Anticancer Activity, Antioxidant Activity, and Phenolic and Flavonoids Content of WildTragopogon porrifoliusPlant Extracts

Al‐Rimawi, Fuad; Rishmawi, Suzi; Ariqat, Sharehan H.; Khalid, Mahmoud; Warad, Ismail; Salah, Zaidoun

doi:10.1155/2016/9612490

Cited by 54 publications

(21 citation statements)

References 18 publications

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“…Similar to several other plant phenols [ 57 ], the anticancer activity of 2HF seem to be associated with pleiotropic effects on multiple cancer signaling pathways that regulate cell growth and proliferation, epithelial-mesenchymal transition (EMT), apoptosis, and angiogenesis. The cellular concentration of E-cadherin was increased by 2HF exposure, which was paralleled by a decrease in vimentin and fibronectin, paradigmatic for inhibition of EMT [ 58 ].…”

Section: Discussionmentioning

confidence: 99%

Anticancer activity of 2’-hydroxyflavanone towards lung cancer

Awasthi

Singhal

et al. 2018

Oncotarget

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In previous studies, we found that 2'-hydroxyflavonone (2HF), a citrus flavonoid, inhibits the growth of renal cell carcinoma in a VHL-dependent manner. This was associated with the inhibition of glutathione S-transferases (GSTs), the first step enzyme of the mercapturic acid pathway that catalyzes formation of glutathione-electrophile conjugates (GS-E). We studied 2HF in small cell (SCLC) and non-small cell (NSCLC) lung cancer cell lines for sensitivity to 2HF antineoplastic activity and to determine the role of the GS-E transporter Rlip (Ral-interacting protein; RLIP76; RALBP1) in the mechanism of action of 2HF. Our results show that 2HF induced apoptosis in both histological types of lung cancer and inhibited proliferation and growth through suppression of CDK4, CCNB1, PIK3CA, AKT and RPS6KB1 (P70S6K) signaling. Increased E-cadherin and reduced fibronectin and vimentin indicated inhibition of epithelial-mesenchymal transition. Additionally, 2HF inhibited efflux of doxorubicin and increased its accumulation in the cells, but did not add to the transport inhibitory effect of anti-Rlip antibodies alone. Binding of Rlip to 2HF was evident from successful purification of Rlip by 2HF affinity chromatography. Consistent with increased drug accumulation, combined treatment with 1-chloro-2, 4-dinitrobenzene, reduced the GI50 of 2HF by an order of magnitude. Results of in-vivo nude mouse xenograft studies of SCLC and NSCLC, which showed that orally administered 2HF inhibited growth of both histological types of lung cancer, confirmed in-vitro study results. Our result suggest that Rlip inhibition is likely a mechanism of action. Our findings are basis of proposing 2HF as therapeutic or preventative drug for lung cancer.

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Section: Discussionmentioning

confidence: 99%

Anticancer activity of 2’-hydroxyflavanone towards lung cancer

Awasthi

Singhal

et al. 2018

Oncotarget

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“…Phenolic compounds are major sources of dietary antioxidants in the plants [ 21 ]. Antioxidants have beneficial health aspects, preventing and scavenging free radicals by means of donating hydrogen atoms to a free radical in order to protect biomolecules, such as proteins, lipids, carbohydrates and DNA [ 20 , 22 , 23 ], and to alleviate chronic diseases and degenerative ailments [ 24 ]. Thus, the beneficial effects of mistletoes may be attributed to their phenolic compounds [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

Comparative Evaluation of Soluble and Insoluble-Bound Phenolics and Antioxidant Activity of Two Chinese Mistletoes

