Anticancer Effects of Washed-Dehydrated Solar Salt Doenjang on Colon Cancer-Induced C57BL/6 Mice

Lee, So‐Young; Hong, Geun-Hye; Chung, Ji Hyung; Park, Kun-Young

doi:10.1089/jmf.2023.k.0066

Cited by 3 publications

(3 citation statements)

References 55 publications

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“…Additionally, in comparison to PSD, GSD showed a 1.3-fold higher expression, whereas it was 2.7-fold lower compared to WDSD, indicating functional differences based on the type of solar salt used. This is consistent with research results showing that ingestion of WDSD effectively suppressed colon cancer by regulating the protein expression of various factors related to cancer cell development and growth, such as apoptosis and cell cycle arrest [20]. Yu et al [37] and Park et al [38] reported that the anticancer and antiobesity functionality of kimchi manufactured with washed and dehydrated sea salt was improved, because bittern was removed through washing and dehydration during the manufacturing process of sea salt, and minerals such as Mg and S were removed.…”

Section: Effects Of Wdsd On Mrna Expression Of the Apoptosisrelated G...supporting

confidence: 93%

“…After a one-week acclimation period, mice were categorized into (1) normal (Nor), (2) control (Con), (3) PSD, (4) GSD, and (5) WDSD groups. Except for the Nor group, all other groups were administered azoxymethane (AOM) intraperitoneally, followed by one week of providing dextran sodium sulfate (DSS) for their drinking water at 2 and 5 weeks thereafter [20]. The mouse diet was fed AIN-93G, and each sample group was prepared by adding 10% of the weight of the feed and allowed to freely consume diet and water.…”

Section: In Vivo Anticancer Effects Of Doenjangmentioning

confidence: 99%

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Anticancer Effects of Washed-Dehydrated Solar Salt Doenjang and its Metabolites

Lee,

Hong,

Park

2023

Preprint

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In this study, the anticancer effect of doenjang according to the type of salt was investigated. Three samples were prepared: doenjang made with purified salt (PSD), doenjang made with generally manufactured solar salt (GSD), and doenjang made with washed and dehydrated solar salt (WDSD). In mice in which colon cancer was induced with azoxymethane (AOM)/dextran sodium sulfate (DSS), doenjang made with solar salt, especially doenjang made with washed and dehydrated solar salt, was found to have a much higher colon cancer inhibition effect. WDSD significantly promoted the mRNA expression of apoptosis-related factors such as Bcl-2–associated X protein (Bax) and caspase 9 and the cell cycle arrest-related factors p53 and p21, and conversely significantly reduced the mRNA expression of apoptosis inhibitors such as B-cell lymphoma-2 (Bcl-2) (p < 0.05). Additionally, metabolites were investigated to determine which substances in WDSD exhibit this anticancer effect. As a result, the contents of isoflavone and soyasaponin B in the form of aglycons such as genistein, daidzein, and glycitein, which are known to have anticancer and anti-inflammatory properties, were found to be significantly high. Therefore, the results confirmed that doenjang prepared with washed and dehydrated solar salt has superior anticancer potential against colon cancer, and that various active ingredients contribute to the improvement of this functionality.

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Section: Effects Of Wdsd On Mrna Expression Of the Apoptosisrelated G...supporting

confidence: 93%

Section: In Vivo Anticancer Effects Of Doenjangmentioning

confidence: 99%

Anticancer Effects of Washed-Dehydrated Solar Salt Doenjang and its Metabolites

Lee,

Hong,

Park

2023

Preprint

Self Cite

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“…Then, DSS was provided again for a whole week. After nine weeks of this experiment, the mice were fasted for 12 h and sacrificed (Figure 12) [88][89][90].…”

Section: Animal Experiments Designmentioning

confidence: 99%

Broccoli Cultivated with Deep Sea Water Mineral Fertilizer Enhances Anti-Cancer and Anti-Inflammatory Effects of AOM/DSS-Induced Colorectal Cancer in C57BL/6N Mice

Lee,

Pan,

Lim

et al. 2024

IJMS

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This study aimed to determine the alleviating effect of broccoli grown with deep sea water mineral (DSWM) fertilizer extracted from deep sea water on the development of colorectal cancer in C57BL/6N mice treated with AOM/DSS. Naturaldream Fertilizer Broccoli (NFB) cultured with deep sea water minerals (DSWM) showed a higher antioxidant effect and mineral content. In addition, orally administered NFB, showed a level of recovery in the colon and spleen tissues of mice compared with those in normal mice through hematoxylin and eosin (H&E) staining. Orally administered NFB showed the inhibition of the expression of inflammatory cytokine factors IL-1β, IL-6, TNF, IFN-γ, and IL-12 while increasing the expression of IL-10. Furthermore, the expression of inflammatory cytokines and NF-κB in the liver tissue was inhibited, and that of inflammatory enzymes, such as COX-2 and iNOS, was reduced. In the colon tissue, the expression of p53 and p21 associated with cell cycle arrest increased, and that of Bcl-2 associated with apoptosis decreased. Additionally, the expression of Bax, Bad, Bim, Bak, caspase 9, and caspase 3 increased, indicating enhanced activation of apoptosis-related factors. These results demonstrate that oral administration of broccoli cultivated using DSWM significantly restores spleen and colon tissues and simultaneously inhibits the NF-κB pathway while significantly decreasing cytokine expression. Moreover, by inducing cell cycle arrest and activating cell apoptosis, they also suggest alleviating AOM/DSS-induced colon cancer symptoms in C57BL/6N mice.

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Anticancer effects of washed-dehydrated solar salt doenjang and its metabolites

Lee,

Hong,

Park

2024

J. Ethn. Food

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In this study, the anticancer effect of doenjang according to the type of salt was investigated. Three samples were prepared: doenjang made with purified salt, doenjang made with generally manufactured solar salt, and doenjang made with washed and dehydrated solar salt (WDSD). In mice in which colon cancer was induced with azoxymethane/dextran sodium sulfate, doenjang made with solar salt, especially doenjang made with washed and dehydrated solar salt, was found to have a much higher colon cancer inhibition effect. WDSD significantly promoted the mRNA expression of apoptosis-related factors such as Bcl-2–associated X protein (Bax) and caspase 9 and the cell cycle arrest-related factors p53 and p21, and conversely significantly reduced the mRNA expression of apoptosis inhibitors such as B-cell lymphoma-2 (Bcl-2) (p < 0.05). Additionally, metabolites were investigated to determine which substances in WDSD exhibit this anticancer effect. As a result, the contents of isoflavone and soyasaponin B in the form of aglycons such as genistein, daidzein, and glycitein, which are known to have anticancer and anti-inflammatory properties, were found to be significantly high. Therefore, the results confirmed that doenjang prepared with washed and dehydrated solar salt has superior anticancer potential against colon cancer, and that various active ingredients contribute to the improvement of this functionality.

show abstract

Anticancer Effects of Washed-Dehydrated Solar Salt Doenjang on Colon Cancer-Induced C57BL/6 Mice

Cited by 3 publications

References 55 publications

Anticancer Effects of Washed-Dehydrated Solar Salt Doenjang and its Metabolites

Anticancer Effects of Washed-Dehydrated Solar Salt Doenjang and its Metabolites

Broccoli Cultivated with Deep Sea Water Mineral Fertilizer Enhances Anti-Cancer and Anti-Inflammatory Effects of AOM/DSS-Induced Colorectal Cancer in C57BL/6N Mice

Anticancer effects of washed-dehydrated solar salt doenjang and its metabolites

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