Anticancerous efficacy of ayurvedic milk extract of &lt;i&gt;Semecarpus anacardium&lt;/i&gt; nuts on hepatocellular carcinoma in wistar rats

Joseph, JP; Raval, S. K.; Sadariya, KA; Jhala, M; Kumar, P

doi:10.4314/ajtcam.v10i5.12

Cited by 12 publications

(12 citation statements)

References 15 publications

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“…However in extract treatment group decrease in LDH level is significant at p<0.05 when compared to positive control and this is due to controlled glycolysis and protection to membrane integrity offered by the extract. 29 Agreeing with these results, similar results were obtained by Premalatha B and team 30 in HCC induced rat treated with Siddha preparation of the nut milk extract. This indicates the usefulness of this plant for anticancer properties.…”

Section: Histopathological Examination Of Hepatic Tissuessupporting

confidence: 74%

Antineoplastic Approach of Semecarpus anacardium Leaves Against N-Nitroso Diethylamine Initiated Hepatocellular Carcinoma

Upreti¹,

Sv²,

Das³

et al. 2018

IJPER

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Objective: To evaluate the effect of MESA on NDEA induced hepatocellular carcinoma on Sprague Dawley rats. Methodology: Semecarpus anacardium commonly known as 'marking nut tree' is a rich antioxidant and in Ayurveda it has been used for treatment of various forms of cancer. Methanolic extract of leaves of Semecarpus anacardium (MESA) was studied for its potential antioxidant property both in vitro and in vivo. NDEA 200 mg/kg, single i.p was the hepatocarcinogen while 200 mg/kg and 400 mg/kg of MESA was administered daily orally for 12 weeks. Protective effect of MESA was evaluated by measuring biochemical parameters like SGOT, SGPT, ALP and LDH. Alpha fetoprotein test was performed and histopathology changes of livers were assessed. Results: Preliminary phytochemical study revealed the presence of flavonoids, tannins, steroids, alkaloids, glycosides and vitamin C. MESA restored the level of antioxidants near to normal and reduced the elevated serum level of SGOT, SGPT, AST, LDH and AFP. The histopathology changes i.e. necrosis, widened sinusoids, elevated inflammatory cell infiltrate were partly or fully prevented in animals treated with the extract. Conclusion:The result of the present study indicates MESA to be rich in antioxidants and its active constituents mediated the free radical scavenging activity. 400 mg/kg extract showed promising effect in management of primary liver cancer.

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Section: Histopathological Examination Of Hepatic Tissuessupporting

confidence: 74%

Antineoplastic Approach of Semecarpus anacardium Leaves Against N-Nitroso Diethylamine Initiated Hepatocellular Carcinoma

Upreti¹,

Sv²,

Das³

et al. 2018

IJPER

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“…It also maintains normal physiological level of cyto-protective Glutathione enzyme (Mathivadhani et al, 2006), modulates tumor hypoxia associated factors (Panneerselvam Mathivadhani et al, 2007a) and extracellular matrix associated enzymes (P Mathivadhani et al, 2007b), leading to inhibition of tumor progression and metastasis in DMBA induced tumor bearing rats. Although, the milk of nut has reported to have anticancer property in hepatocellular carcinoma in wistar rats (Joice P Joseph et al, 2013). The nut extract is cytotoxic and induced cell death through apoptosis in breast cancer (T47D) cells by arresting the treated cells in G2/M phase (Panneerselvam Mathivadhani et al, 2007b).…”

Section: Discussionmentioning

confidence: 99%

“…Plant fruit part has been used in numerous Ayurvedic formulations since a time ago (Semalty et al, 2010). Recent studies have explored its various properties such as antioxidant (Ramprasath et al, 2005), antimicrobial (Purushothaman et al, 2017), hypoglycemic (Arul et al, 2004), anti-inflammatory (Khan et al, 2013), anticancerous (Joice P. Joseph et al, 2013), antiatherogenic (Semalty et al, 2010), etc. This study also advocate to the different properties of its nuts like antioxidant and cytotoxicity in cancer cells of breast, liver, lung, cervical and blood (Selvam et al, 2004; Semalty et al, 2010; Sharma et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Semecarpus anacardiumLinn. leaf extract exhibits activities against breast cancer and prolongs the survival of tumor-bearing mice

