Anticandidal and Antibiofilm Effect of Synbiotics including Probiotics and Inulin-Type Fructans

Abstract

Background: There is great interest in the search for new alternatives to antimicrobial drugs, and the use of synbiotics is a promising approach to this problem. This study evaluated the growth inhibition and antibiofilm activity of the short-chain fatty acids produced by Lacticaseibacillus rhamnosus and Pediococcus acidilactici in combination with inulin-type fructans against Candida albicans. Methods: The growth inhibition of Candida was evaluated using microdilution analysis in 96-well microtiter plates; different concentrations of cell-free supernatants of Lacticaseibacillus rhamnosus and Pediococcus acidilactici were exposed to Candida albicans. The antibiofilm assessment was carried out using the crystal violet staining assay. The short-chain fatty acids were analyzed by gas chromatography. Results: The clinically isolated Candida albicans interacted with supernatants from Lacticaseibacillus rhamnosus and Pediococcus acidilactici and showed significant growth inhibition and antibiofilm formation versus the controls. Lactate and acetic acid were elevated in the supernatants. The results suggest that the supernatants obtained from the synbiotic combinations of Lacticaseibacillus rhamnosus and Pediococcus acidilactici with inulin-type fructans can inhibit the growth and biofilm formation against a clinically isolated Candida albicans strain. Conclusions: These results suggest that synbiotic formulations could be a promising alternative to antifungal drugs in candidiasis therapy.

Novel Dietary Approach with Probiotics, Prebiotics, and Synbiotics to Mitigate Antimicrobial Resistance and Subsequent Out Marketplace of Antimicrobial Agents: A Review

“… The synbiotic combinations inhibit the growth and biofilm formation of Candida albicans and could be used as an alternative to antifungal drugs in candidiasis therapy. Microtiter plates [ 139 ] L. rhamnosus with inulin or FOS Vancomycin susceptible Enterococcus faecalis (VSEF) and clinical vancomycin-resistant Enterococcus faecium (VREF) Synbiotic showed an inhibition effect on VREF growth, Lactobacillus rhamnosus with inulin being the most effective Agar well diffusion method [ 140 ] P. pentosaceus 5–33:3, L. mesenteroides 32–77:1, L. paracasei ssp. paracasei 19; and L. plantarum 2362; and inulin, oat bran, pectin, and resistant starch Critically ill, mechanically ventilated, multiple trauma patients Symbiotic treatment in critically ill, mechanically ventilated, multiple trauma patients improve the patient’s response by reducing infection and sepsis rates and Randomized Controlled Trial [ 141 ] L. plantarum ATCC-202195 plus FOS Sepsis in infants The synbiotic combination reduced culture-positive and culture-negative sepsis and lower respiratory tract infections.…”