Anticancer activity of bovine α‐lactalbumin treated with microbial transglutaminase

Abstract

This study investigated the effects of bovine α‐lactalbumin (α‐La) treated with microbial transglutaminase on human cancer cells, cell cultures and growth rate assays. The anticancer activity for 10 mg/mL of bovine α‐lactalbumin (α‐La) was measured as ∼90% in a human colorectal cancer cell line HCT 116. For the human bone cancer cell line SJSA‐1, α‐La hydrolysis resulted in higher cytotoxicity compared to untreated tumour cells. The formation of polymers of α‐La was suppressed by the addition of ethylenediaminetetraacetic acid, indicating that polymers of α‐La are promoted by metal ions such as Ca2+. The effect of α‐La on the morphology of SJSA‐1 cells was manifested as morphological changes compatible with apoptosis. Bovine milk α‐La with and without microbial transglutaminase is considered a valuable food ingredient and a nutraceutical for human health.

Nano Milk Protein-Mucilage Complexes: Characterization and Anticancer Effect

“…In this context, Jeong and Hong [33] observed that trypsin hydrolysates of αlactalbumin had a cytotoxic effect on human bone cancer SJSA-1, human colorectal cancer HCT 116, and human gastric cancer NCI-N87 cell lines. It was found that α-lactalbumin, interact with cell surface modulators and alter cell growth rate, intracellular calcium, and the calcium transport rate [34]. It is worth noting that bovine lactoferrin induced apoptosis in MCF7 cells in a dose-dependent manner [35].…”