Singh¹,

Ranjan²,

Tripathi³

et al. 2020

Preprint

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Background: Semecarpus anacardium Linn. is a commonly used Ayurvedic medicinal plant. Their different parts including nuts, have various medicinal properties to treat clinical ailments such as vitiligo, inflamation, microbial infection, geriatric problem, baldness and neuro-related problems.Aim of the study: The main objective of this study was an evaluation of the anticancer activity of the leaves of Semecarpus anacardium Linn and phytochemical screening. Materials and methods: The phytochemical screening was carried out through GC-MS analysis. Cytotoxicity was examined using MTT assay whereas mode of cell death evaluated by fluorescence microscopy. Finally, antitumor activity was determined in EAC cell-induced tumorbearing mice. Results: The ethyl acetate extract induced cytotoxicity in cancer cells in a dose dependent manner (IC 50 : 0.57 µg/ml in MCF-7 cells) in different cancer cell lines. However, normal cells were relatively insensitive to the extract. Fluorescence microscopy results, confirmed that the extract induced apoptosis in cancer cells effectively. The extract induce cell cycle arrest at the G1 phase and suppressed cancer cell migration. An oral administration of the extract suppressed the tumor growth in the mice model bearing Ehrlich ascites carcinoma cells. Whereas, the ethyl acetate extract was also found to prolong the survival of tumor-bearing mice. Conclusion: Overall, these observations suggest the anticancer activities of the ethyl acetate extract of S. anacardium leaves. The study opens a new window to examine the phytochemical constituents from the leaves as a source for the anticancer compounds.

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“…The findings of the present study agree with the results of Joseph et al . [20] who evaluated the anticancerous efficacy of Ayurvedic milk extract of Semecarpus anacardium nuts on HCC in Wistar rats. Ayurvedic milk extract when administered showed a positive correlation with the action of doxorubicin.…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical, histopathological study and chemoprotective effect of Solanum nigrum in N-nitrosodiethylamine-induced hepatocellular carcinoma in Wistar rats

et al. 2018

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Background and AimCancer is a devastating disease with a severe impact on the physical and psychological well-being of patients. Hepatocellular carcinoma (HCC) has been reported in various species of animals including dogs, cats, sheep, and pigs. The present study aimed to study the immunohistochemical and histopathological changes andchemoprotective effect of aqueous and alcoholic extracts of Solanum nigrum on N-nitrosodiethylamine (NDEA)-induced HCC rat model.Materials and MethodsEighty-two male Wistar rats of 15 weeks of age weighing 200-250 g were selected for the experiment. They were randomly divided into ten groups. Group I served as normal control consisted of healthy rats. HCC was induced in Group II, IV, V, VI, VII, and X rats using NDEA as inducing agent followed by phenobarbitone as a promoter for 16 weeks. Group II rats were kept untreated as HCC control. Group III rats were kept as vehicle control (0.05% Sodium bicarbonate). Group IV and V rats were treated with aqueous extract of S. nigrum at 200 mg/kg and 400 mg/kg, respectively, and Group VI and VII rats were treated with an alcoholic extract of S. nigrum at 200 mg/kg and 400 mg/kg, respectively, daily orally for 28 days. Group X rats were treated withsorafenib as reference drug at a dose of 11.4 mg/kg daily orally for 28 days. Group VIII and IX rats were kept as aqueous and alcoholic extract control for studying the effect of the same on normal rats. Liver samples were collected to study the gross and histopathological lesions and the activity of cleaved caspase-3 and chemopreventive effect of aqueous and alcoholic extracts of S. nigrum on HCC.ResultsThe liver sections of rats from HCC control (Group II) showed loss of lobular architecture, necrosis, fatty change, enlarged and darkened nuclei with variable size, dilatation of hepatic sinusoids with Kupffer cell hyperplasia, dilatation and proliferation of bile duct, and intranuclear vacuoles and also showed the presence of more than one nucleolus. Administration of alcoholic extract of S. nigrum and sorafenib to NDEA/phenobarbital-treated rats reduced the severity of lesions in the liver. Immunohistochemical analysis of liver sections for caspase-3-positive cells of hepatic cancer-induced group showed immunoreactivity to rarely few. The immunoreactivity of the hepatocytes treated with a higher dose of alcoholic extract of S. nigrum was limited and was comparable to a standard drug, sorafenib.ConclusionOral administration of aqueous and alcoholic extracts of S. nigrum for 28 days showed significant rejuvenation in the structure of the liver in the histopathological section in a dose-dependent manner in rats.

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Anticancerous efficacy of ayurvedic milk extract of <i>Semecarpus anacardium</i> nuts on hepatocellular carcinoma in wistar rats

Cited by 12 publications

References 15 publications

Antineoplastic Approach of Semecarpus anacardium Leaves Against N-Nitroso Diethylamine Initiated Hepatocellular Carcinoma

Antineoplastic Approach of Semecarpus anacardium Leaves Against N-Nitroso Diethylamine Initiated Hepatocellular Carcinoma

Semecarpus anacardiumLinn. leaf extract exhibits activities against breast cancer and prolongs the survival of tumor-bearing mice

Immunohistochemical, histopathological study and chemoprotective effect of Solanum nigrum in N-nitrosodiethylamine-induced hepatocellular carcinoma in Wistar rats

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