Anticancer Effects of Propionic Acid Inducing Cell Death in Cervical Cancer Cells

Pham, Chau Ha; Lee, Jooeun; Yu, Jinha; Lee, Sung Hoon; Yu, Kyung–Rok; Hong, Jaewoo; Cho, Namki; Kim, Seil; Kang, Dukjin; Lee, Soojin; Yoo, Hee Min

doi:10.3390/molecules26164951

Cited by 24 publications

(13 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Anticancer effects of SCFAs, especially butyrate, is well documented; its proper concentrations reduce proliferation and induce programmed cell death in CRC and other cancer cells [ 54 ]. Propionic acid can induce ROS generation, MMP depletion, and cell death (autophagy) in HeLa cells [ 55 ].…”

Section: Resultsmentioning

confidence: 99%

Anticancer Potential of Post-Fermentation Media and Cell Extracts of Probiotic Strains: An In Vitro Study

Nowak

Zakłos‐Szyda

Rosicka‐Kaczmarek

et al. 2022

Cancers

View full text Add to dashboard Cite

Background: Lactic acid bacteria (LAB), many of which are probiotics, can produce health-promoting metabolites (postbiotics). Purpose: To assess the mechanism of antiproliferative action of postbiotics, post-fermentation media (PFM) and cell extracts (CEs) of several strains of LAB were studied against colon (Caco-2), and cervix (HeLa) cancer cell lines, as well as normal intestine (IEC-6) cells, were used as a comparison. Methods: Postbiotics of various LAB (n = 39) were screened for their antiproliferative activity. The effect of PFM and CEs on reactive oxygen species (ROS), mitochondrial membrane potential (MMP), ATP production, phosphatidylserine (PS) externalisation, and apoptosis-related caspases 3/7 and 9 activation was assayed. Results: PFM and CEs showed strong dose-dependent antiproliferative activity against Caco-2 cells, up to 77.8 ± 0.8% and 58.4 ± 1.6% for PFM and CEs, respectively. Stronger inhibitory activity against cancerous (Caco-2 and HeLa) cells than against normal (IEC-6) cells was observed. PFM were more inhibitory than CEs, and both generated oxidative stress in Caco-2 cells. PFM of L. plantarum 0991 and L. brevis 0983 induced apoptosis in Caco-2 cells by the mitochondrial signalling pathway. Conclusions: Anticancer activity of PFM and CEs of LAB, as well as the ability of apoptosis induction, is strain-specific.

show abstract

Section: Resultsmentioning

confidence: 99%

Anticancer Potential of Post-Fermentation Media and Cell Extracts of Probiotic Strains: An In Vitro Study

Nowak

Zakłos‐Szyda

Rosicka‐Kaczmarek

et al. 2022

Cancers

View full text Add to dashboard Cite

show abstract

“…41 Similarly, Pham et al reported similar results in a study that compared the viability of cervical cancer cell lines (CaSki, SiHa, and HeLa cells) with normal cells BEAS-2B after treatment with propionic acid at a differential concentration. 42 The volatilome of HNC in saliva samples showed lower levels of propionic acid in HNC patients compared with healthy controls. 39 In our study, we found that propionic acid levels were significantly lower in ST users compared to ST nonusers, which is in line with previous reports assuming that ST alters the gut microbiota and depletes the body of beneficial organic acids, which can lead to various disorders.…”

Section: Discussionmentioning

confidence: 95%

“…Propionic acid has been shown to reduce fatty acid accumulation in the liver and plasma, decrease food intake, have immunosuppressive effects, and increase tissue insulin sensitivity . Studies have revealed that propionic acid has anticancer properties, and its levels have decreased in various types of cancer, such as HNC and cervical cancer. , In their caecal contents, rats exposed to cigarette smoke showed a significant decrease in SCFA, i.e., propionic acid, butyric acid, and valeric acid . This result suggested that smoking cigarettes may alter the gut environment and affect the gut microflora in the host.…”

Section: Discussionmentioning

confidence: 99%

Urinary Metabolomics Identified Metabolic Perturbations Associated with Gutka, a Smokeless Form of Tobacco

Jorvekar

Jala

Rai

et al. 2023

Chem. Res. Toxicol.

