Anticancer studies of the synthesized gold nanoparticles against MCF 7 breast cancer cell lines

Priya, M. R. Kamala; Iyer, Priya

doi:10.1007/s13204-014-0336-z

Cited by 49 publications

(8 citation statements)

References 4 publications

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“…Previous studies have also reported the antiproliferative potential of AuNPs in colon, 22 breast, 33 cholangiocarcinoma, 34 cervical, and ovarian 35 cancer cell lines and our current results are consistent with the above studies. Further, green synthesis of AuNPs using plants proved to be efficient even at 2 μg/mL inhibits the proliferation of breast cancer cells 33 . These studies show that regardless of the mode of synthesis (chemical, phyto, and microbial), AuNPs have the potential to inhibit the proliferation of cancer cells.…”

Section: Discussionsupporting

confidence: 93%

“…AuNPs smaller than 10 nm have effectively entered and accumulated in tumors cells and thereby induced cell death 4,32 . Previous studies have also reported the antiproliferative potential of AuNPs in colon, 22 breast, 33 cholangiocarcinoma, 34 cervical, and ovarian 35 cancer cell lines and our current results are consistent with the above studies. Further, green synthesis of AuNPs using plants proved to be efficient even at 2 μg/mL inhibits the proliferation of breast cancer cells 33 .…”

Section: Discussionsupporting

confidence: 92%

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An ecofriendly synthesized gold nanoparticles induces cytotoxicity via apoptosis inHepG2cells

Nandhini

Ezhilarasan

Rajeshkumar

2020

Environmental Toxicology

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Microbes have long been used for the synthesis of a variety of nanoparticles. Hepatocellular carcinoma (HCC) is the primary liver cancer and it is the second leading cause of cancer-related mortality worldwide. In this study, we have synthesized Enterococcus mediated gold nanoparticles (AuNPs) and investigated their cytotoxic potential against human hepatocellular cancer cell line (HepG2). AuNPs were synthesized using Enterococcus sp. RMAA. HepG2 cells were treated with different concentrations of AuNPs for 24 hours and cytotoxicity was analyzed by MTT ((4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. AuNPs induced reactive oxygen species expression was analyzed by 2 0 ,7 0-dichlorodihydrofluorescein diacetate staining. Morphological changes related to apoptosis was analyzed by annexin V/propidium iodide staining. Protein expression of proliferating cell nuclear antigen (PCNA) was done by western blotting analysis. Bacterial-mediated AuNPs caused significant cytotoxicity in HepG2 cells. AuNPs treatment also caused the significant expression of ROS and morphological damage related to apoptosis. AuNPs treatments were responsible for the dislocation of cytochrome c from mitochondria to cytosol. The protein expression of PCNA was significantly decreased upon AuNPs treatment. These findings suggest that Enterococcus-mediated AuNPs can inhibit the proliferation of HepG2 cells via intracellular ROS mediated apoptosis, decreased PCNA expressions, and it may have the potential to treat HCC.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 92%

An ecofriendly synthesized gold nanoparticles induces cytotoxicity via apoptosis inHepG2cells

Nandhini

Ezhilarasan

Rajeshkumar

2020

Environmental Toxicology

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“…22 Plant based biogenic method is one of the best candidates for largescale production of nanoparticles with well-defined size and morphology. 24 A variety of biogenic AuNPs, with different therapeutic properties such as vermifugal, 25 antibacterial, 26 antifungal 27 and anticancer, 28,29 have been recently reported. 22 The combination of inherent physicochemical properties of nanoparticles with those of biomolecules could led to the development of functionalized nano-biological constructs with attracting characteristics.…”

