Anticancer activity of flavonoids isolated from Achyrocline satureioides in gliomas cell lines

Souza, Priscila Oliveira de; Bianchi, S.; Figueiró, Fabrício; Heimfarth, Luana; Moresco, Karla Suzana; Gonçalves, Rosângela Mayer; Hoppe, Juliana Bender; Klein, Caroline; Salbego, Christianne Gazzana; Gelain, Daniel Pens; Bassani, Valquíria Linck; Zanotto-Filho, Alfeu; Moreira, José Cláudio Fonseca

doi:10.1016/j.tiv.2018.04.013

Cited by 45 publications

(29 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…11 In the field of glioma, Souza et al reported that flavonoids mixture could inhibit cell proliferation in glioma. 16 Wang et al indicated that luteolin inhibited glioma cell proliferation through endoplasmic reticulum stress and mitochondrial pathway. 17 Anson et al mentioned that luteolin decreased cell proliferation by inhibiting the epidermal growth factor signaling pathway in glioma.…”

mentioning

confidence: 99%

“…39 Although Souza et al found that luteolin could inhibit cell proliferation in glioma, the inherent mechanism had not been discussed. 16 Wang et al found that luteolin could induce apoptosis in glioma cells through ROS/ER stress and mitochondrial dysfunction. 17 Chakrabarti et al found that luteolin could stimulate cell apoptosis via regulating several apoptosis-related proteins and miR-7-1-3p upregulation in glioma.…”

mentioning

confidence: 99%

See 1 more Smart Citation

<p>Luteolin suppresses tumor proliferation through inducing apoptosis and autophagy via MAPK activation in glioma</p>

You¹,

Wang²,

Shao

et al. 2019

OTT

View full text Add to dashboard Cite

Purpose Glioma is a malignant tumor that originates in the brain and spine and is difficult to be completely removed. Though glioma patients receive active treatment, the survival rate is still poor. Therefore, it is urgent to discover a new medicine to treat glioma patients in order to improve the survival rate. In this study, we explored the anticancer effect and the potential mechanism of luteolin on glioma in vitro. Materials and methods Cell viability was determined by Cell Counting Kit-8 (CCK-8) assay. Fluorescent microscopy and flow cytometry analysis were used to determine the cellular apoptosis. Western blot analysis was performed to explore the changes in protein expression. Quantitative reverse transcription-PCR (qRT-PCR) analysis was utilized to evaluate the expression level of the tumor suppressor miR-124-3p. Results CCK-8 assays indicated that luteolin significantly inhibited glioma cell proliferation in a time- and dose-dependent manner. Fluorescent microscopy and flow cytometry analysis confirmed that luteolin induced glioma cell apoptosis. Western blot analysis showed that luteolin induced cellular apoptosis in glioma cells via MAPK activation (JNK, ERK, and p38). Luteolin stimulated the death receptor (FADD) to regulate the apoptosis proteins (Caspase-8, Caspase-3, and PARP). Luteolin increased the expression levels of LC3B II/I and downregulated the level of p62 that promotes cell autophagy. Finally, qRT-PCR confirmed that luteolin upregulated the expression levels of miR-124-3p. Conclusion These findings illustrate that luteolin may be a potential drug for glioma treatment.

show abstract

mentioning

confidence: 99%

mentioning

confidence: 99%

<p>Luteolin suppresses tumor proliferation through inducing apoptosis and autophagy via MAPK activation in glioma</p>

You¹,

Wang²,

Shao

et al. 2019

OTT

View full text Add to dashboard Cite

show abstract

“…Many pharmaceutical based businesses have identified the role of plants in sourcing for pharmacological compounds because they have immense medicinal properties [3]; secondary metabolites that are of great importance include flavonoids, tannins, alkaloids, phenols, saponins, terpenoids and cardiac glycosides are from plants [4]. Each of the secondary metabolites possess explicit function such as antifungal action of saponin [5], antimicrobial activity of tannin [6], usefulness of certain alkaloids for the treatment of HIV infection [7], potent anti-cancer activity of flavonoids [8]. Medicinal plants extensively occur globally and now it's getting more consideration because they have several beneficial properties to all human beings especially in pharmacological and medicine field.…”

Section: Phytochemicalsmentioning

confidence: 99%

Phytochemical Status of Different Solvent Extracts of Anthocleista djalonensis A. Chev Leaves

Popoola

2019

Int. Ann. Sci.

