Anticancer activity of silver and copper embedded chitin nanocomposites against human breast cancer (MCF-7) cells

Dhanasekaran, Solairaj; Rameshthangam, Palanivel; Arunachalam, Gnanapragasam

doi:10.1016/j.ijbiomac.2017.07.078

Cited by 58 publications

(24 citation statements)

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“…With nanoparticles activity being dependent of their physicochemical properties, namely size, shape, and surface, their toxicity toward cells is also dependent of these properties (Bera et al, 2014 ; Sun et al, 2014 ; Rajchakit and Sarojini, 2017 ). A strategy followed to deal with these problems has been the development of different types of nanoparticles, including polymeric nanoparticles, micelles, or liposomes, with the advantage of being possible to shape their properties to increase the efficacy in targeting or drug delivering (Xie et al, 2014 ; Bilal et al, 2017 ; Solairaj et al, 2017 ). With the objective of reducing toxicity without reducing NP activity, another adopted strategy was the incorporation or surface derivatization with different ligands, such as antibodies, small organic molecules, or proteins/peptides (Chen et al, 2012 ; Gao et al, 2017 ).…”

Section: Nanoparticles In Therapeuticsmentioning

confidence: 99%

“…The application of nanolipid systems (like liposomes or micelles) or polymeric NPs (chitosan based or conjugated with polyethylene glycol, PEG) had special success (Hann and Prentice, 2001 ; Allen and Cullis, 2013 ; Cosco et al, 2014 ; Paolino et al, 2017 ). These NPs have the advantage of being more biocompatible, with the effects toward healthy cells being reduced and having an improved targeted-oriented activity (Solairaj et al, 2017 ). Water et al developed poly(lactic-co-glycolic acid) nanoparticles (PLGaNPs) that were used as drug delivery system for plectasin, an antibiotic specific for airway Staphylococcus aureus infection (Water et al, 2015 ).…”

Section: Nanoparticles In Therapeuticsmentioning

confidence: 99%

“…Metal nanoparticles with gold or silver core have been tested and showed to have natural anticancer activity, either in vitro or in vivo against tumors and cancer cells (Shanmugasundaram et al, 2017 ; Shmarakov et al, 2017 ). Following the improvements on the development of nanoparticles, combinations of copper and chitosan were also tested, with observable anticancer activity and less toxic effects (Solairaj et al, 2017 ). DLS was used here not only as a mere characterization technique, but as a tool to identify metal structures with higher colloidal stability and better size distribution (Shmarakov et al, 2017 ).…”

Section: Nanoparticles In Therapeuticsmentioning

confidence: 99%

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Application of Light Scattering Techniques to Nanoparticle Characterization and Development

et al. 2018

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Over the years, the scientific importance of nanoparticles for biomedical applications has increased. The high stability and biocompatibility, together with the low toxicity of the nanoparticles developed lead to their use as targeted drug delivery systems, bioimaging systems, and biosensors. The wide range of nanoparticles size, from 10 nm to 1 μm, as well as their optical properties, allow them to be studied using microscopy and spectroscopy techniques. In order to be effectively used, the physicochemical properties of nanoparticle formulations need to be taken into account, namely, particle size, surface charge distribution, surface derivatization and/or loading capacity, and related interactions. These properties need to be optimized considering the final nanoparticle intended biodistribution and target. In this review, we cover light scattering based techniques, namely dynamic light scattering and zeta-potential, used for the physicochemical characterization of nanoparticles. Dynamic light scattering is used to measure nanoparticles size, but also to evaluate their stability over time in suspension, at different pH and temperature conditions. Zeta-potential is used to characterize nanoparticles surface charge, obtaining information about their stability and surface interaction with other molecules. In this review, we focus on nanoparticle characterization and application in infection, cancer and cardiovascular diseases.

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Section: Nanoparticles In Therapeuticsmentioning

confidence: 99%

Section: Nanoparticles In Therapeuticsmentioning

confidence: 99%

Section: Nanoparticles In Therapeuticsmentioning

confidence: 99%

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Application of Light Scattering Techniques to Nanoparticle Characterization and Development

et al. 2018

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“…Similarly, Cu-NP extensively studied for antimicrobial activity, and recently antiviral and anticancer activity of Cu-NP is also reported (Nagajyothi et al 2017;Yugandhar et al 2017). Synergistic effect of Silver and copper nanoparticles embedded in chitin has shown enhanced toxicity in MCF-7 cancer cells comparing to Ag-NP or Cu-NP; this suggests that the formation of the AgCu-NP alloy may be a potent anticancer agent for MCF-7 breast cancer (Solairaj et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Synthesis and analysis of silver–copper alloy nanoparticles of different ratios manifest anticancer activity in breast cancer cells

