Anticancer Activity Evaluation of Kuanoniamines A and C Isolated from the Marine Sponge Oceanapia sagittaria, Collected from the Gulf of Thailand

Kijjoa, Anake

doi:10.3390/md20070002

Cited by 9 publications

(13 citation statements)

References 15 publications

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“…Marine sponges are known to produce toxins and other compounds to repel and deter predators [33,34]. These compounds of marine sponges are reported to be of the bioactive compounds which can be used in the treatment of many diseases [35][36][37][38][39].…”

Section: Discussionmentioning

confidence: 99%

Larvicidal, Ovicidal, and Repellent Activities of Marine Sponge Cliona celata (Grant) Extracts against Culex quinquefasciatus Say and Aedes aegypti L. (Diptera: Culicidae)

Reegan¹,

Kinsalin²,

Paulraj³

et al. 2013

ISRN Entomology

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Solvent extracts of marine spongeCliona celata(Grant) were screened for larvicidal, ovicidal, and repellent properties against the filarial vectorCulex quinquefasciatusSay and dengue vectorAedes aegyptiL. Larvicidal and ovicidal activities of hexane, ethyl acetate, and methanol extracts were tested in four different concentrations ranging as 62.5, 125, 250, and 500 ppm. Among the three solvent extracts ofC. celata, methanol extract showed the highest larvicidal activity at 500 ppm against both mosquito species. The LC50and LC90values ofC. celatamethanol extract were recorded as 95.63 and 242.16 ppm againstC. quinquefasciatuslarvae and 158.40 and 780.16 ppm againstA. aegyptilarvae, respectively. Ovicidal activity was high in methanol extract, in which 100% ovicidal activity was recorded inC. quinquefasciatus, and 72% ovicidal activity was recorded inA. aegyptiat 500 ppm. The hexane extract was found to be the most effective protectant against the adult mosquitoes of both species. The mean protection time recorded in hexane extract was up to 273 and 165 min at 5 mg/cm2dosage againstC. quinquefasciatusandA. aegypti, respectively. Considering these bioactivities,C. celatacould be used to obtain some novel pesticidal molecules.

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Section: Discussionmentioning

confidence: 99%

Larvicidal, Ovicidal, and Repellent Activities of Marine Sponge Cliona celata (Grant) Extracts against Culex quinquefasciatus Say and Aedes aegypti L. (Diptera: Culicidae)

Reegan¹,

Kinsalin²,

Paulraj³

et al. 2013

ISRN Entomology

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“…The half inhibitory concentration was caculated as 0.357±0.083 μM in our experiments, and we also compared the IC 50 of many other marine compounds treated adenocarcinoma cells. Antipathine A, extacted from China Sea had a cell toxicity effect on gastric carcinoma (SGC-7901) cells, and the IC 50 was 67.38 mg/ml (22); The IC 50 of hantupeptin on leukemic cells (MOLT-4) and breast carcinoma cells (MCF-7) were 32 and 4.0 mM, respectively (23); the IC 50 of kuanoniamine A and kuanoniamine C on MCF-7 cells were 0.12±0.07 μM and 0.81±0.11 μM, respectively (24). We concluded that SAD Figure 6.…”

Section: Discussionmentioning

confidence: 99%

“…Briefly, the cells (10000/well) were incubated in a 96-well plate. Caspase inhibitor Z-VAD-FMK (20 μM) was added 15 min before SAD (IC 50 ) in a final volume of 100 μl and incubated for different times (12,24,36, 48 h). One control was the absence of caspase inbitor and the other was absence of SAD and the caspase inhibitor.…”

Section: Caspase Inhibition Assaymentioning

confidence: 99%

The cell toxicity effect of secalonic acid D on GH3 cells and the related mechanisms

Zhu¹

2009

Oncol Rep

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Abstract.To investigate the anti-pituitary adenoma effect of secalonic acid D (SAD) extracting from marine microorganisms, we used MTT colorimetric method to evaluate the proliferation of GH3 cells treated by SAD, the time-and dose-dependent effects and the value of IC 50 were recorded. Hoechst staining, TUNEL and flow cytometry methods were used to analyze the apoptosis rate of GH3 cells treated by SAD. Western blotting, RT-PCR and caspase inhibitor (Z-VAD-FMK) were used to investigate the possible mechanism of SAD induced apoptosis and the expression of growth hormone (GH). The results showed that SAD has a time-and dose-dependent effect on GH3 cells and the cytotoxic effect was mainly through apoptosis. The mechanisms were partly through the activity of caspase family and also G1/S phase block. In addition, SAD also can suppress the expression levels of growth hormone in GH3 cells, however, the RT-PCR results showed that the mechanism was not through changing the expression of GH mRNA. We concluded that SAD may be a potential anti-pituitary tumor drug and further in vivo studies should be performed.

