Anticancer activity test of ethyl acetate extract of endophytic fungi isolated from soursop leaf ( Annona muricata L.)

Minarni,; Artika, I Made; Julistiono, Heddy; Bermawie, Nurliani; Riyanti, Eny Ida; Hasim, Hasim; Hasan, Akhmad Endang Zainal

doi:10.1016/j.apjtm.2017.06.004

Cited by 36 publications

(30 citation statements)

References 21 publications

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“…[5][6][7] Based on previous research conducted by Minami et al, soursop leaves extract is reported had an anticancer activity against breast cancer cells through induction of apoptotic process. 8 Another study reported that soursop leaves extracts were known to increase expression of p53 gene and lower hsp70 expression, resulted in activation of apoptosis process and stable homeostasis in Raji cells. 9 However, research about cytotoxicity of Annona muricata leaves originating from Indonesia against cervical cancer are still limited.…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity of Soursop Leaves (Annona muricata) against Cervical HeLa Cancer Cells

Qorina

Arsianti²,

Fithrotunnisa

et al. 2020

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Background: Cervical cancer is the cancer with highest prevalence and the leading cause of women death in Indonesia. Current treatments available for cervical cancer are chemotherapy, radiation, surgery, and nuclear therapy. Unfortunately, these treatments still have several limitations due to serious side effects, development of resistance, and very expensive price. Therefore, it is necessary to develop effective and low-cost therapy to treat cervical cancer. One of which is by utilizing natural sources available in Indonesia such as soursop (Annona muricata) leaves which has been used in folk medicine as a treatment for various diseases, including cancer. However, studies about its cytotoxicity against cervical cancer in Indonesia are still limited. Objective: The aim of this research is to analyze the potency of A.muricataleaves extracts originated from Indonesia as a novel alternative treatment for cervical cancer. Materials and method: A.muricata leaves obtained from Serpong, West Java, Indonesia were grounded and macerated in three different solvents with various polarity, namely ethanol (polar solvent), ethyl acetate (semipolar solvent) and hexane (non-polar solvent). Subsequently, the extracts were diluted into 8 various concentrations. Cytotoxicity of A.muricataleaves extracts against HeLa cervical cancer cells were determined by MTT assay and expressed by IC 50 value. Results: The results showed that three extracts of A.muricata have strong cytotoxicity against cervical HeLa cells. The highest cytotoxic activity was shown by etanol extract with an IC 50 of 35.51 µg/mL, followed by ethyl acetate (IC 50 : 5.91 µg/mL), and hexane (IC 50 : 8.39 µg/mL). Conclusion: A.muricata leaves extracts are potential to be developed as a novel alternative therapy for cervical cancer.

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Section: Introductionmentioning

confidence: 99%

Cytotoxicity of Soursop Leaves (Annona muricata) against Cervical HeLa Cancer Cells

Qorina

Arsianti²,

Fithrotunnisa

et al. 2020

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“…Fungal extracts including Saccharomyces cerevisiae and the endophytic fungus Annona muricata extracts have been demonstrated previously to possess anticancer activity in cancer cell lines in vitro. [14,17] The present study reports the anticancer activity of the oleaginous fungus, M. alpine, which is also having one of the most efficient ARA-producing capabilities. The extracts of M. alpina which has been previously reported to have multiple biological activity including antioxidant and antibacterial activity [18] showed a good inhibitory effect on the cervical cancer cell line HeLa, as well as on the adenocarcinoma cell line A549.…”

Section: Discussionmentioning

confidence: 90%

“…The concentrate was stored at −80°C for future experimentation. [14] Cytotoxicity analysis of the selected strain by MTT assay About 200 µL of the culture of suspension cell (cell density 20,000 cells per well) was seeded into a 96-well plate (ELISA plate) and was allowed to grow for about 12 h. After completion of incubation, appropriate concentrations of the test agents ranging from 0.1 to 1000 µL were added to the plates and incubated for 24 h at 37°C in a 5% CO 2 atmosphere. After completion of the incubation period, the ELISA plates were removed from the CO 2 incubator and spent media were washed with D-PBS.…”

Section: Preparation Of Fungal Extractmentioning

confidence: 99%

Untitled

Mishra¹

2018

AJP

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Aim: The aim is to understand any possible cytotoxic activity of Mortierella alpina extract against carcinoma cell lines and possible interaction with normal cell lines. Materials and Methods: The extract of M. alpina at varying concentrations was incubated with the normal Vero cell lines and also the carcinoma cell lines HeLa and A549, after which 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay was carried out to determine the viable cell. Results and Discussion: The cytotoxic activities of M. alpina extract were quite strong against both HeLa and A549 carcinoma cell lines with inhibitory concentration 50 values of 106 and 116 µg/mL, respectively. However, the cytotoxic concentration 50 value of the fungal extract against the normal Vero cell line was 2674 µg/mL, which shows the very low toxic activity of the fungal extract against the normal cell lines. Further, the therapeutic index of M. alpina against HeLa and A549 cell lines was found to be 25.22 and 23.02, respectively. Conclusion: The present investigation indicates the probable use of M. alpina extracts in the treatment of carcinoma.

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“…Paclitaxel, camptotecin, podophyllotoxin, vinblastine, and vincristine were detected in endophytes(21). Minarni et al, found that ethyl acetate extracts of some endophytic fungi isolated from soursop leaf (Annona muricata L.) had a significant cytotoxic activity against MCF 7 cells(22). Also, cytotoxicity of endophytic fungi associated with Bostrychia tenella was evaluated against HL-60 (human leukemia), HCT-8 (human colon carcinoma), and SF-295 (glioblastoma); three samples exhibited efficient cell growth inhibition (80% -100%) in all tested tumor cell lines(21).…”

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confidence: 99%

Bioactivity of Mycoendophytes Isolated from Medicinal Plants Growing in Different Geographical Egyptian Habitats

Osman

El‐Beih

Khatab

et al. 2020

Jundishapur J Nat Pharm Prod

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Fifty-four endophytic fungal species were isolated from 10 medicinal plants growing on different geographical Egyptian habitats; 48 of them were morphologically identified, at least to genus level and all belonged to ascomycetes. Ethanolic extracts of 49 isolates were screened for their antibacterial activities against three Gram-positive and three Gram-negative bacteria; they were also screened for their antifungal activities against Candida albicans and Fusarium solani. Twenty-six isolates showed antibacterial activities at least against one tested bacterial strain and seven isolates showed antibacterial activities against both Gram-negative and-positive bacteria. Moreover, cytotoxicity of these extracts were tested against one normal cell line MRC-5 and another cancer cell line MCF-7 using MTT assay. It was found that a group of seven isolates exerted a reduced toxicity towards the normal cell line, but had a higher toxic effect on the cancer cell line. These isolates represent a promising source for anticancer compounds with low cytotoxicity against the normal cell lines.

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Anticancer activity test of ethyl acetate extract of endophytic fungi isolated from soursop leaf ( Annona muricata L.)

Abstract: The endophytic fungi isolated from soursop leaf has the potential to be used as a source of anticancer agents.

Cited by 36 publications

References 21 publications

Cytotoxicity of Soursop Leaves (Annona muricata) against Cervical HeLa Cancer Cells

Cytotoxicity of Soursop Leaves (Annona muricata) against Cervical HeLa Cancer Cells

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Bioactivity of Mycoendophytes Isolated from Medicinal Plants Growing in Different Geographical Egyptian Habitats

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