Anticancer Activity of a scarcely investigated Red Sea Brown Alga Hormophysa cuneiformis against HL60, A549, HCT116 and B16 Cell Lines

Osman, Nehal A.H.K.; Siam, A. A.; El-Manawy, Islam M.; Jeon, You‐Jin

doi:10.21608/ejabf.2020.75087

Cited by 3 publications

(9 citation statements)

References 21 publications

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“…The IC 50 results indicated the highest anticancer activity for the extract against HepG-2. These results agreed with the findings of Osman et al (2020), who studied the anticancer activity of methanol extract of H. cuneiformis among six algal extracts. Osman et al (2020) found that H. cuneiformis had the highest anticancer activity against HL60, A549, and HCT116 cancer cells in a concentration-dependent manner.…”

Section: Extract Cytotoxicity Against Normal and Cancer Cellssupporting

confidence: 92%

“…The main goal for researchers in developing anticancer drugs is to seek more effective alternatives with fewer side effects. Marine macroalgae and their isolated compounds have been studied and reported for their anticancer effects (Biris-Dorhoi et al, 2020;Gupta and Abu-Ghannam, 2011;Gutiérrez-Rodríguez et al, 2018;Moga et al, 2021;Osman et al, 2020;Zorofchian Moghadamtousi et al, 2014). An MTT test was utilized in the current investigation to assess the cytotoxicity of H. cuneiformis on the viability percentage of healthy cells (Vero cell) and cancer cell lines (MCF-7, HepG-2, and HEP-2).…”

Section: Extract Cytotoxicity Against Normal and Cancer Cellsmentioning

confidence: 99%

“…Flavonoids, fatty acids, fucoidan, alginate, fucoxanthin, phenolics, and other compounds may have unique health-promoting properties that might be exploited in human healthcare applications, including microbes and cancer treatment (Mohamed and Saber, 2019). Several studies have reported marine algae and their compounds for their antimicrobial and anticancer activity (Biris-Dorhoi et al, 2020;Osman et al, 2019Osman et al, , 2020Moga et al, 2021;Teleb et al, 2022). Egypt's Red Sea is full of valuable marine algae that still need to be investigated for their bioactivity (Osman et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…Several studies have reported marine algae and their compounds for their antimicrobial and anticancer activity (Biris-Dorhoi et al, 2020;Osman et al, 2019Osman et al, , 2020Moga et al, 2021;Teleb et al, 2022). Egypt's Red Sea is full of valuable marine algae that still need to be investigated for their bioactivity (Osman et al, 2020). Hormophysa cuneiformis is an abundant brown marine alga that blooms on the coral reefs of the Red Sea, especially when extensive expanses of dead coral are present (Rashad and El-Chaghaby, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…Hormophysa cuneiformis is an abundant brown marine alga that blooms on the coral reefs of the Red Sea, especially when extensive expanses of dead coral are present (Rashad and El-Chaghaby, 2020). Yet, this alga is scarcely investigated for its bioactivity and chemical profiling (Mohamed and Saber, 2019;Osman et al, 2020;Rashad and El-Chaghaby, 2020). Therefore, this research aimed to look into the possible antimicrobial and anticancer properties of ethanol extract from the brown alga H. cuneiformis and having a chemical profile for its extract.…”

Section: Introductionmentioning

confidence: 99%

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Anticancer and antimicrobial evaluation of extract from brown algae Hormophysa cuneiformis

Osman,

Abd-Elazeem,

Al-Eisa

et al. 2023

J Appl Biomed

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Aim: We investigated the antimicrobial and anticancer properties of an ethanol crude extract of Red Sea brown alga (Hormophysa cuneiformis) from Egypt. Methods: Extraction was achieved by mixing 100 g of sample powder with absolute ethanol, incubating at 37 °C overnight in a shaking incubator, and then collecting the extract. The extract's antimicrobial activity was tested using a well diffusion assay against the tested pathogens (Escherichia coli, Bacillus subtilis, Staphylococcus aureus, and Candida albicans) in comparison to commercial antibiotics. Anticancer activity was assessed using MTT assay on MCF-7, HepG-2, and HEP-2 cell lines. The anticancer mechanism of action against the HepG-2 cell line was investigated using cell cycle analysis, Annexin V, and antioxidant enzymes, in addition to transmission electron microscopy.Results: GC-MS phytoconstituent profile of the extract was dominant with fatty acids. A broad antimicrobial effect against all the pathogenic isolates of E. coli, S. aureus, B. subtitles, and C. albicans was demonstrated, especially at the high concentration in comparison to commercial antibiotics. The extract could inhibit the growth of the tested cell lines. We observed the most significant effect on HepG-2 cells, and the concentration of the extract played a role in the level of inhibition (IC 50 of 44.6 ± 0.6 µg/ml). The extract had negligible effects on Vero normal cell lines at the lower concentration, with slight toxicity (90.8% viability) at the highest concentration (500 µg/ml). At this same concentration, the extract caused 80-92% inhibition of the cancer cell lines. The extract appears to have demonstrated promising effects on cancer cells. It induces programmed cell death (apoptosis), arrests the cell cycle, and affects the oxidative/antioxidant balance within the cells, potentially leading to the suppression or elimination of cancer cells. These findings are encouraging and may have implications for cancer treatment or further research in this area. More action of extract was seen against bacteria than fungi, with a wide antibacterial impact against all of the tested isolates, notably at the high concentration in comparison to conventional antibiotics. Conclusion: According to the findings, H. cuneiformis may be a valuable source of chemicals that are both antimicrobial and anticancer.