Section: Introductionmentioning

confidence: 99%

Gold nanoparticles as potent anticancer agent: green synthesis, characterization, and in vitro study

et al. 2016

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Biogenic high quality colloidal gold nanoparticles (AuNPs), as one of the best anticancer theranostic nanostructures among those reported until date, were synthesized via a simple, efficient and green chemical method using aqueous and ethanolic Taxus baccata extracts and comprehensively characterized by UV-vis spectroscopy, TEM, SEM, AFM, DLS, Zetasizer, EDS, and FT-IR techniques. The AuNPs, encapsulated by the ethanolic compounds of T. baccata with different shapes and uniform size of less than 20 nm and polydispersity index of 0.276, were found completely stable for several months. In consistent with the results of microscopic observations of cell morphology, MTT assay revealed a potent, selective, dose-and time-dependent anticancer activity of AuNPs on the prevalent cancer cell lines including breast (MCF-7), cervical (HeLa) and ovarian (Caov-4), more effective than most of the other reported similar cases. The detailed in vitro investigation of cell exposure by AuNPs, using flow cytometry and real-time PCR, indicated caspase-independent deathprogram as most probable anticancer mechanism of AuNPs. The overall results firmly indicated that the organic compounds of T. baccata, as appropriate reducing and stabilizing agents, not only significantly affect the physicochemical properties of AuNPs but also led to the green synthesis of potent anticancer agent with high potential for cancer therapy.

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“…Many plants (Camellia sinensis, Coriandrum sativum, Mentha arvensis, Phyl-lanthus amarus, Artabotrys hexapetalus, Mimusops elengi, Syzygium aromaticum) were described by Priya and Iyer, which showed that the green synthesized AuNPs have anticancer activity against the human breast cancer cell line, i.e., MCF7 and found that AuNPs at a minimum concentration of 2 µg/mL for cancer therapy are as effective as standard drugs. Moreover, the increase in the nanoparticle concentration is directly proportional to the effectiveness against cancer [131]. The increasing demand for biosynthesized gold nanoparticles has been greatly facilitated in medical applications, particularly in targeted drug delivery, one of the most recent advances in nanotechnology.…”

Section: Plant-based Synthesized Gold Nanoparticles As Anticancer Agentsmentioning

confidence: 99%

Green Synthesis of Gold Nanoparticles Using Plant Extracts as Beneficial Prospect for Cancer Theranostics

Bharadwaj

Rabha

Pati³

et al. 2021

Molecules

105

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Gold nanoparticles (AuNPs) have been widely explored and are well-known for their medical applications. Chemical and physical synthesis methods are a way to make AuNPs. In any case, the hunt for other more ecologically friendly and cost-effective large-scale technologies, such as environmentally friendly biological processes known as green synthesis, has been gaining interest by worldwide researchers. The international focus on green nanotechnology research has resulted in various nanomaterials being used in environmentally and physiologically acceptable applications. Several advantages over conventional physical and chemical synthesis (simple, one-step approach to synthesize, cost-effectiveness, energy efficiency, and biocompatibility) have drawn scientists’ attention to exploring the green synthesis of AuNPs by exploiting plants’ secondary metabolites. Biogenic approaches, mainly the plant-based synthesis of metal nanoparticles, have been chosen as the ideal strategy due to their environmental and in vivo safety, as well as their ease of synthesis. In this review, we reviewed the use of green synthesized AuNPs in the treatment of cancer by utilizing phytochemicals found in plant extracts. This article reviews plant-based methods for producing AuNPs, characterization methods of synthesized AuNPs, and discusses their physiochemical properties. This study also discusses recent breakthroughs and achievements in using green synthesized AuNPs in cancer treatment and different mechanisms of action, such as reactive oxygen species (ROS), mediated mitochondrial dysfunction and caspase activation, leading to apoptosis, etc., for their anticancer and cytotoxic effects. Understanding the mechanisms underlying AuNPs therapeutic efficacy will aid in developing personalized medicines and treatments for cancer as a potential cancer therapeutic strategy.

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Anticancer studies of the synthesized gold nanoparticles against MCF 7 breast cancer cell lines

Cited by 49 publications

References 4 publications

An ecofriendly synthesized gold nanoparticles induces cytotoxicity via apoptosis inHepG2cells

An ecofriendly synthesized gold nanoparticles induces cytotoxicity via apoptosis inHepG2cells

Gold nanoparticles as potent anticancer agent: green synthesis, characterization, and in vitro study

Green Synthesis of Gold Nanoparticles Using Plant Extracts as Beneficial Prospect for Cancer Theranostics

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