Anticancer compounds derived from fungal endophytes: their importance and future challenges

Kharwar, Ravindra N.; Mishra, Ashish; Gond, Surendra K.; Stierle, Andrea A.; Stierle, Donald B.

doi:10.1039/c1np00008j

Cited by 468 publications

(246 citation statements)

References 117 publications

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“…It is generally recognized that endophytes represent an important and largely untapped reservoir of unique chemical structures that have been modified through evolution (Gunatilaka 2006;Kharwar et al 2011) and exhibit the capability to produce the same functional compounds as their hosts, some examples include taxol (Stierle et al 1993(Stierle et al , 1995, podophyllotoxin , hypericin (Kusari et al 2008), and azadirachtin (Findlay et al 1997). In 2005, Puri et al reported the first discovery of CPT-producing endophytic fungus Entrophospora infrequens from the inner bark of N. foetida, which set the stage for a more comprehensive examination of other plants for the presence of CPT-producing endophytes Rehman et al 2008Rehman et al , 2009Kusari et al 2009;Shweta et al 2010Shweta et al , 2013Gurudatt et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Camptothecin-producing endophytic fungus Trichoderma atroviride LY357: isolation, identification, and fermentation conditions optimization for camptothecin production

Xiang

Xing

Chen

et al. 2013

Appl Microbiol Biotechnol

141

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Camptothecin (CPT), the third largest anticancer drug, is produced mainly by Camptotheca acuminata and Nothapodytes foetida. CPT itself is the starting material for clinical CPT-type drugs, but the plant-derived CPT cannot support the heavy demand from the global market. Research efforts have been made to identify novel sources for CPT. In this study, three CPT-producing endophytic fungi, Aspergillus sp. LY341, Aspergillus sp. LY355, and Trichoderma atroviride LY357, were isolated and identified from C. acuminata. Most CPT produced by these fungi was found in the fermentation broth, and their corresponding CPT yields were 7. 93, 42.92, and 197.82 μg l −1 , respectively. The CPT-producing capability of LY341 and LY355 was completely lost after repeat subculturing. A substantial decrease of CPT production was also observed in the second generation of LY357. However, a stable and sustainable production of CPT was found from the second generation through the eighth generation of LY357. The fermentation medium, time, pH, temperature, and agitation rate were optimized for CPT production. Methyl jasmonate and XAD16 were proven to be an optimum elicitor and adsorbent resin, respectively, in view of that CPT yield was increased 3.4-and 11-fold through their use. A 50-to 75-fold increase of CPT yield was obtained when the optimized fermentation conditions, elicitor, and adsorbent resin were combined and applied to the culture of the seventh and eighth generations of LY357, and the highest CPT yield was 142.15 μg l −1 . The CPTproducing T. atroviride LY357 paves a potential to uncover the mysteries of CPT biosynthesis.

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Section: Introductionmentioning

confidence: 99%

Camptothecin-producing endophytic fungus Trichoderma atroviride LY357: isolation, identification, and fermentation conditions optimization for camptothecin production

Xiang

Xing

Chen

et al. 2013

Appl Microbiol Biotechnol

141

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“…Plants may benefit indirectly from endophytes by increased resistance to herbivores, pathogen or stress or by other unknown mechanisms [24]. Many endophytes have the potential to synthesize various bioactive metabolites may, directly or indirectly, be used as therapeutic agents against numerous diseases [25]. Some studies have shown that endophytic fungi are able to protect their host plant from drought conditions [26].…”

Section: Endophytic Fungimentioning

confidence: 99%

Current Understanding of Endophytes: Their Relevance, Importance, and Industrial Potentials

Tidke¹,

Kl²,

Kiran³

et al. 2017

IOSR JBB

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“…As a result, 5.28 mg of 1, 1.23 mg of 2 and 4.32 mg of fusaric acid were prepared. The 1 H and 13 C NMR spectra were recorded on a JEOL ECX-500 spectrometer in DMSO-d 6 . The NMR data of 1 and 2 are summarized in Tables 1 and 2 …”

Section: Hplc Analysis Of the Compound Accumulated In Thementioning

confidence: 99%

“…[2][3][4][5] Endophytic fungi from plants produce numerous biologically active compounds. 6 These also include compounds reported as originating from plants, such as taxol and camptothecin. These reports suggested that gene transfer events may occur between endophytic fungi and plants.…”

Section: Introductionmentioning

confidence: 99%

Epigenetic modifier-induced biosynthesis of novel fusaric acid derivatives in endophytic fungi from Datura stramonium L.

Chen

Awakawa

Sun

et al. 2013

Nat. Prod. Bioprospect.

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Abstract:The treatment of fungi with DNA methyltransferase (DNMT) and/or histone deacetylase (HDAC) inhibitors is a promising way to activate secondary metabolite biosynthetic pathways that are dormant under normal conditions. In this study, we included an HDAC inhibitor, suberoylanilide hydroxamic acid (SBHA), in the culture medium of endophytic fungi isolated from the medicinal plant Datura stramonium L. The production of two compounds was induced in the culture supplemented with SBHA, and their structures were determined to be the fusaric acid derivatives 5-butyl-6-oxo-1,6-dihydropyridine-2-carboxylic acid and 5-(but-9-enyl)-6-oxo-1,6-dihydropyridine-2-carboxylic acid. The result confirmed that the use of chemical epigenetic modifiers is an effective technique for promoting the expression of silent biosynthetic pathways to produce unique secondary metabolites.

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Anticancer compounds derived from fungal endophytes: their importance and future challenges

Cited by 468 publications

References 117 publications

Camptothecin-producing endophytic fungus Trichoderma atroviride LY357: isolation, identification, and fermentation conditions optimization for camptothecin production

Camptothecin-producing endophytic fungus Trichoderma atroviride LY357: isolation, identification, and fermentation conditions optimization for camptothecin production

Current Understanding of Endophytes: Their Relevance, Importance, and Industrial Potentials

Epigenetic modifier-induced biosynthesis of novel fusaric acid derivatives in endophytic fungi from Datura stramonium L.

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