Anticancer potentiality and mode of action of low-carbohydrate proteins and peptides from mushrooms

Rezvani, Vala; Pourianfar, Hamid R.; Mohammadnejad, Safoora; Ansari, Alireza Madjid; Farahmand, Leila

doi:10.1007/s00253-020-10707-8

Cited by 15 publications

(6 citation statements)

References 111 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Lectins include fungal immunomodulatory proteins, ubiquitin-like proteins, enzymes, and unclassified proteins that are carbohydrate-free or possess less than 1–2% carbohydrate. The low-carbohydrate proteins and peptides (LCP) exert anticancer activities via specific pathways including DNase activity, endoplasmic reticulum stress, PI3K/Akt/mTOR-signaling pathway, and ubiquitin-mediated pathway, which are different from the common extrinsic or intrinsic apoptosis pathways [ 106 ].…”

Section: Mushroom Proteins Are Important Pharmaceutical Agentsmentioning

confidence: 99%

Nutritional Quality and Biological Application of Mushroom Protein as a Novel Protein Alternative

Ayimbila

Keawsompong

2023

Curr Nutr Rep

View full text Add to dashboard Cite

Purpose of Review Global concerns about population growth, economic, and nutritional transitions and health have led to the search for a low-cost protein alternative to animal origins. This review provides an overview of the viability of exploring mushroom protein as a future protein alternative considering the nutritional value, quality, digestibility, and biological benefits. Recent Findings Plant proteins are commonly used as alternatives to animal proteins, but the majority of them are low in quality due to a lack of one or more essential amino acids. Edible mushroom proteins usually have a complete essential amino acid profile, meet dietary requirements, and provide economic advantages over animal and plant sources. Mushroom proteins may provide health advantages by eliciting antioxidant, antitumor, angiotensin-converting enzyme (ACE), inhibitory and antimicrobial properties over animal proteins. Protein concentrates, hydrolysates, and peptides from mushrooms are being used to improve human health. Also, edible mushrooms can be used to fortify traditional food to increase protein value and functional qualities. These characteristics highlight mushroom proteins as inexpensive, high-quality proteins that can be used as a meat alternative, as pharmaceuticals, and as treatments to alleviate malnutrition. Summary Edible mushroom proteins are high in quality, low in cost, widely available, and meet environmental and social requirements, making them suitable as sustainable alternative proteins.

show abstract

Section: Mushroom Proteins Are Important Pharmaceutical Agentsmentioning

confidence: 99%

Nutritional Quality and Biological Application of Mushroom Protein as a Novel Protein Alternative

Ayimbila

Keawsompong

2023

Curr Nutr Rep

View full text Add to dashboard Cite

show abstract

“…Ubiquitin from mushrooms, such as Calvatia caelata and Ramaria botrytis has been previously reported for cytotoxic effects against MDA-MB-231 [ 55 ] and lung carcinoma A549 cell lines, respectively [ 56 ]. Rezvani et al reported the DNase activity of ubiquitin as one of the putative pathways to induce apoptosis [ 57 ]. Extracellular ubiquitin has been previously shown to induce apoptosis and suppress the growth of hematopoietic cells by involving the proteasome degradation pathway [ 58 ].…”

Section: Discussionmentioning

confidence: 99%

Proteomic characterization and cytotoxic potential of proteins from Cuscuta (Cuscuta epithymum (L.) crude herbal product against MCF-7 human breast cancer cell line

