Anticancer effects of the combined Thai noni juice ethanolic extracts and 5-fluorouracil against cholangiocarcinoma cells in vitro and in vivo

Prompipak, Jeerati; Senawong, Thanaset; Sripa, Banchob; Ketterman, Albert J.; Utaiwat, Suppawit; Woranam, Khanutsanan; Jeeunngoi, Jarckrit; Senawong, Gulsiri

doi:10.1038/s41598-021-94049-z

Cited by 9 publications

(8 citation statements)

References 43 publications

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“…Marmesinpossess multiple pharmacological functions including anti-inflammatory, antihepatotoxic and antitumor activities (63,64). Fluorouracil has anti-cancer effects (65). Moreover, we observed increased levels of L-palmitoylcarnitine, quinestrol, 7ketocholesterol, and tetrabromodiphenyl ether during thiram exposure.…”

Section: Discussionmentioning

confidence: 69%

Effects of thiram exposure on liver metabolism of chickens

Wang

Wang²,

Shabbir³

et al. 2023

Front. Vet. Sci.

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Pesticides are widely used to control crop diseases, which have made an important contribution to the increase of global crop production. However, a considerable part of pesticides may remain in plants, posing a huge threat to animal safety. Thiram is a common pesticide and has been proven that its residues in the feed can affect the growth performance, bone formation, and intestinal health of chickens. However, there are few studies on the liver metabolism of chickens exposed to thiram. Here, the present study was conducted to investigate the effect of thiram exposure on liver metabolism of chickens. Metabolomics analysis shows that 62 metabolites were down-regulated (ginsenoside F5, arbekacin, coproporphyrinogen III, 3-keto Fusidic acid, marmesin, isofumonisin B1, 3-Hydroxyquinine, melleolide B, naphazoline, marmesin, dibenzyl ether, etc.) and 35 metabolites were up-regulated (tetrabromodiphenyl ethers, deoxycholic acid glycine conjugate, L-Palmitoylcarnitine, austalide K, hericene B, pentadecanoylcarnitine, glyceryl palmitostearate, quinestrol, 7-Ketocholesterol, tetrabromodiphenyl ethers, etc.) in thiram-induced chickens, mainly involved in the metabolic pathways including glycosylphosphatidylinositol (GPI)-anchor biosynthesis, porphyrin and chlorophyll metabolism, glycerophospholipid metabolism, primary bile acid biosynthesis and steroid hormone biosynthesis. Taken together, this research showed that thiram exposure significantly altered hepatic metabolism in chickens. Moreover, this study also provided a basis for regulating the use and disposal of thiram to ensure environmental quality and poultry health.

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Section: Discussionmentioning

confidence: 69%

Effects of thiram exposure on liver metabolism of chickens

Wang

Wang²,

Shabbir³

et al. 2023

Front. Vet. Sci.

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“…It inhibited AGS cell proliferation with IC 50 of 35.59 µg/ml, which was only half the concentration required compared to the ethyl acetate extract of Celastrus orbiculatus (IC 50 68.24 mg/l or equivalent to 68.24 µg/ml) [ 71 ]. Previous research reported that the ethanolic extract of Thai noni juice Chiangrai ( Morinda citrifolia ) reduced viability KKU-213B cells with IC 50 values of 2.14 ± 0.08 mg/ml for 48 h, which was higher than 20-fold compared with ethyl acetate extract of Shorea roxburghii (91.60 µg/ml or equivalent to 0.09 mg/ml) [ 72 ]. After 48 h of treatment, ethyl acetate extract inhibited cell viability of HepG2 cells with IC 50 of 39.38 µg/ml, whereas other medicinal plant Thymus daenensis and Stachys pilifera showed higher IC 50 values of 210.2 ± 12 µg/ml and 109.7 ± 5 µg/ml, respectively [ 73 ].…”

Section: Discussionmentioning

confidence: 99%

Anticancer properties and metabolomic profiling of Shorea roxburghii extracts toward gastrointestinal cancer cell lines

