ANTICANCER ACTIVITY OF CALANONE ON HeLa CELL LINE

Ekowati, Heny; Astuti, Indwiani; Mustofa, Mustofa

doi:10.22146/ijc.21467

Cited by 20 publications

(10 citation statements)

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“…After 24 h, cells were treated with C. betacea extract (IC 50 values), doxorubicin (8 µg/ml) that served as positive control and culture medium (1% DMSO) that served as negative control for 24, 48 and 72 h. After exposure of grown cells in the extract, cells were washed with phosphate buffer saline (PBS) and harvested by centrifugation (2000 rpm for 5 min) and washed twice with PBS. The pellet (10 µl) were mixed with 5 µl (10 µg/ml) acridine orange and 5 µl (10 µg/ml) PI and observed under fluorescence microscope (Ekowati et al 2010). …”

Section: Methodsmentioning

confidence: 99%

Phenolics profile and anti-proliferative activity of Cyphomandra Betacea fruit in breast and liver cancer cells

et al. 2016

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Background Cyphomandra betacea (C. betacea) belongs to the Solanaceae family. This study was aimed to evaluate the anti-proliferative of C.betacea crude extract against selected cancer cell lines (breast and liver cancer) and to identify the polyphenolics profile (phenolic acids and flavonoids) of C. betacea fruits. Anti-proliferative effect of the extracts was examined using MTT (3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide) assay, followed by cell morphology analysis using acridine orange and propidium iodide double staining.ResultsThe phenolics profile was characterized using high-performance liquid chromatography (HPLC). C. betacea extract showed a high cytotoxic effect against liver and breast cancer cell lines with the IC50 value of 30 and 80 µg/ml, respectively. Phenolics profiling showed that C. betacea extract has a wide variety of polyphenolic compounds which are the responsible substances underlying the cytotoxic activity. The microscopic examination analysed by acridine orange and propidium iodide double staining showed that viable cells of the liver and breast cells were well rounded, large and intact green nuclei compared with the treated cells that characterized by apoptotic features (shrunken nuclei with a less quantity of cytoplasm).ConclusionThe present study demonstrated that the anti-proliferative properties of C. betacea fruits were partially attributed to the rich phenolics content. It supports the hypothesis that C. betacea fruits have potential as an effective agent in cancer therapy.

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Section: Methodsmentioning

confidence: 99%

Phenolics profile and anti-proliferative activity of Cyphomandra Betacea fruit in breast and liver cancer cells

et al. 2016

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“…While IFNα-2b expressed in P. pastoris inhibited 44% breast cancer (MCF) cell proliferation at 20 nmol/L [33]. The IC 50 value of 5-fluorouracil (5-FU) as a drug for colorectal cancer was reported at 129.29 µg/ml [34]. The result showed that a cytotoxicity of purified IFNα-2b was also comparable with the commercial drug.…”

Section: Anticancer Activity Of Recombinant Ifnα-2b Against Colorectamentioning

confidence: 99%

Heterologous Expression of Interferon α-2b in Lactococcus lactis and its Biological Activity against Colorectal Cancer Cells

Meilina¹,

Budiarti²,

Mustopa³

et al. 2021

Kor. J. Microbiol. Biotechnol

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Type I Interferons (IFNα) are known for their role as biological anticancer agents owing to their cell-apoptosis inducing properties. Development of an appropriate, cost-effective host expression system is crucial for meeting the increasing demand for proteins. Therefore, this study aims to develop codon-optimized IFNα-2b in L. lactis NZ3900. These cells express extracellular protein using the NICE system and Usp 45 signal peptide. To validate the mature form of the expressed protein, the recombinant IFNα-2b was screened in a human colorectal cancer cell line using the cytotoxicity assay. The IFNα-2b was successfully cloned into the pNZ8148 vector, thereby generating recombinant L. lactis pNZ8148-SP Usp45 -IFNα-2b. The computational analysis of codon-optimized IFNα-2b revealed no mutation and amino acid changes; additionally, the codon-optimized IFNα-2b showed 100% similarity with native human IFNα-2b, in the BLAST analysis. The partial size exclusion chromatography (SEC) of extracellular protein yielded a 19 kDa protein, which was further confirmed by its positive binding to anti-IFNα-2b in the western blot analysis. The crude protein and SEC-purified partial fraction showed IC 50 values of 33.22 μg/ml and 127.2 μg/ml, respectively, which indicated better activity than the metabolites of L. lactis NZ3900 (231.8 μg/ml). These values were also comparable with those of the regular anticancer drug tamoxifen (105.5 μg/ml). These results demonstrated L. lactis as a promising host system that functions by utilizing the pNZ8148 NICE system. Meanwhile, codonoptimized usage of the inserted gene increased the optimal protein expression levels, which could be beneficial for its large-scale production. Taken together, the recombinant L. lactis IFNα-2b is a potential alternative treatment for colorectal cancer. Furthermore, its activity was analyzed in the WiDr cell line, to assess its colorectal anticancer activities in vivo.

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“…Moreover, the biological evaluations of coumarins revealed that the engrossment of innumerable pathways via coumarins acts as anticancer agents. They target a number of pathways in cancer like as kinase inhibition, cell cycle arrest, heat shock protein (HSP90) inhibition, angiogenesis inhibition, monocarboxylate transporters inhibition, antimitotic activity, carbonic anhydrase inhibition, telomerase inhibition, aromatase inhibition, and sulfatase inhibition ( Ekowati et al, 2010 ; Li et al, 2010 ; Wang et al, 2011 ; Bhattarai et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Three-Component Synthesis of Some New Coumarin Derivatives as Anticancer Agents

et al. 2022

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A three-component reaction for the synthesis of novel 3-heteroaryl-coumarin utilizing acetylcoumarin synthon under ultrasonic irradiation was developed using chitosan-grafted poly(vinylpyridine) as an eco-friendly catalyst. The process is a simple, facile, efficient procedure for the preparation of compounds displaying a thiazole ring linked to coumarin moiety. Moreover, all the products were evaluated for their anticancer activities against HEPG2-1. The results revealed that three new compounds showed promising anticancer activities.

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ANTICANCER ACTIVITY OF CALANONE ON HeLa CELL LINE

Cited by 20 publications

References 0 publications

Phenolics profile and anti-proliferative activity of Cyphomandra Betacea fruit in breast and liver cancer cells

Phenolics profile and anti-proliferative activity of Cyphomandra Betacea fruit in breast and liver cancer cells

Heterologous Expression of Interferon α-2b in Lactococcus lactis and its Biological Activity against Colorectal Cancer Cells

Three-Component Synthesis of Some New Coumarin Derivatives as Anticancer Agents

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