Anticancer Activities of Surfactin and Potential Application of Nanotechnology Assisted Surfactin Delivery

Wu, Yuan Seng; Ngai, Siew Ching; Goh, Bey Hing; Chan, Kok‐Gan; Lee, Learn‐Han; Chuah, Lay Hong

doi:10.3389/fphar.2017.00761

Cited by 159 publications

(102 citation statements)

References 129 publications

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“…Surfactin, for example, forms ion-conducting channels in bacterial lipid membranes, akin to that of detergents 33 . Surfactin, as one of the most effective biosurfactants, is capable of destabilizing membranes that disrupt its integrity and permeability by forming pores 34 .…”

Section: Discussionmentioning

confidence: 99%

MBSP1: a biosurfactant protein derived from a metagenomic library with activity in oil degradation

Araújo

Silva-Portela

Lima

et al. 2020

Sci Rep

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Microorganisms represent the most abundant biomass on the planet; however, because of several cultivation technique limitations, most of this genetic patrimony has been inaccessible. Due to the advent of metagenomic methodologies, such limitations have been overcome. Prevailing over these limitations enabled the genetic pool of non-cultivable microorganisms to be exploited for improvements in the development of biotechnological products. By utilising a metagenomic approach, we identified a new gene related to biosurfactant production and hydrocarbon degradation. Environmental DNA was extracted from soil samples collected on the banks of the Jundiaí River (Natal, Brazil), and a metagenomic library was constructed. Functional screening identified the clone 3C6, which was positive for the biosurfactant protein and revealed an open reading frame (ORF) with high similarity to sequences encoding a hypothetical protein from species of the family Halobacteriaceae. This protein was purified and exhibited biosurfactant activity. Due to these properties, this protein was named metagenomic biosurfactant protein 1 (MBSP1). In addition, E. coli Rosetta tM (DE3) strain cells transformed with the MBSP1 clone showed an increase in aliphatic hydrocarbon degradation. In this study, we described a single gene encoding a protein with marked tensoactive properties that can be produced in a host cell, such as Escherichia coli, without substrate dependence. Furthermore, MBSP1 has been demonstrated as the first protein with these characteristics described in the Archaea or Bacteria domains.Surfactants are amphipathic compounds that have a hydrophobic moiety that is directed towards the surface and a hydrophilic portion that is directed towards the solution 1 . These amphiphilic molecules can reduce surface tension at air/water and oil/water interfaces 2,3 . Surfactants produced by organisms are called biosurfactants, which are extracellular products or components within the cell membranes of prokaryotes and eukaryotes 1,4,5 . Biosurfactants are classified into four major categories: glycolipids, fatty acids, lipopeptides, and polymeric types. These categories are represented by amphipathic polysaccharides, lipopolysaccharides, lipoproteins, fatty acids, or complex mixtures of these biopolymers. In general, the synthesis of biosurfactants involves elaborate genetic systems, including operons, non-ribosomal peptide synthetases, and/or multiprotein assembly complexes 6-9 .The synthesis of biosurfactants occurs in the presence of different substrates as a carbon source. To reduce production costs, cheaper substrates have been used. The most commonly used substrates for biosurfactant production are agro-industrial products such as molasses, marc, or vegetable oils 6-8 . Rhamnolipids and surfactin are among the best studied biosurfactants. Rhamnolipids are glycolipids first discovered in Pseudomonas aeruginosa, which are formed by the bonding between a rhamnose moiety and a 3-(3-hydroxyalkanoyloxy)alkanoic acid (HAA) fatty acid tail. The essen...