et al. 2020

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Background Breast cancer is therapeutically very challenging to treat as it has the main four known genetic alterations, which result in the existence of several phenotypes leading to the difference in the mode of therapy and with poor outcome. Metallic nanoparticles of silver or copper have been studied previously as anticancer agents in breast cancer and other types of cancers. However, the anticancer effect of silver–copper alloy nanoparticles (AgCu-NP) is not studied in breast cancer. In this study, we aim to synthesize silver nanoparticles (Ag-NP), or copper nanoparticles (Cu-NP), and AgCu-NP and evaluate their toxicity in breast cancer and healthy breast cells. Results We synthesized sodium citrate and mercapto-propionic acid (MPA-3) capped water-soluble metallic nanoparticles of Ag-NP or Cu-NP and an alloy of three different combinations of AgCu-NP. High-resolution transmission electron microscopy characterization of nanoparticles revealed the spherical shape nanoparticles of varied sizes, furthermore dynamic light scattering characterization was performed, which investigated the hydrodynamic size and stability in phosphate buffer solution. Energy-dispersive X-ray spectroscopy (EDS) measurements were obtained from the transmission electron microscope to study the composition of alloy nanoparticles and the distribution pattern of silver and copper in the alloy nanoparticles. We measured the toxicity of nanoparticles to breast cancer MCF-7 cell line by MTT assay and compared the toxic effect with non-cancerous breast epithelial cells MCF-10A. Our data showed that Ag-NP or Cu-NP have no effect on cancer cells or healthy cells, except Ag-NP at 20 µg/ml were toxic to cancer cells. However, AgCu-NP were significantly toxic to MCF-7 cells at 10 µg/ml concentration, while as AgCu-NP have no toxic effect on healthy cells. Furthermore, we observed the cell death pathway by the apoptosis marker Annexin-V which showed non-significant results, while the exposure of AgCu-NP in MCF-7 cells leads to toxicity and also caused significant increase in MMP-9 level, which suggests the cell death may be associated with other pathways such as autophagy and oxidative stress related. Conclusion The data suggest that the AgCu-NP alloy imposes preferential toxicity in breast cancer MCF-7 cells and thus could be exploited as a new candidate for further anticancer investigation

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“…Also, the impact of these nano particles has been approved in bone mineralization, the adhesion and proliferation of osteoblast cells, and finally, the synthesis of conjunctive tissue of bone (Forero, Roa, Reyes, Acevedo, & Osses, 2017; Hejazy et al, 2018). Moreover, anticancer properties have been also observed for copper metals (nitrate salts and nanoparticles; Bahojb Noruzi et al, 2019; Bahojb Noruzi et al, 2020; Solairaj, Rameshthangam, & Arunachalam, 2017; Yang et al, 2018). Despite their great potential and positive aspects in biomedical applications, it was reported that the induction of intense inflammatory responses and cellular toxicity is conducted by copper nanoparticles (Worthington et al, 2013).…”

Section: Applications Of Metal Nanomaterials For Bone Tissue Engineeringmentioning

confidence: 96%

Metal‐based nanoparticles for bone tissue engineering

Eivazzadeh‐Keihan

Noruzi

Chenab

et al. 2020

J Tissue Eng Regen Med

143

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Tissue is vital to the organization of multicellular organisms, because it creates the different organs and provides the main scaffold for body shape. The quest for effective methods to allow tissue regeneration and create scaffolds for new tissue growth has intensified in recent years. Tissue engineering has recently used some promising alternatives to existing conventional scaffold materials, many of which have been derived from nanotechnology. One important example of these is metal nanoparticles. The purpose of this review is to cover novel tissue engineering methods, paying special attention to those based on the use of metal‐based nanoparticles. The unique physiochemical properties of metal nanoparticles, such as antibacterial effects, shape memory phenomenon, low cytotoxicity, stimulation of the proliferation process, good mechanical and tensile strength, acceptable biocompatibility, significant osteogenic potential, and ability to regulate cell growth pathways, suggest that they can perform as novel types of scaffolds for bone tissue engineering. The basic principles of various nanoparticle‐based composites and scaffolds are discussed in this review. The merits and demerits of these particles are critically discussed, and their importance in bone tissue engineering is highlighted.

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Anticancer activity of silver and copper embedded chitin nanocomposites against human breast cancer (MCF-7) cells

Cited by 58 publications

References 59 publications

Application of Light Scattering Techniques to Nanoparticle Characterization and Development

Application of Light Scattering Techniques to Nanoparticle Characterization and Development

Synthesis and analysis of silver–copper alloy nanoparticles of different ratios manifest anticancer activity in breast cancer cells

Metal‐based nanoparticles for bone tissue engineering

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