View full text Add to dashboard Cite

Gutka, a form of smokeless tobacco, is widely used in the Indian subcontinent and in other regions of South Asia. Smokeless tobacco exposure is most likely to increase the incidence of oral cancer in the Indian population, and metabolic changes are a hallmark of cancer. The development of biomarkers for early detection and better prevention measures for smokeless tobacco users at risk of oral cancer can be aided by studying urinary metabolomics and offering insight into altered metabolic profiles. This study aimed to investigate urine metabolic alterations among smokeless tobacco users using targeted LC-ESI-MS/MS metabolomics approaches to better understand the effects of smokeless tobacco on human metabolism. Smokeless tobacco users’ specific urinary metabolomics signatures were extracted using univariate, multivariate analysis and machine learning methods. Statistical analysis identified 30 urine metabolites significantly associated with metabolomic alterations in humans who chew smokeless tobacco. Receiver operator characteristic (ROC) curve analysis evidenced the 5 most discriminatory metabolites from each approach that could differentiate between smokeless tobacco users and controls with higher sensitivity and specificity. An analysis of multiple-metabolite machine learning models and single-metabolite ROC curves revealed discriminatory metabolites capable of distinguishing smokeless tobacco users from nonusers more effectively with higher sensitivity and specificity. Furthermore, metabolic pathway analysis depicted several dysregulated pathways in smokeless tobacco users, including arginine biosynthesis, beta-alanine metabolism, TCA cycle, etc. This study devised a novel strategy to identify exposure biomarkers among smokeless tobacco users by combining metabolomics and machine learning algorithms.

show abstract

“…Once again, metabolomics has also played a key role in characterizing SCFAs and their antitumor effects, especially in colorectal cancer [203,204]. As shown through a number of studies, SCFAs, such as butyric acid, acetic acid, and propionic acid, can act as histone deacetylase inhibitors or as autophagy/apoptosis promoters [204,205]. The production of SCFAs is largely driven by gut microbiota which convert dietary fiber into these anti-oncogenic fatty acids.…”

Section: Connecting the Multiple Views Of Cancer Through Metabolomicsmentioning

confidence: 99%

Metabolomics and the Multi-Omics View of Cancer

Wishart

2022

Metabolites

View full text Add to dashboard Cite

Cancer is widely regarded to be a genetic disease. Indeed, over the past five decades, the genomic perspective on cancer has come to almost completely dominate the field. However, this genome-only view is incomplete and tends to portray cancer as a disease that is highly heritable, driven by hundreds of complex genetic interactions and, consequently, difficult to prevent or treat. New evidence suggests that cancer is not as heritable or purely genetic as once thought and that it really is a multi-omics disease. As highlighted in this review, the genome, the exposome, and the metabolome all play roles in cancer’s development and manifestation. The data presented here show that >90% of cancers are initiated by environmental exposures (the exposome) which lead to cancer-inducing genetic changes. The resulting genetic changes are, then, propagated through the altered DNA of the proliferating cancer cells (the genome). Finally, the dividing cancer cells are nourished and sustained by genetically reprogrammed, cancer-specific metabolism (the metabolome). As shown in this review, all three “omes” play roles in initiating cancer. Likewise, all three “omes” interact closely, often providing feedback to each other to sustain or enhance tumor development. Thanks to metabolomics, these multi-omics feedback loops are now much more evident and their roles in explaining the hallmarks of cancer are much better understood. Importantly, this more holistic, multi-omics view portrays cancer as a disease that is much more preventable, easier to understand, and potentially, far more treatable.

show abstract

Anticancer Effects of Propionic Acid Inducing Cell Death in Cervical Cancer Cells

Cited by 24 publications

References 62 publications

Anticancer Potential of Post-Fermentation Media and Cell Extracts of Probiotic Strains: An In Vitro Study

Anticancer Potential of Post-Fermentation Media and Cell Extracts of Probiotic Strains: An In Vitro Study

Urinary Metabolomics Identified Metabolic Perturbations Associated with Gutka, a Smokeless Form of Tobacco

Metabolomics and the Multi-Omics View of Cancer

Contact Info

Product

Resources

About