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Section: Discussionmentioning

confidence: 99%

MBSP1: a biosurfactant protein derived from a metagenomic library with activity in oil degradation

Araújo

Silva-Portela

Lima

et al. 2020

Sci Rep

View full text Add to dashboard Cite

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“…Antibacterial (Sabate, 2013) and antiviral (Jung et al, 2000) activity have been demonstrated. Its ability to lyze many types of cell membranes makes it a potential candidate for cancer treatment; however, to avoid lysis of noncancer cells, targeting cancer cell receptors is needed (Wu, 2017). Kabara (1972) found that fatty acids demonstrated efficacy against bacteria and yeast.…”

Section: Moorementioning

confidence: 99%

Surfactants as Antimicrobials: A Brief Overview of Microbial Interfacial Chemistry and Surfactant Antimicrobial Activity

Falk

2019

J Surfact & Detergents

130

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In this brief overview of a large and complex subject, as presented at the 2018 Surfactants in Solution conference, the need for, and impact of, hard surface antimicrobial products is demonstrated. The composition of the interfaces of three common classes of pathological microbes, bacteria, viruses, and fungi, is discussed so that surfactant and cleaning product development scientists better understand their interfacial characteristics. Studies of antimicrobial efficacy from the four major classes of surfactants (cationic, anionic, amphoteric, and nonionic) are shown. The need for preservatives in surfactants is elucidated. The regulatory aspects of antimicrobials in cleaning products to make antimicrobial claims are stressed.

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“…D2446) were also purchased from Sigma-Aldrich. PBS was selected as a vehicle based on solubility data from previous studies, which established that surfactin is soluble in PBS at levels well above the concentrations used here (57)(58)(59).…”

Section: Viruses Cells and In Vitro Infectionmentioning

confidence: 99%

Peptidoglycan-Associated Cyclic Lipopeptide Disrupts Viral Infectivity

Johnson

Hage

Kalveram

et al. 2019

J Virol

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Enteric viruses exploit bacterial components, including lipopolysaccharides (LPS) and peptidoglycan (PG), to facilitate infection in humans. Because of their origin in the bat enteric system, we wondered if severe acute respiratory syndrome coronavirus (SARS-CoV) or Middle East respiratory syndrome CoV (MERS-CoV) also use bacterial components to modulate infectivity. To test this question, we incubated CoVs with LPS and PG and evaluated infectivity, finding no change following LPS treatment. However, PG from Bacillus subtilis reduced infection Ͼ10,000-fold, while PG from other bacterial species failed to recapitulate this. Treatment with an alcohol solvent transferred inhibitory activity to the wash, and mass spectrometry revealed surfactin, a cyclic lipopeptide antibiotic, as the inhibitory compound. This antibiotic had robust dose-and temperature-dependent inhibition of CoV infectivity. Mechanistic studies indicated that surfactin disrupts CoV virion integrity, and surfactin treatment of the virus inoculum ablated infection in vivo. Finally, similar cyclic lipopeptides had no effect on CoV infectivity, and the inhibitory effect of surfactin extended broadly to enveloped viruses, including influenza, Ebola, Zika, Nipah, chikungunya, Una, Mayaro, Dugbe, and Crimean-Congo hemorrhagic fever viruses. Overall, our results indicate that peptidoglycan-associated surfactin has broad viricidal activity and suggest that bacteria by-products may negatively modulate virus infection. IMPORTANCE In this article, we consider a role for bacteria in shaping coronavirus infection. Taking cues from studies of enteric viruses, we initially investigated how bacterial surface components might improve CoV infection. Instead, we found that peptidoglycan-associated surfactin is a potent viricidal compound that disrupts virion integrity with broad activity against enveloped viruses. Our results indicate that interactions with commensal bacterial may improve or disrupt viral infections, highlighting the importance of understanding these microbial interactions and their implications for viral pathogenesis and treatment.

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Anticancer Activities of Surfactin and Potential Application of Nanotechnology Assisted Surfactin Delivery

Cited by 159 publications

References 129 publications

MBSP1: a biosurfactant protein derived from a metagenomic library with activity in oil degradation

MBSP1: a biosurfactant protein derived from a metagenomic library with activity in oil degradation

Surfactants as Antimicrobials: A Brief Overview of Microbial Interfacial Chemistry and Surfactant Antimicrobial Activity

Peptidoglycan-Associated Cyclic Lipopeptide Disrupts Viral Infectivity

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