Anticancer Effect and Apoptosis Induction of Gambogic Acid in Human Leukemia Cell Line K562 In Vitro

Ouyang, Jian

doi:10.12659/msm.893004

Cited by 18 publications

(9 citation statements)

References 28 publications

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“…Normal control cells were peripheral blood mononuclear cells derived from healthy donors. These cell lines were maintained in RPMI-1640 culture medium (Hyclone, Logan, UT) containing 10% fetal bovine serum (FBS; Hyclone, Logan, UT) [15]. All cells were cultured in humidified atmosphere with 5% CO 2 at 37°C [9].…”

Section: Methodsmentioning

confidence: 99%

Inhibition of MicroRNA-149-5p Induces Apoptosis of Acute Myeloid Leukemia Cell Line THP-1 by Targeting Fas Ligand (FASLG)

Tian

Li²

2016

Med Sci Monit

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BackgroundThis study was aimed to reveal the role of miR-149-5p in acute myeloid leukemia (AML) cells apoptosis and the possible mechanism involved.Material/MethodsThe expression of miR-149-5p in leukemia cell lines, as well as the blood and bone marrow (BM) samples from leukemia patients, were monitored by reverse-transcription polymerase chain reaction (RT-PCR). AML cell line THP-1 was transfected with miR-149-5p mimic or inhibitor, and then cell apoptosis was determined using the APO Percentage assay kit. The target of miR-149-5p was predicted by using the microRNA.org database, and verified by RT-PCR, Western blot, and Dual-Luciferase reporter assays. Further, small interfering RNA (siRNA) against the target gene was co-transfected with miR-149-5p inhibitor, and then the cell apoptosis and the expression of apoptosis-related proteins were assessed.ResultsMiR-149-5p was significantly up-regulated in leukemia cell lines and samples from leukemia patients (P<0.01 or P<0.001), especially in THP-1 cells and samples from AML patients. Cell apoptosis was significantly decreased by miR-149-5p overexpression (P<0.01) and increased by miR-149-5p suppression (P<0.05). Fas Ligand (FASLG) was a direct target of miR-149-5p, and was negatively regulated by miR-149-5p. More importantly, the inductive effects of miR-149-5p suppression on cell apoptosis were abrogated by si-FASLG (P<0.01). Furthermore, the up-regulative effects of miR-149-5p suppression on the phosphorylated form of Fas-associated via death domain (p-FADD), caspase-8, caspase-2, caspase-3, and the cleaved forms of these caspases were abrogated by si-FASLG.ConclusionsInhibition of miR-149-5p can induce apoptosis in THP-1 cells. These inductive effects might be via targeting FASLG and activating FADD and caspases.

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Section: Methodsmentioning

confidence: 99%

Inhibition of MicroRNA-149-5p Induces Apoptosis of Acute Myeloid Leukemia Cell Line THP-1 by Targeting Fas Ligand (FASLG)

Tian

Li²

2016

Med Sci Monit

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“…Inhibited cell proliferation Down regulated the levels of nuclear factor-κB (NF-κB), BCL-2, phosphatidylinositol3-kinase (PI3K), c-myc, and phosphorylation of serine-threonine kinase (p-AKT) (Chen et al, 2015). ).…”

Section: Lung Cancermentioning

confidence: 99%

“…In acute myeloid leukemia cell lines of experimental subjects such as Jurket, HL-60 and MV4-11 cells, nano-emulsion encapsulated GA enhanced the efficacy rate in both in vivo and in vitro studies (Feng et al, 2018). Administration of gambogic acid more than 0.5 μM to human leukemia cell line K562 dose-dependently induced apoptosis, inhibited cell proliferation, and down regulated the levels of nuclear factor-κB (NF-κB), BCL-2, phosphatidylinositol3-kinase (PI3K), c-myc, and phosphorylation of serine-threonine kinase (p-AKT) (Chen et al, 2015).…”