Akhtar,

Khurshid,

El-Aarag

et al. 2024

BMC Complement Med Ther

View full text Add to dashboard Cite

Background The burden of breast cancer, the second leading cause of death worldwide, is increasing at an alarming rate. Cuscuta, used in traditional medicine for different ailments, including cancer, is known for containing phytochemicals that exhibit anticancer activity; however, the bioactivities of proteins from this plant remain unexplored. This study aimed to screen the cytotoxic potential of proteins from the crude herbal product of Cuscuta epithymum(L.) (CE) harvested from the host plants Alhagi maurorum and Medicago sativa. Methods The proteins from CE were extracted using a salting-out method, followed by fractionation with a gel filtration chromatography column. Gel-free shotgun proteomics was subsequently performed for protein characterization. The viability assay using MTT was applied to deduce the cytotoxic potential of proteins against MCF-7 breast cancer cells, with further exploration of the effect of treatment on the expression of the apoptotic mediator BCL2-associated X protein (BAX) and B-cell lymphoma protein 2 (BCL-2) proteins, using western blotting to strengthen the findings from the in vitro viability assay. Results The crude proteins (CP) of CE were separated into four protein peaks (P1, P2, P3, and P4) by gel filtration chromatography. The evaluation of potency showed a dose-dependent decline in the MCF-7 cell line after CP, P1, P2, and P3 treatment with the respective IC50 values of 33.8, 43.1, 34.5, and 28.6 µg/ml. The percent viability of the cells decreased significantly upon treatment with 50 µg/ml CP, P1, P2, and P3 (P < 0.001). Western-blot analysis revealed upregulation of proapoptotic protein BAX in the cells treated with CP, P3 (P < 0.01), and P2 (P < 0.05); however, the antiapoptotic protein, BCL-2 was downregulated in the cells treated with CP and P3 (P < 0.01), but no significant change was detected in P2 treated cells. The observed cytotoxic effects of proteins in the CP, P1, P2, and P3 from the in vitro viability assay and western blot depicted the bioactivity potential of CE proteins. The database search revealed the identities of functionally important proteins, including nonspecific lipid transfer protein, superoxide dismutase, carboxypeptidase, RNase H domain containing protein, and polyribonucleotide nucleotidyltransferase, which have been previously reported from other plants to exhibit anticancer activity. Conclusion This study indicated the cytotoxic activity of Cuscuta proteins against breast cancer MCF-7 cells and will be utilized for future investigations on the mechanistic effect of active proteins. The survey of CE proteins provided substantial data to encourage further exploration of biological activities exhibited by proteins in Cuscuta.

show abstract

“…Recently, Rezvani et al (2020) have shown that peptides from mushrooms were active against cancer cells, so beside the methanolic and aqueous extracts, which have shown good antiradical scavenging activity, the peptide fraction was tested as well. Methanolic and aqueous extracts did not show cytotoxicity against either cancer or healthy cells (IC 50 >200 mg mL À1 ).…”

Section: Cytotoxicitymentioning

confidence: 99%

St. George’s mushroom, Calocybe gambosa (Fr.) Donk: A promising source of nutrients and biologically active compounds

Radović

Leković

Damjanović

et al. 2022

AAlim

View full text Add to dashboard Cite

The composition of biologically active compounds of Calocybe gambosa (Lyophyllaceae) was analysed, and the antioxidant and cytotoxic activities were tested in vitro. C. gambosa was low in energy, fat, and carbohydrates, but rich in proteins and fibres. The total polyphenol content in the extracts was low (23.08 ± 0.67 in aqueous and 24.99 ± 4.25 μg GA mg−1 of extract in methanolic extract). The methanolic extract showed anti-DPPH radical activity with an IC50 of 626.10 ± 25.20 μg mL−1. The sample of C. gambosa is rich in nucleotides and amino acids responsible for its pleasant taste. The nucleoside and 5′-monophosphates contents were 0.97 mg g−1 and 2.32 mg g−1 of dry mushroom, respectively. The contents of essential and non-essential amino acids were 18.41 ± 0.06 and 41.75 ± 0.30 mg g−1 d.w., respectively. The percentages of saturated and unsaturated fatty acids were 42.6% and 57.4%, respectively. The most abundant water-soluble and fat-soluble vitamins were B1 and E, respectively. Cytotoxic effect of the extracts was examined against different cancer cell lines, and the best cytotoxicity was showed by the peptide extract against colon cancer cell line LS174.

show abstract

Anticancer potentiality and mode of action of low-carbohydrate proteins and peptides from mushrooms

Cited by 15 publications

References 111 publications

Nutritional Quality and Biological Application of Mushroom Protein as a Novel Protein Alternative

Nutritional Quality and Biological Application of Mushroom Protein as a Novel Protein Alternative

Proteomic characterization and cytotoxic potential of proteins from Cuscuta (Cuscuta epithymum (L.) crude herbal product against MCF-7 human breast cancer cell line

St. George’s mushroom, Calocybe gambosa (Fr.) Donk: A promising source of nutrients and biologically active compounds

Contact Info

Product

Resources

About