Anticancer activity of fungal l-asparaginase conjugated with zinc oxide nanoparticles

Abstract

Demand for developing novel delivery system for cancer treatment has increased due to the side effects present in intravenous injection of L-asparaginase. Nanoparticles are used for delivering the drugs to its destination in cancer cure. Nanobiocomposite of zinc oxide nanoparticles conjugated with L-asparaginase was produced by Aspergillus terreus and was confirmed using maximum UV-Vis absorption at 340 nm in the present work. The presence of functional groups like OH, C-H, -C=N and C=O on the surface of nanobiocomposite was found from Fourier transform infrared spectrum analysis. Size of the produced nanocomposite was found in the range of 28-63 nm using scanning electron microscope. The crystalline nature of the synthesized nanobiocomposites was confirmed by X-ray diffraction analysis. The presence of zinc oxide on synthesized nanobiocomposite was confirmed by energy dispersive spectrum analysis. The anti-cancerous nature of the synthesized zinc oxide conjugated L-asparaginase nanobiocomposite on MCF-7 cell line was studied using MTT assay. The viability of the MCF-7 cells was decreased to 35.02 % when it was treated with L-asparaginase conjugated zinc oxide nanobiocomposite. Hence it is proved that the synthesized nanobiocomposites of zinc oxide conjugated L-asparaginase has good anti-cancerous activity.

Green synthesis of zinc oxide nanoparticles using Azadirachta indica A. Juss. leaves extract and its antibacterial activity against Xanthomonas orzyae pv. oryzae

“…In the present investigation, optical transitions have been observed for all the treatments which range from 332 -346 nm (Table 1). Similar absorbance spectra was observed in fungus mediated synthesis of zinc oxide nanoparticles (Baskar et al, 2013). It is clearly evident as depicted from the absorbance data that SPR absorbance tends to vary among different treatment combinations, owing to its sensitivity to nature, size and shape of the particles formed and also dependent upon particle to particle distance as well as its surrounding media (Kumar et al, 2014).…”

Nanomedicine in management of hepatocellular carcinoma: Challenges and opportunities

“…Moreover, these nanomaterials can be selectively delivered to the tumor sites via specific recognition of some molecules overexpressed by tumor tissues, and can also be equipped with diagnostic agents (fluorescent probes, metallic nanoparticles, radiolabels, etc .) for theranostic (therapy and diagnosis) applications …”

Biogenic Synthesis of Silver Chloride Nanoparticles from Padina gymnospora and their Evaluation of Antimicrobial, Larvicidal, Hemolytic and Anticancer Activities.

“…Nanotechnology plays a diverse role in medical sciences and pharmaceutical industries in recent years [1]. In connection with that, evolving nano-based therapeutics has received substantial attention over the last two decades [2][3][4][5].…”

“…In connection with that, evolving nano-based therapeutics has received substantial attention over the last two decades [2][3][4][5]. Researchers are tried to design the synthesis of nanoparticles for various applications which include therapeutics and diagnostics purposes, based on their cellular mechanisms and the advanced technology in nanomaterials [1]. Besides, silver-based nanoparticles have received more attention, due to their enhanced properties of physio-chemical and biological characteristics based upon the preparation of the nanoparticles by using additional elements [6].…”