Section: Blood Cancermentioning

confidence: 99%

Gambogic Acid as Anticancer Agent: A Review

Gilani¹,

Imran²,

Haq³

et al. 2020

Preprint

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Gambogic acid, a common traditional Chinese medicine and widely distributed throughout South China, Vietnam, Cambodia, and Thailand. It is prenylated xanthone which is the significant bioactive compound of gamboge. Gambogic acid is known as a strong apoptotic inducer in cancer cells. It has been found as strong anticancer agent against various types of cancer cells lines such as breast cancer, pancreatic, and cervical cancer. It induces apoptosis, down regulates the anti-apoptotic proteins (survivin and BCL2,) and down regulates the activities of P-glycoprotein in drug sensitive human breast MCF-7 and drug-resistant MCF-7/ADR cells. Similarly, it also exerts alteration in P13K, AKT, p21, MMP-2 &-9, and phosphorylated-AKT expressions. The current review highlights the anticancer and chemo-preventive perspectives of gambogic acid and its mechanistic role against human and animal cancers.

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“…The morphological study of apoptotic cells was performed according to the method of Chen et al [25] with a few modifications. In brief, cells (5 × 10 4 cells/well) were incubated for 48 h in the absence or presence of 25, 50, 100, and 200 μg/ml cWBC in a 24-well plate.…”

Section: Morphological Observation Of Apoptotic Cells By Phase-contra...mentioning

confidence: 99%

Siamese Crocodile White Blood Cell Extract Inhibits Cell Proliferation and Promotes Autophagy in Multiple Cancer Cell Lines

Phosri¹,

Jangpromma²,

Chang³

et al. 2018

Journal of Microbiology and Biotechnology

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Cancer represents one of the most significant threats to human health on a global scale. Hence, the development of effective cancer prevention strategies, as well as the discovery of novel therapeutic agents against cancer, is urgently required. In light of this challenge, this research aimed to evaluate the effects of several potent bioactive peptides and proteins contained in crocodile white blood cell extract (cWBC) against LU-1, LNCaP, PC-3, MCF-7, and CaCo-2 cancer cell lines. The results demonstrate that 25, 50, 100, and 200 µg/ml cWBC exhibits a strong cytotoxic effect against all investigated cell lines (IC 50 70.34−101.0 µg/ml), while showing no signs of cytotoxicity towards noncancerous Vero and HaCaT cells. Specifically, cWBC treatment caused a significant reduction in the cancerous cells' colony forming ability. A remarkable suppression of cancerous cell migration was observed after treatment with cWBC, indicating potent antimetastatic properties. The mechanism involved in the cancer cell cytotoxicity of cWBC may be related to apoptosis induction, as evidenced by typical apoptotic morphology features. Moreover, certain cWBC concentrations induced significant overproduction of ROS and significantly inhibited the S-G 2 /M transition in the cancer cell. The molecular mechanisms of cWBC in apoptosis induction were to decrease Bcl-2 and XIAP expression levels and increase the expression levels of caspase-3, caspase-8, and p53. These led to a decrease in the expression level of the cell cycle-associated gene cyclin-B1 and the arrest of cell population growth. Consequently, these findings demonstrate the prospect of the use of cWBC for cancer therapy.

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Anticancer Effect and Apoptosis Induction of Gambogic Acid in Human Leukemia Cell Line K562 In Vitro

Cited by 18 publications

References 28 publications

Inhibition of MicroRNA-149-5p Induces Apoptosis of Acute Myeloid Leukemia Cell Line THP-1 by Targeting Fas Ligand (FASLG)

Inhibition of MicroRNA-149-5p Induces Apoptosis of Acute Myeloid Leukemia Cell Line THP-1 by Targeting Fas Ligand (FASLG)

Gambogic Acid as Anticancer Agent: A Review

Siamese Crocodile White Blood Cell Extract Inhibits Cell Proliferation and Promotes Autophagy in Multiple Cancer Cell Lines

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