Janthamala,

Promraksa,

Thanee

et al. 2024

BMC Complement Med Ther

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Background Gastrointestinal cancer (GIC) ranks as the highest cause of cancer-related deaths globally. GIC patients are often diagnosed at advanced stages, limiting effective treatment options. Chemotherapy, the common GIC recommendation, has significant disadvantages such as toxicity and adverse effects. Natural products contain substances with diverse pharmacological characteristics that promise for use in cancer therapeutics. In this study, the flower of renowned Asian medicinal plant, Shorea roxburghii was collected and extracted to investigate its phytochemical contents, antioxidant, and anticancer properties on GIC cells. Methods The phytochemical contents of Shorea roxburghii extract were assessed using suitable methods. Phenolic content was determined through the Folin-Ciocalteu method, while flavonoids were quantified using the aluminum chloride (AlCl3) method. Antioxidant activity was evaluated using the FRAP and DPPH assays. Cytotoxicity was assessed in GIC cell lines via the MTT assay. Additionally, intracellular ROS levels and apoptosis were examined through flow cytometry techniques. The correlation between GIC cell viability and phytochemicals, 1H-NMR analysis was conducted. Results Among the four different solvent extracts, ethyl acetate extract had the highest phenolic and flavonoid contents. Water extract exhibited the strongest reducing power and DPPH scavenging activity following by ethyl acetate. Interestingly, ethyl acetate extract demonstrated the highest inhibitory activity against three GIC cell lines (KKU-213B, HepG2, AGS) with IC50 values of 91.60 µg/ml, 39.38 µg/ml, and 35.59 µg/ml, while showing less toxicity to normal fibroblast cells. Ethyl acetate extract induced reactive oxygen species and apoptosis in GIC cell lines by downregulating anti-apoptotic protein Bcl-2. Metabolic profiling-based screening revealed a positive association between reduced GIC cell viability and phytochemicals like cinnamic acid and its derivatives, ferulic acid and coumaric acid. Conclusions This study highlights the potential of natural compounds in Shorea roxburghii in the development of more effective and safer anticancer agents as options for GIC as well as shedding light on new avenues for cancer treatment.

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“…TNJ extracts reduced hepatotoxicity in vivo associated with FFU. The treatment in KKU 100 implanted nude mouse with the drug alone or in combination with a high dose of TNJ showed toxicity on mouse liver as compared to low doses of TNJ with the drug which showed no adverse effect on mouse spleen and kidney (Prompipak et al, 2021). The administration of TNJ, alone or combined with the anticancer drug doxorubicin, for 14 days to female Balb‐c mice inoculated with Ehrlich ascites tumor cells, was reported to reduce the size of tumors (about 40–50% smaller in the treated group compared to the control group).…”

Section: Synergistic Effects Of Bioactive Noni Compounds In Combinati...mentioning

confidence: 99%

Anticancer and chemopreventive potential of Morinda citrifolia L. bioactive compounds: A comprehensive update

Kitic,

Miladinovic,

Randjelovic

et al. 2024

Phytotherapy Research

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Morinda citrifolia L., commonly known as Noni, has a longstanding history in traditional medicine for treating various diseases. Recently, there has been an increased focus on exploring Noni extracts and phytoconstituents, particularly for their effectiveness against cancers such as lung, esophageal, liver, and breast cancer, and their potential in cancer chemoprevention. This study aims to provide a comprehensive review of in vitro and in vivo studies assessing Noni's impact on cancer, alongside an exploration of its bioactive compounds. A systematic review was conducted, encompassing a wide range of scientific databases to gather pertinent literature. This review focused on in vitro and in vivo studies, as well as clinical trials that explore the effects of Noni fruit and its phytoconstituents—including anthraquinones, flavonoids, sugar derivatives, and neolignans—on cancer. The search was meticulously structured around specific keywords and criteria to ensure a thorough analysis. The compiled studies highlight Noni's multifaceted role in cancer therapy, showcasing its various bioactive components and their modes of action. This includes mechanisms such as apoptosis induction, cell cycle arrest, antiangiogenesis, and immune system modulation, demonstrating significant anticancer and chemopreventive potential. The findings reinforce Noni's potential as a safe and effective option in cancer prevention and treatment. This review underscores the need for further research into Noni's anticancer properties, with the hope of stimulating additional studies and clinical trials to validate and expand upon these promising findings.

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Anticancer effects of the combined Thai noni juice ethanolic extracts and 5-fluorouracil against cholangiocarcinoma cells in vitro and in vivo

Cited by 9 publications

References 43 publications

Effects of thiram exposure on liver metabolism of chickens

Effects of thiram exposure on liver metabolism of chickens

Anticancer properties and metabolomic profiling of Shorea roxburghii extracts toward gastrointestinal cancer cell lines

Anticancer and chemopreventive potential of Morinda citrifolia L. bioactive compounds: A comprehensive update

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