promising activity against Trypanosoma cruzi [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Functional Characterization, Antimicrobial Effects, and Potential Antibacterial Mechanisms of NpHM4, a Derived Peptide of Nautilus pompilius Hemocyanin

et al. 2022

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Hemocyanins present in the hemolymph of invertebrates are multifunctional proteins that are responsible for oxygen transport and play crucial roles in the immune system. They have also been identified as a source of antimicrobial peptides during infection in mollusks. Hemocyanin has also been identified in the cephalopod ancestor Nautilus, but antimicrobial peptides derived from the hemocyanin of Nautilus pompilius have not been reported. Here, the bactericidal activity of six predicted peptides from N. pompilius hemocyanin and seven mutant peptides was analyzed. Among those peptides, a mutant peptide with 15 amino acids (1RVFAGFLRHGIKRSR15), NpHM4, showed relatively high antibacterial activity. NpHM4 was determined to have typical antimicrobial peptide characteristics, including a positive charge (+ 5.25) and a high hydrophobic residue ratio (40%), and it was predicted to form an alpha-helical structure. In addition, NpHM4 exhibited significant antibacterial activity against Gram-negative bacteria (MBC = 30 μM for Vibrio alginolyticus), with no cytotoxicity to mammalian cells even at a high concentration of 180 µM. Upon contact with V. alginolyticus cells, we confirmed that the bactericidal activity of NpHM4 was coupled with membrane permeabilization, which was further confirmed via ultrastructural images using a scanning electron microscope. Therefore, our study provides a rationalization for the development and optimization of antimicrobial peptide from the cephalopod ancestor Nautilus, paving the way for future novel AMP development with broad applications.

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Antichagasic effect of hemocyanin derived from antimicrobial peptides of penaeus monodon shrimp

Cited by 14 publications

References 21 publications

Protein Diversity and Immune Specificity of Hemocyanin From Shrimp Litopenaeus vannamei

Protein Diversity and Immune Specificity of Hemocyanin From Shrimp Litopenaeus vannamei

Antibacterial, Antibiofilm and Anti-Virulence Activity of Biactive Fractions from Mucus Secretion of Giant African Snail Achatina fulica against Staphylococcus aureus Strains

Functional Characterization, Antimicrobial Effects, and Potential Antibacterial Mechanisms of NpHM4, a Derived Peptide of Nautilus pompilius Hemocyanin

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