Yang

et al. 2018

Molecules

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Mistletoes are used medicinally in order to treat various human illnesses. Few studies have reported on the phenolic content and antioxidant properties of Chinese mistletoes (CMs). In this work, the total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activities of soluble and insoluble-bound phenolic extracts from CMs hosted by Camellia assamica (Mast.) Chang (CMC) and Pyrus, i, f. (CMP) were compared. Phenolic compounds in CMC and CMP were identified and quantified using high-performance liquid chromatography (HPLC). The results indicated that the TPC of soluble phenolic extracts was higher than insoluble-bound phenolic counterparts in both CMC and CMP. In addition, the TPC of soluble, insoluble-bound and total phenolic fractions (9.91 ± 0.23, 4.59 ± 0.27 and 14.50 ± 0.35 μmol ferulic acid equivalents per gram (FAE/g) dry sample) extracted from CMP were higher than those extracted from CMC. The soluble phenolic extracts in CMP showed higher antioxidant activities than those in CMC. Eighteen phenolic compounds from soluble and insoluble-bound phenolic extracts from the CMs were identified and quantified by HPLC. This study indicates that CMC and CMP, especially the latter, could be sources of antioxidants in human health care.

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“…Substances capable of donating electrons reduce the colored radical solution to colorless neutral form which is evidenced by suppression of characteristic long wavelength absorption spectrum. The assay is widely used to evaluate antiradical activity of various kinds of specimens including plant extracts [13], [46], [52], [54], [56], [57]. In the present study, the leaf extract exhibited dose dependent scavenging of ABTS radicals with an IC 50 value of 6.03µg/ml.…”

Section: Resultsmentioning

confidence: 50%

Phytochemical analysis, Antimicrobial, insecticidal and antiradical activity of Hydnocarpus pentandra (Buch.-Ham.) Oken

Tr¹,

Kenie²,

Dm³

et al. 2017

ijpm

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Objectives: The present study was conducted to evaluate antimicrobial, insecticidal and radical scavenging activity of leaf extract of Hydnocarpus pentandra (Buch.-Ham.) Oken belonging to the family Achariaceae.Methods: Extraction process of shade dried and powdered leaf was carried out by maceration technique. Extract was screened for phytochemicals by standard tests. Antibacterial and antifungal activity of leaf extract was determined by Agar well diffusion and Poisoned food technique respectively. Antiradical activity of leaf extract was evaluated by two in vitro assays namely 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azinobis 3-ethylbenzothiazoline 6-sulfonate (ABTS) free radical scavenging assays. Insecticidal activity of leaf extract was determined against II instar and IV instar larvae of Aedes aegypti.Results: Preliminary phytochemical analysis showed the presence of alkaloids, flavonoids, tannins, saponins, glycosides, triterpenes and steroids in the leaf extract. Leaf extract exhibited marked inhibitory activity against Gram positive bacteria when compared to Gram negative bacteria. Bacillus cereus (zone of inhibition 1.86±0.05cm) and Escherichia coli (zone of inhibition 1.06±0.05cm) were inhibited to highest and least extent respectively. Extract was effective in inhibiting mycelial growth of seed-borne fungi. Among fungi, the susceptibility to extract was in the order: Curvularia sp. (53.64% inhibition) > Fusarium sp. (45.81% inhibition) > Alternaria sp. (35.08% inhibition). The extract exhibited concentration dependent larvicidal activity with marked activity being observed against II instar larvae (LC50 value 0.79mg/ml) when compared to IV instar larvae (LC50 value 1.37mg/ml). Leaf extract scavenged DPPH and ABTS radicals dose dependently with an IC50 value of 13.91µg/ml and 6.03µg/ml respectively.Conclusions: The plant is shown to be an important source of bioactive agents. The observed bioactivities could be attributed to the phytochemicals present in the leaf extract. Further studies on characterization and bioactivity determination of isolated components from leaf extract are to be carried out.

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Anticancer Activity, Antioxidant Activity, and Phenolic and Flavonoids Content of WildTragopogon porrifoliusPlant Extracts

Cited by 54 publications

References 18 publications

Anticancer activity of 2’-hydroxyflavanone towards lung cancer

Anticancer activity of 2’-hydroxyflavanone towards lung cancer

Comparative Evaluation of Soluble and Insoluble-Bound Phenolics and Antioxidant Activity of Two Chinese Mistletoes

Phytochemical analysis, Antimicrobial, insecticidal and antiradical activity of <em>Hydnocarpus pentandra</em> (Buch.-Ham.) Oken

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