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Section: Extract Cytotoxicity Against Normal and Cancer Cellssupporting

confidence: 92%

Section: Extract Cytotoxicity Against Normal and Cancer Cellsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Anticancer and antimicrobial evaluation of extract from brown algae Hormophysa cuneiformis

Osman,

Abd-Elazeem,

Al-Eisa

et al. 2023

J Appl Biomed

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Antitumor immunity and therapeutic properties of marine seaweeds-derived extracts in the treatment of cancer

et al. 2022

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Marine seaweeds are important sources of drugs with several pharmacological characteristics. The present study aims to evaluate the antitumor and antitumor immunological potentials of the extracts from the brown alga Padinapavonica and the red alga Janiarubens, inhibiting the Egyptian marine coasts. Hep-G2 cell lines were used for assessment of the antitumor efficacy of Padinapavonica and Janiarubens extracts in vitro, while Ehrlich ascites carcinoma (EAC) cells were applied to gain more antitumor immunity and antitumor insights of P.pavonica and J.rubens extracts in vivo. In vitro antitumor potentials of P.pavonica and J.rubens extracts were analyzed against human liver cancer Hep-G2 cells by MTT and trypan blue exclusion assays. In vivo antitumor immunological potentials of P.pavonica and J.rubens extracts at low, high, and prophylactic doses were analyzed by blood counting and flow cytometry in mice challenged with Ehrlich ascites carcinoma (EAC) cells. In vitro results revealed that P.pavonica and J.rubens extracts caused significant decreases in the number and viability of Hep-G2 cells in a dose-dependent manner as compared to untreated Hep-G2 cells or Cisplatin®-treated Hep-G2 cells. In vivo findings showed that P.pavonica and J.rubens extracts at low, high, and prophylactic doses significantly reduced the number and viability of EAC tumor cells accompanied by increases in EAC apoptosis compared to naïve EAC mouse. Additionally, P.pavonica and J.rubens extracts at low and prophylactic doses remarkably increased both the total WBC count and the relative numbers of lymphocytes and decreased the relative numbers of neutrophils and monocytes. Flow cytometric analysis showed that P.pavonica and J.rubens extracts at the treatment and the prophylactic doses resulted in a significant increase in the phenotypic expressions of CD4+ T, CD8+ T, and CD335 cells compared to naïve EAC mouse. Overall, both extracts P.pavonica and J.rubens possess potential antitumor and antitumor immunological effects with less toxicity, opening new approaches for further studies of the chemical and biological mechanisms behind these effects.

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Cytotoxic Activity of Green Seaweed Halimeda tuna Methanolic Extract Against Lung Cancer Cells

Husni

Gazali

Nurjanah

et al. 2023

J. Multidiscip. Appl. Nat. Sci.

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Lung cancer is a malignant tumor that attacks the lungs generated by carcinogenic free radicals such as cigarette smoke. Seaweed contains bioactive compounds that have the potential to reduce cancer-causing free radicals. This study aimed to determine the phytochemical content and cytotoxic activity of Halimeda tuna seaweed extract against lung cancer cells (A549). The H. tuna sample was macerated using methanol for 24 h. Cytotoxic test of H. tuna crude extract used the MTT test against A549. The crude extract was phytochemically tested and analyzed using gas chromatography–mass spectrometry (GC-MS). The results showed that the H. tuna crude extract had cytotoxic activity against A549 with an IC50 value of 2771 µg/mL. The phytochemical test showed that H. tuna crude extract contained flavonoids and steroids. showed the presence of fatty acid compounds including palmitic acid, oleic acid, myristic acid, palmitoleic acid and stearic acid. Based on the results can be concluded that H. tuna extract had cytotoxic activity against A549 with low cytotoxicity to be used as a chemo-preventive agent.

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Anticancer Activity of a scarcely investigated Red Sea Brown Alga Hormophysa cuneiformis against HL60, A549, HCT116 and B16 Cell Lines

Cited by 3 publications

References 21 publications

Anticancer and antimicrobial evaluation of extract from brown algae Hormophysa cuneiformis

Anticancer and antimicrobial evaluation of extract from brown algae Hormophysa cuneiformis

Antitumor immunity and therapeutic properties of marine seaweeds-derived extracts in the treatment of cancer

Cytotoxic Activity of Green Seaweed Halimeda tuna Methanolic Extract Against Lung Cancer Cells

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