Anticariogenic Activities of Derris reticulata Ethanolic Stem Extract Against Streptococcus mutans

Pulbutr, Pawitra; Rattanakiat, Sakulrat; Phetsaardeiam, Natnicha; Modtaku, Patcharapa; Denchai, Rattazart; Jaruchotikamol, Achida; Khunawattanakul, Wanwisa

doi:10.3923/pjbs.2018.300.306

Cited by 6 publications

(3 citation statements)

References 18 publications

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“…Isolation of lupinifolin from Derris reticulata stem Derris reticulata stems were purchased from the local herb store in Bangkok, Thailand. The sample was authenticated according to a method previously described (26). A voucher specimen was deposited at the Herbarium unit of Pharmaceutical Chemistry and Natural Product Research, Faculty of Pharmacy, Mahasarakham University (code: MSU.PH-LEG-DR-01).…”

Section: Methodsmentioning

confidence: 99%

Antibiofilm and antibacterial activities of lupinifolin in combination with protein synthesis inhibitors against methicillin-resistant Staphylococcus aureus

Kwaengmuang,

Chaiyawong,

Warong

et al. 2023

J Herbmed Pharmacol

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Introduction: Methicillin-resistant Staphylococcus aureus (MRSA)-derived biofilm formation is a crucial virulence factor, which essentially contributes to therapeutic challenges. This study aims to evaluate the antibiofilm and antibacterial formation activities of lupinifolin, a prenylated flavanone derived from Derris reticulata Craib. stem, in combination with protein synthesis inhibitors. Methods: The crystal violet biofilm formation assay was performed to determine the biofilm formation activity. The synergistic antibacterial activities were evaluated using the checkerboard and time-kill assays. Results: Lupinifolin and tetracycline significantly reduced MRSA biofilm formation with IC50 values of 15.32 ± 5.98 and 13.42 ± 5.90 µg/mL, respectively. On the contrary, the individual treatment of streptomycin and clindamycin tended to enhance biofilm formation. Lupinifolin at the sub-MIC of 8 µg/mL in combination with certain sub-MICs of tetracycline (8 and 16 µg/mL), streptomycin (16, 32, and 64 µg/mL), or clindamycin (4, 8, and 16 µg/mL) caused significant inhibitions against MRSA biofilm formation (P<0.05). The combination of lupinifolin and streptomycin exhibited a synergy (FIC index <0.625), confirmed in the time-kill assay. Conversely, the combination of lupinifolin and tetracycline or clindamycin resulted in no interaction (FIC indices of 1.0078 and <1.0156, respectively). Conclusion: The antibacterial synergy of lupinifolin and streptomycin possibly contributed to their antibiofilm-forming activity. However, the combinations of lupinifolin and tetracycline or clindamycin conceivably executed their antibiofilm activity directly against the MRSA biofilm formation process. These findings indicate a potential role for lupinifolin as an antibiofilm enhancer to diminish MRSA biofilm formation.

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Section: Methodsmentioning

confidence: 99%

Antibiofilm and antibacterial activities of lupinifolin in combination with protein synthesis inhibitors against methicillin-resistant Staphylococcus aureus

Kwaengmuang,

Chaiyawong,

Warong

et al. 2023

J Herbmed Pharmacol

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“…Derris reticulata Craib, a climbing medicinal plant used in folk medicine [154], has prenylated flavanones as its main constituents, whose presence has been linked to several pharmacological outcomes, particularly antibacterial activity [154,155]. According to Pulbutr et al [155], the ethanolic extract from stems of D. reticulata was able to inhibit S. mutans DMST 1877 biofilm formation by up to 99.9%, at the highest tested concentration, 750 µg•µL −1 . These results are in accordance with the capability of D. reticulata to inhibit both sucrose-dependent and independent S. mutans adherence in a concentration dependent manner, at sub-MIC concentrations (<625 µg•µL −1 ).…”

Section: The Most Promising Medicinal Plant Extracts In the Control Of Oral Biofilmsmentioning

confidence: 99%

Antimicrobials from Medicinal Plants: An Emergent Strategy to Control Oral Biofilms

et al. 2021

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Oral microbial biofilms, directly related to oral diseases, particularly caries and periodontitis, exhibit virulence factors that include acidification of the oral microenvironment and the formation of biofilm enriched with exopolysaccharides, characteristics and common mechanisms that, ultimately, justify the increase in antibiotics resistance. In this line, the search for natural products, mainly obtained through plants, and derived compounds with bioactive potential, endorse unique biological properties in the prevention of colonization, adhesion, and growth of oral bacteria. The present review aims to provide a critical and comprehensive view of the in vitro antibiofilm activity of various medicinal plants, revealing numerous species with antimicrobial properties, among which, twenty-four with biofilm inhibition/reduction percentages greater than 95%. In particular, the essential oils of Cymbopogon citratus (DC.) Stapf and Lippia alba (Mill.) seem to be the most promising in fighting microbial biofilm in Streptococcus mutans, given their high capacity to reduce biofilm at low concentrations.

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“…The wood of this plant has been used in Thai folk medicine for throat diseases and diabetes, and as a cough suppressant, expectorant, and tonic agent. [11][12] Previous studies have shown that this plant also exerts estrogenic, 13 anticariogenic, 14 antibacterial, antiviral, anti-cancer, -glucosidase inhibitory, antidiabetic, and antioxidant activities. 11,[15][16] Moreover, it has also been shown that ethanol extract from the stem of D. reticulata significantly reduces nitric oxide (NO) production and the mRNA expression of pro-inflammatory cytokines of macrophages, which reveals its anti-inflammatory effect.…”

Section: Introductionmentioning

confidence: 99%

In vivo Anti-Inflammatory Activity of Derris reticulata Ethanol Extract

2021

TJNPR

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Derris reticulata Craib. has been used in Thai traditional medicine to treat various inflammatory diseases. Recently, D. reticulata extract has been reported to exert anti-inflammatory activity by inhibiting nitric oxide (NO) secretion and the expression of various mRNAs in stimulated macrophage cells. However, studies of its anti-inflammatory effects in an animal model have not been performed. The study evaluated the in vivo anti-inflammatory activity of an ethanol extract of the wood of D. reticulata (DRE). The anti-inflammatory effect of DRE on ethyl phenylpropiolate (EPP)-induced ear edema and carrageenan-induced paw edema in rats was studied. Furthermore, the NO scavenging activity and phytochemical contents of the DRE were determined. The results showed that the most abundant phytochemical compound was lupinifolin, followed by total proanthocyanidin, total phenolic compounds, and total flavonoids. DRE also exhibited NO scavenging activity (15.8 -49.7%) while 1%, 5%, and 10% DRE promoted a significant reduction of inflammation in EPP-induced rat ear edema (35.0 -52.5%) and carrageenan-induced rat paw edema (28.1 -41.0%). The study revealed that DRE possesses potent anti-inflammatory properties in animal models. The results confirm the efficacy of the traditional use of D. reticulata in the treatment of inflammatory diseases.

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Anticariogenic Activities of Derris reticulata Ethanolic Stem Extract Against Streptococcus mutans

Cited by 6 publications

References 18 publications

Antibiofilm and antibacterial activities of lupinifolin in combination with protein synthesis inhibitors against methicillin-resistant Staphylococcus aureus

Antibiofilm and antibacterial activities of lupinifolin in combination with protein synthesis inhibitors against methicillin-resistant Staphylococcus aureus

Antimicrobials from Medicinal Plants: An Emergent Strategy to Control Oral Biofilms

In vivo Anti-Inflammatory Activity of Derris reticulata Ethanol Extract

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