Anticarcinogenic Effects of Hexaamminecobalt(III) Chloride in Mice Initiated with Diethylnitrosamine

Abstract

Hexaamminecobalt(III) chloride ([Co(NH3)6]Cl3) was investigated for its antineoplastic role in relation to tumor marker enzymes, drug metabolizing enzymes, oxidative stress-related parameters, and histopathological analysis of liver and lung tissues of mice. Initiation was performed using a single intraperitoneal injection of diethylnitrosamine (DENA) at a carcinogenic dose of 90 mg/kg body weight. The cobalt complex supplementation at a dose of 100 ppm in drinking water was given ad libitum throughout the experimental period of 14 weeks. In comparison to lung, the cobalt complex supplementation was found to reverse DENA-induced biochemical changes more effectively in liver. Histological examination of liver and lung from DENA-initiated and cobalt-complex-supplemented mice showed considerable protection in the case of liver compared to that of lung. The involvement of the [Co(NH3)6]Cl3 in modulating several factors associated with carcinogenesis induced by DENA thus showed its anticarcinogenic potential against chemically induced hepatocarcinogenesis.

Studies on mode of action of hexaammine co(III) chloride against diethylnitrosamine‐induced hepatocarcinogenesis in mice

“…The study thus provides additional evidence to the already reported potential of the compound as the antineoplastic agent against chemical carcinogenesis by virtue of its ability to protect against liver injury caused by DENA [8].…”

“…We have previously reported that low-dose longterm administration of hexaammine cobalt(III) chloride may be exerting its anticarcinogenic effect by potentiating the antioxidant status of the cell [8]. The results of our present study show that cobalt complex may hinder the process of carcinogenesis by downregulating the signal transduction events involving NOS activity and DAG.…”

“…Group 1 served as the control group. Groups 2 and 3 mice were given a single carcinogenic intraperitoneal injection of DENA (90 mg/kg of body weight) in 0.9% NaCl solution as reported previously [8]. Two groups (Groups 1 and 2) were maintained on normal drinking water; whereas the other two groups (Groups 3 and 4) were supplemented with 100 ppm of the cobalt complex in drinking water ad libitum for a period of 14 weeks.…”

“…These data suggest that further investigations with more cobalt complexes are warranted. We have recently shown the anticarcinogenic potential of [Co(NH 3 ) 6 ]Cl 3 against diethylnitrosamine (DENA)-induced carcinogenesis [8]. In the present study, we have explored the possible mode of action of [Co(NH 3 ) 6 ]Cl 3 against DENA-induced carcinogenesis by evaluating liver function tests and diacylgylcerol (DAG)-associated signal transduction, as well as assessment of cell viability by using flow cytometry.…”

PARP inhibitor, olaparib ameliorates acute lung and kidney injury upon intratracheal administration of LPS in mice

“…The lungs were subjected to bronchoalveolar lavage (BAL) and fixed on microscopic slides as described [43]. Some groups of mice were sacrificed for procuring whole lung and kidney tissues for preparation of tissue homogenate as explained earlier [44]. The lungs of the mice sacrificed 6 h after LPS treatment were processed for extraction of total RNA and reverse transcribed to cDNA.…”