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“…Los poríferos son los organismos pluricelulares vivientes más antiguos, considerados animales primitivos, obteniendo su alimento a partir de la filtración (Hernández y Hernández, 2005;Montes y Lener, 2003). Las esponjas se encuentran protegidas por un sistema inmune altamente complejo, han existido durante millones de años; tiempo en el cual, las bacterias asociadas a las esponjas, adquirieron en común un metabolismo complejo (Kijjoa et al, 2007;Takahashi et al, 2007).…”

Section: Introductionunclassified

Bioactividad de las esponjas marinas Cliona varians y Cinachyrella kuekenthali provenientes de Isla Larga, Bahía de Mochima, Estado Sucre, Venezuela / Bioactivity of Cliona varians and Cinachyrella kuekenthali marine sponges from Isla Larga, Mochima Bay, Sucre state, Venezuela

Guevara¹,

Armaro²,

Fariñas³

et al. 2016

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Las esponjas marinas Cliona varians y Cinachyrella kuekenthali, fueron recolectadas en Isla Larga, Bahía de Mochima, estado Sucre, Venezuela, para su estudio sobre bioactividad. Se procedió a realizar extracciones con acetato de etilo (AcOEt) a cada esponja, y posteriormente un screening químico y las pruebas biológicas señaladas a continuación: antibacteriana, antifúngica y letalidad contra nauplios I del crustáceo Artemia salina. Para ambas esponjas se detectó la presencia de alcaloides, esteroles insaturados y cumarinas, y además taninos y metilencetonas para C. varians. Los bioensayos demostraron que los extractos poseen actividad letal o tóxica muy fuerte contra el crustáceo A. salina, con valores de CL50 inferiores a 103 μg/ml a 24 h de exposición de las larvas. Posterior al fraccionamiento cromatográfico de la fracción soluble en AcOEt de cada esponja, se observó que se magnificó el efecto tóxico de las subfracciones provenientes de C. varians, obteniéndose valores de CL50 por debajo de 4 μg/ml. Los extractos en AcOEt de dichas esponjas no presentaron actividad contra las bacterias y hongos ensayados; al fraccionar C. varians, se mantuvo este comportamiento; sin embargo, la subfracción CV1 presentó actividad antibacteriana leve contra Listeria monocitogenes. Las subfracciones de C. kuekenthali no mostraron actividad antifúngica; sin embargo, esta especie aumentó su actividad antibacteriana actuando contra Escherichia coli, Staphylococcus aureus, Salmonella enteristiti, Listeria monocytogenes, Bacillus cereus, Pseudomonas aeruginosa, Salmonella ephimurium y Enterococus fecalis. Estos resultados perfilan a dichas esponjas como una fuente promisoria en futuras investigaciones de fármacos con actividad antitumoral, especialmente C. varians. ABSTRACTThe marine sponges Cliona varians and Cinachyrella kuekenthali, were collected in Isla Larga, Mochima Bay, Sucre state, Venezuela, to study their bioactivity. Each sponge was extracted with ethyl acetate (EtOAc) and then a chemical screening and the following biological tests were performed: antibacterial, antifungal and lethality against nauplii I of Artemia salina crustacean. The presence of alkaloids, unsaturated sterols and coumarins was detected for both sponges, and also tannins and metilenketones for C. varians. Bioassays showed that extracts have very strong lethal or toxic activity against A. salina nauplii, with LC50 values less than 103 μg/ml at 24 h of larvae exposure. After chromatographic fractionation of the EtOAc soluble fraction from each sponge, the toxic effect of the C. varians subfractions was magnified, obtaining LC50 values below to 4 μg/ml. EtOAc extracts of these sponges showed no activity against fungi and bacteria used in this study; this behavior was maintained when fractioning C. varians; however, CV1 subfraction showed mild antibacterial activity against Listeria monocytogenes. The C. kuekenthali subfractions did not show antifungal activity, but showed antibacterial effect against Escherichia coli, Staphylococcus aureus, Salmonella enteristiti, Listeria monocytogenes, Bacillus cereus, Pseudomonas aeruginosa, Salmonella ephimurium and Enterococcus faecalis. These results suggest these sponges as a promising source for future investigations of drugs with antitumoral activity, especially C. varians.

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Anticancer Activity Evaluation of Kuanoniamines A and C Isolated from the Marine Sponge Oceanapia sagittaria, Collected from the Gulf of Thailand

Cited by 9 publications

References 15 publications

Larvicidal, Ovicidal, and Repellent Activities of Marine Sponge Cliona celata (Grant) Extracts against Culex quinquefasciatus Say and Aedes aegypti L. (Diptera: Culicidae)

Larvicidal, Ovicidal, and Repellent Activities of Marine Sponge Cliona celata (Grant) Extracts against Culex quinquefasciatus Say and Aedes aegypti L. (Diptera: Culicidae)

The cell toxicity effect of secalonic acid D on GH3 cells and the related mechanisms

Bioactividad de las esponjas marinas Cliona varians y Cinachyrella kuekenthali provenientes de Isla Larga, Bahía de Mochima, Estado Sucre, Venezuela / Bioactivity of Cliona varians and Cinachyrella kuekenthali marine sponges from Isla Larga, Mochima Bay, Sucre state, Venezuela

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