Anticancer, antithrombotic, antityrosinase, and anti‐α‐glucosidase activities of selected wild and commercial mushrooms from Pakistan

Abstract

Mushrooms have been accepted as nutraceutical foods because of their high nutritional and functional values. They have also gained interest due to their medicinal properties, economic importance, and organoleptic merit. In this study, wild Ganoderma lucidum and four commercial mushrooms, that is, Pleurotus ostreatus, Volvariella volvacea, Hericium erinaceus, and Lentinus edodes from Pakistan were screened for their biological activities such as anticancer, antityrosinase, anti‐α‐glucosidase, and antithrombotic activities from their methanol, ethanol, and water extracts. Enzyme inhibition assay showed that selected mushrooms are potent inhibitors with %age inhibition ranging from 19.00% to 80.91%, and 32.85% to 83.38% for tyrosinase and α‐glucosidase, respectively. The best tyrosinase inhibition was shown by P. ostreatus whereas L. edodes was found best as α‐glucosidase inhibitor. These mushrooms were tested against cancer cell lines including HT‐29 colon and H‐1299 lungs carcinoma cell lines. G. lucidum showed 29% and 24% viability of cells against HT‐29 and H‐1299 cell lines, respectively. This antiproliferative effect was in dose‐dependent manner, and the maximum inhibition was observed at 200 μg/ml. Mushrooms extracts were also found effective against clot lysis. The percentage of clot lysis was in the range of 27%–29%. The research would provide knowledge to the people of Pakistan about the importance of locally available commercial mushrooms and wild mushrooms for health improvement and prevention against different kinds of diseases.

Antiradical and functional properties of subcritical water extracts from edible mushrooms and from commercial counterparts

“…Sharif et al . (2018) determined in mushrooms aqueous extracts, IC 50 values against HT‐29 colon cancer cells around 100 μg mL −1 , whereas other mushroom extracts provided reduction of 20–40% in cell viability. Hu et al .…”

“…Antitumor activity of mushrooms and their extracts was also confirmed by other studies. Sharif et al (2018) determined in mushrooms aqueous extracts, IC 50 values against HT-29 colon cancer cells around 100 lg mL À1 , whereas other mushroom extracts provided reduction of 20-40% in cell viability. Hu et al (2018) reported lower IC 50 values for a phenolic-rich P. eryngii extract against HCT-116 cells, being 48.8 and 36.9 lg mL À1 at 48 and 72 h, respectively.…”

Online Survey for Patient Outcomes on Hericium Erinaceous Mushroom

“…The newest study conducted in Pakistan studied the anti-proliferative effect of H. Erinaceus and other commercial mushrooms on cell line model, it confirmed the Anticancer, antithrombotic, anti-tyrosinase, and anti-αglucosidase activities of this mushroom. 24 In 2018 A Chinese study reported that erinacine which extracted from H. Erinaceus can promote apoptosis and inhibited cell proliferation by inducing MPTP opening and promote the release of cyt-C which plays a role in inhibiting the progression of Hepatocellular Carcinoma. 25 Further research showed that the extracts of H. Erinaceus given orally, have proved a tumor-suppressing activity similar to that of 5-fluorouracil, in the treatment of gastrointestinal cancers.…”

Mantarlarin Tibbi̇ Özelli̇kleri̇ İle İlgi̇li̇ i̇n Vi̇tro, i̇n Vi̇vo Ve Kli̇ni̇k Değerlendi̇rmeler

“…Wang ve ark. [10], G. lucidum'dan izole edilen ve bir triterpenoit olan Ganoderik asit-A'in insan hepatosellüler karsinom hücre hatlarına etkisini belirlemek üzere, farklı zaman dilimlerinde (24, 48 ve 72 saat) ve farklı konsantrasyonlarda (50,100,150,200,250 ve 300 µmol/L) yaptıkları uygulama sonucunda, 48 saat-100 µmol/L dozunda Ganoderik asit-A uygulamasının ardından HepG2 ve SMMC7721 insan hepatosellüler karsinom hücre hatlarının gelişimini önemli boyutta engellendiğini bildirmişlerdir. Ayrıca 72 saat 75 µmol/Ldozunda Ganoderik asit-A maruziyetinin benzer etki göstererek iki hücrenin de gelişimini önemli derece engellediği rapor edilmiş ve elde edilen sonuçlara göre en ideal tedavinin 100 ve 75 µmol/L olduğu öne sürülmüştür.…”