Anticancer Effect of Enterocin A-Colicin E1 Fusion Peptide on the Gastric Cancer Cell

Fathizadeh, Hadis; Saffari, Mahmood; Esmaeili, Davoud; Moniri, Rezvan; Mahabadi, Javad Amini

doi:10.1007/s12602-021-09770-y

Cited by 19 publications

(15 citation statements)

References 42 publications

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“…Disorder in this pathway causes disease that can sometimes lead to the reproduction of abnormal cells. The apoptosis process activates caspases, especially caspase 3, in two pathways, internally and externally (6,7,(21)(22)(23). The results of flow cytometry also showed that cancer cells treated with modified carvacrol and anti-cancer peptide induced apoptosis significantly higher than untreated cells.…”

Section: Discussionmentioning

confidence: 97%

“…The results of flow cytometry also showed that cancer cells treated with modified carvacrol and anti-cancer peptide induced apoptosis significantly higher than untreated cells. Treatment of gastric cancer cells with the following combination at a concentration of 0.125 µg/mL induces apoptosis in this cell line (6,13,14,24).…”

Section: Discussionmentioning

confidence: 99%

“…Then 2 mL of culture medium was added. Falcon-containing cell suspension was sent to the tissue test laboratory in order to evaluate the extent of cell apoptosis by flow cytometry, and the results were analyzed (see the diagrams) (6). All tests were repeated twice.…”

Section: Evaluation Of Ags Cell Apoptosis With Modified Carvacrol By ...mentioning

confidence: 99%

“…Gastric cancer is a growth that is beyond the control of malignant cells in the gastric and is a type of cancer that grows slowly over the years; however, there are changes in the layers of the gastric prior to the development of the cancer (1). Gastric cancer is one of the most common cancers worldwide, especially in western countries, but its prevalence is increasing in some countries such as Iran, China, Ireland, and Chile (2)(3)(4)(5)(6)(7)(8).…”

Section: Introductionmentioning

confidence: 99%

“…These peptides are kind of novel bioactive peptides that could be used as anti-tumor drugs with several advantages, such as delivering the efficacy of a combined drug as well as having high specificity, high penetration capacity, and low toxicity for normal cells. The anti-cancer peptide sequence has been designed using AntiCP software, and carvacrol has been also synthesized chemically (6,18).…”

Section: Introductionmentioning

confidence: 99%

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Determination of the Antibacterial and Apoptotic Effects of Carvacrol and Anti-cancer Peptide

Elahi

Hekmati

Esmaeili

et al. 2022

Jentashapir J Cell Mol Biol

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Background: Treating gastric cancer and antibacterial remains a major challenge. There have been many reports about the positive effects of carvacrol and anti-cancer peptides contributing to cancer inhibition and antibacterial activities. Objectives: This study aimed to determine antibacterial and anti-cancer effects of modified carvacrol against AGS gastric cancer cell line by adopting the flow cytometry technique. Methods: The treatment of cells with modified carvacrol containing anti-cancer peptide was used to evaluate the apoptosis effect against the AGS cell line. The treatment of AGS cells was performed by adopting flow cytometry in order to evaluate the apoptosis. Disc diffusion and MIC methods were used to determine the antibacterial effects. Results: The results showed that cells treated with carvacrol and anti-cancer peptide at a concentration of 0.125 µg/mL induced a 31-fold apoptotic effect. Phosphate buffered saline was used as the control group for the treatment that induced a 63% apoptotic effect on the AGS cell line. The results of antibacterial activity suggested that the modified carvacrol had antibacterial properties against Staphylococcus aureus (MIC = 6 µg/mL, Growth inhibition zone with diameter of 20mm) and Escherichia coli (12 µg/mL, Growth inhibition zone with diameter 16). The results also revealed that the minimum inhibitory concentration of the compound was 6 µg/mL for Staphylococcus aureus, while this value was 12 µg/mL for Escherichia coli. Conclusions: Since the anti-cancer properties of modified carvacrol mixture with anti-cancer peptide induced 1.25 times more than phosphate-buffered saline, the above combination may have been used to treat and induce apoptotic activity against gastric cancer cell lines.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Evaluation Of Ags Cell Apoptosis With Modified Carvacrol By ...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Determination of the Antibacterial and Apoptotic Effects of Carvacrol and Anti-cancer Peptide

Elahi

Hekmati

Esmaeili

et al. 2022

Jentashapir J Cell Mol Biol

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Turbidimetric bioassays: A solution to antimicrobial activity detection in asymptomatic bacteriuria isolates against uropathogenic Escherichia coli

Kenneally,

Murphy,

Sleator

et al. 2024

MicrobiologyOpen

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Traditional bacteriocin screening methods often face limitations due to diffusion‐related challenges in agar matrices, which can prevent the peptides from reaching their target organism. Turbidimetric techniques offer a solution to these issues, eliminating diffusion‐related problems and providing an initial quantification of bacteriocin efficacy in producer organisms. This study involved screening the cell‐free supernatant (CFS) from eight uncharacterized asymptomatic bacteriuria (ABU) isolates and Escherichia coli 83972 for antimicrobial activity against clinical uropathogenic E. coli (UPEC) strains using turbidimetric growth methods. ABU isolates exhibiting activity against five or more UPEC strains were further characterized (PUTS 37, PUTS 58, PUTS 59, S‐07‐4, and SK‐106‐1). The inhibition of the CFS by proteinase K suggested that the antimicrobial activity was proteinaceous in nature, potentially bacteriocins. The activity of E. coli PUTS 58 and SK‐106‐1 was enhanced in an artificial urine medium, with both inhibiting all eight UPECs. A putative microcin H47 operon was identified in E. coli SK‐106‐1, along with a previously identified microcin V and colicin E7 in E. coli PUTS 37 and PUTS 58, respectively. These findings indicate that ABU bacteriocin‐producers could serve as viable prophylactics and therapeutics in the face of increasing antibiotic resistance among uropathogens.

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Recovery and Purification Techniques Used for AMPs Produced by Lactic Acid Bacteria

Chavarría-Hernández,

López-Cuellar,

Garrido-Escárcega

et al. 2024

Antimicrobial Peptides From Lactic Acid Bacteria

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Anticancer Effect of Enterocin A-Colicin E1 Fusion Peptide on the Gastric Cancer Cell

Cited by 19 publications

References 42 publications

Determination of the Antibacterial and Apoptotic Effects of Carvacrol and Anti-cancer Peptide

Determination of the Antibacterial and Apoptotic Effects of Carvacrol and Anti-cancer Peptide

Turbidimetric bioassays: A solution to antimicrobial activity detection in asymptomatic bacteriuria isolates against uropathogenic Escherichia coli

Recovery and Purification Techniques Used for AMPs Produced by Lactic Acid Bacteria

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