Anticancer effects and lysosomal acidification in A549 cells by astaxanthin from Haematococcus lacustris

Abstract

Astaxanthin (AXN) is known to have health benefits by epidemiological studies. Therefore, it is of interest to assess the effect of AXN (derived from indigenous unicellular green alga Haematococcus lacustris) to modulate cell cycle arrest, lysosomal acidification and eventually apoptosis using in vitro in A549 lung cancer cells. Natural extracts of astaxanthin were obtained by standardized methods as reported earlier and characterized by standard HPLC and MS. Treatment of A549 cells with AXN (purified fraction) showed significant reduction in cell viability (about 50%) as compared to crude extract at 50µM concentration. Thus, we show the anticancer effects and lysosomal acidification in A549 cells by Astaxanthin from Haematococcus lacustris for further consideration. Together, our results demonstrated the anticancer potential of AXN from Haematococcus lacustris, which is found to be mediated via its ability to induce cell cycle arrest, lysosomal acidification and apoptotic induction.

Anticancer Activity of Astaxanthin-Incorporated Chitosan Nanoparticles

“…Yin et al reported that astaxanthin reduces lipopolysaccharide (LPS)-induced inflammatory cytokines and then abrogates allogeneic T cell proliferation [13]. The anticancer efficacy of AST was also reported by several authors [14][15][16][17][18]. Natural AST showed inhibitory effects against the proliferation of renal clear cell carcinoma and placental growth factor expression [14].…”

“…Natural AST showed inhibitory effects against the proliferation of renal clear cell carcinoma and placental growth factor expression [ 14 ]. Ramamoorthy et al reported that AST induces cell cycle arrest, lysosomal acidification, and apoptosis of A549 cells [ 15 ]. Also, Tsuji et al reported that glioblastoma progression in a murine orthotopic model was suppressed by oral administration of AST [ 16 ].…”

Melanin pigments from sediment-associated Nocardiopsis sp. marine actinobacterium and antibacterial potential

“…[ 5 ] Our research team is involved in high-quality publications and has extensive knowledge to translate the experiments. [ 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 ] To date to the best of our knowledge, there are no proper reports available on marine actinobacteria Nocardiopsis sp. from which the melanin pigment is extracted.…”