View full text Add to dashboard Cite

The choice of suitable solvent is essential in the extraction of much needed bioactive phytochemicals present in medicinal plants. This study assessed the phytochemical status of four solvents (methanol, hexane, ethylacetate and aqueous) extracts of Anthocleista djalonensis, leaves of the plants were extracted with the solvents by cold maceration for three days; screening and quantitative determination of saponin, alkaloid, tannin, cardiac glycosides, flavonoids phenols and terpenoids was done using standard methods. Phytochemical screening indicates the presence of all the phytocompound in the methanol extract, while other extracts shows variation in the availability of different phytochemicals, quantitative results shows alkaloid ranged from 1.79-18.90 mg/100g, flavonoids: 2.54-10.53 mg/100g, saponin: 2.00-12.23 mg/100g, tannin: ND-4.83 mg/100g, phenol: ND-6.65 mg/100g, cardiac glycoside: ND-2.08 mg/100g, and terpenoids: ND-1.98 mg/100g; significant difference (p<0.05) exist in each phytochemicals across the solvents where methanol and the aqueous extracts gave the best extraction efficiency for the phytochemicals. Consequently, the optimum extraction of useful phytochemicals from A. djalonensis could be achieved using either methanol and water.

show abstract

“…(2018) reported that the combination of flavonoids has synergistic anticancer activity (de Souza et al, 2018). It has potential for further research in vivo.…”

Section: Anticancer Activities Of the Flavonoidsmentioning

confidence: 99%

Antioxidant, antihypertensive, and anticancer activities of the flavonoid fractions from green, oolong, and black tea infusion waste

et al. 2018

View full text Add to dashboard Cite

The flavonoid fractions from green (GTF), oolong (OTF) and black tea (BTF) infusion waste were comparatively studied on the antioxidant, antihypertensive and anticancer activities. HPLC‐MS method was used to analyze the compositions of the flavonoid fractions. BTF exhibited the highest anti‐hypertension effect and anticancer activities. Quercetin‐3‐O‐rutinoside (QR) was highly related to angiotensin I‐converting enzyme (ACE) inhibitory effect (r = 1.000), and BTF exhibited the highest ACE inhibitory effect with the IC50 value of 46.72 µg/mL. BTF administration resulted in systolic decrease of 57.08 mmHg at 8 hr which was even higher than captopril (p < 0.05). The IC50 value of BTF on the growth of SMMC7721 cells was 52.31 µg/mL. Quercetin‐3‐O‐rutinoside, kaempferol 3‐O‐rutinoside, kaempferol 3‐O‐glucoside and patuletin O‐glucoside might be the effective constituents in BTF by the principal components analysis. BTF might be good nutrition supplements in the treatment of hypertension and hepatocellular carcinoma. Practical applications Tea is a kind of healthy and lowest cost beverages globally. It is generally consumed in three forms including green tea, oolong tea and black tea. Flavonoids can lower the blood pressure and inhibit the cancer cells growth due to their antioxidant activities. The flavonoids could produce undisputed antihypertensive effects and had the ability of preventing cancer that induced by oxidative stress. In addition, the flavonoids could be used as potential anticancer agent through inducing apoptosis and metastasis of the tumor cells. The relationships between the components of the flavonoids with antihypertensive and anticancer activities were comparatively investigated. The research could provide a new nutrition supplements in the treatment of hypertension and hepatocellular carcinoma.

show abstract

Anticancer activity of flavonoids isolated from Achyrocline satureioides in gliomas cell lines

Cited by 45 publications

References 42 publications

<p>Luteolin suppresses tumor proliferation through inducing apoptosis and autophagy via MAPK activation in glioma</p>

<p>Luteolin suppresses tumor proliferation through inducing apoptosis and autophagy via MAPK activation in glioma</p>

Phytochemical Status of Different Solvent Extracts of Anthocleista djalonensis A. Chev Leaves

Antioxidant, antihypertensive, and anticancer activities of the flavonoid fractions from green, oolong, and black tea infusion waste

Contact Info

Product

Resources

About