Antibothropic Action of Camellia sinensis Extract Against the Neuromuscular Blockade by Bothrops jararacussu Snake Venom and Its Main Toxin, Bothropstoxin-I

Oshima-Franco, Yoko; Rosa, Luana de Jesus Reis; Silva, Gleidy Ana Araujo; Filho, Jorge Amaral; Silva, Magali Glauzer; Lopes, José Carlos Cogo Patricia Santos; Cintra, Adélia Cristina Oliveira; Cruz‐Höfling, Maria Alice da

doi:10.5772/33668

Cited by 5 publications

(8 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The pool of BthTX-I used in this work caused low myotoxicity when compared to other data shown in previous investigations, where 20 μg of toxin/mL induced 67 ± 2.3 % of damage cell in PND preparations. 33 Here, we have used 50 μg of toxin/mL to obtain complete muscle paralysis. Under pre- and post-toxin incubation protocols, VhE contributed to decrease in ~50 % the cell damage caused by BthTX-I.…”

Section: Resultsmentioning

confidence: 99%

Pharmacological Properties of Vochysia Haenkeana (Vochysiaceae) Extract to Neutralize the Neuromuscular Blockade Induced by Bothropstoxin-I (Lys49 Phospholipase A2) Myotoxin

et al. 2017

Self Cite

View full text Add to dashboard Cite

Purpose: Bothrops snakes are responsible for more than 70 % of snakebites every year in Brazil and their venoms cause severe local and systemic damages. The pharmacological properties of medicinal plants have been widely investigated in order to discover new alternative treatments for different classes of diseases including neglected tropical diseases as envenomation by snakebites. In this work, we have investigated the ability of Vochysia haenkeana stem barks extract (VhE) to neutralize the neuromuscular effects caused by Bothropstoxin-I (BthTX-I), the major phospholipase A2 (PLA2) myotoxin from B. jararacussu venom.Methods: The biological compounds of VhE were analysed under thin layer chromatography (TLC) and its neutralizing ability against BthTX-I was assessed through twitch-tension recordings and histological analysis in mouse phrenic nerve-diaphragm (PND) preparations. The antimicrobial activity of VhE was assessed against S. aureus, E. coli and P. aeruginosa strains. The aggregation activity of VhE was analysed under protein precipitation assay.Results: VhE showed the presence of phenolic compound visualized by blue trace under TLC. VhE abolished the neuromuscular blockade caused by BthTX-I applying the pre-toxin incubation treatment and partially neutralized the BthTX-I action under post-toxin incubation treatment; VhE contributed slightly to decrease the myotoxicity induced by BthTX-I. The neutralizing mechanism of VhE may be related to protein aggregation. VhE showed no antimicrobial activity.Conclusion: V. haenkeana extract which has no antimicrobial activity exhibited neutralizing ability against the neuromuscular blockade caused by BthTX-I and also contributed to decrease its myotoxicity. Protein aggregation involving phenolic compounds may be related in these protective effects.

show abstract

Section: Resultsmentioning

confidence: 99%

Pharmacological Properties of Vochysia Haenkeana (Vochysiaceae) Extract to Neutralize the Neuromuscular Blockade Induced by Bothropstoxin-I (Lys49 Phospholipase A2) Myotoxin

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…In the present study, B. jararacussu venom was chosen to test if a plant with anti-inflammatory properties also has antiophidian potential, because B. jararacussu venom causes inflammation at the bite site [34] and causes an irreversible neuromuscular blockade in vitro [7,35,36]. …”

Section: Resultsmentioning

confidence: 99%

Vellozia flavicans Mart. ex Schult. hydroalcoholic extract inhibits the neuromuscular blockade induced by Bothrops jararacussu venom

Tribuiani

Silva

Ferraz

et al. 2014

BMC Complement Altern Med

Self Cite

View full text Add to dashboard Cite

BackgroundSnakebite is a significant public health issue in tropical countries. In Brazil, some of the most common snake envenomations are from Bothrops. Bothrops bites trigger local and systemic effects including edema, pain, erythema, cyanosis, infections, and necrosis. Vellozia flavicans is a plant from the Brazilian “cerrado” (savanna) that is popularly used as an anti-inflammatory medicine. Since inflammation develops quickly after Bothrops bites, which can lead to infection, the aim of the present study was to observe possible anti-snake venom and antimicrobial activities of V. flavicans (Vf).MethodsThe chromatographic profile of the main constituents from the Vf leaf hydroalcoholic extract was obtained by thin-layer chromatography (TLC). The anti-snake venom activity was measured by Vf’s ability to neutralize the in vitro neuromuscular blockade caused by Bothrops jararacussu venom (Bjssu) in a mouse phrenic nerve-diaphragm model (PND). After a 20 min incubation, preparations of PND were added to Tyrode’s solution (control); Vf (0.2, 0.5, 1, and 2 mg/mL); 40 μg/mL Bjssu; pre-incubation for 30 min with Bjssu and 1 mg/mL Vf; and a Bjssu pretreated preparation (for 10 min) followed by 1 mg/mL Vf. Myographic recording was performed, and the contractile responses were recorded. The antimicrobial activity (minimum inhibitory concentration [MIC] and minimum bactericidal concentration [MBC]) was obtained for Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Enterococcus faecalis, using gentamicin and vancomycin as positive controls.ResultsTLC analysis yielded several compounds from Vf, such as flavonoids (quercetin) and phenolic acids (chlorogenic acid). Bjssu completely blocked the contractile responses of PND preparations, while Vf preserved 97% (±10%) of the contractile responses when incubated with Bjssu. In the PND pretreated with Bjssu, Vf was able to inhibit the neuromuscular blockade progress. MIC and MBC of Vf ranged from 2.5 to 5.0 mg/mL for P. aeruginosa and S. aureus strains, while no antimicrobial activity was observed for E. coli and E. faecalis.ConclusionsThe hydroalcoholic extract from Vf leaves was able to neutralize and decrease the in vitro neuromuscular blockade caused by Bjssu. However, it did not show significant antimicrobial activity against the tested bacteria.

show abstract

“…Bip. ex Baker [8,17], Dipteryx alata Vogel [18], Camellia sinensis L. [19,20], Hypericum brasiliense Choisy [21], Vellozia lavicans Mart. Ex Schult.…”

Section: Resultsmentioning

confidence: 99%

Effects of Vochysia haenkeana extract on the neuromuscular blockade induced by Bothrops jararaca venom on chick biventer cervicis preparation in vitro

Silva¹,

Tribuiani²,

Oliveira³

et al. 2017

J Plant Sci Phytopathol

View full text Add to dashboard Cite

SUMMARYVochysia haenkeana extract (Vh-E) was assessed against the neuromuscular blockade induced by Bothrops jararaca venom on chick biventer cervicis (BC) preparation. Pre-and post-venom incubation treatments (Pre-vit and Post-vit) were analysed here. Contractures ACh (110 μM) and KCl (20 mM) were evoked before and after addition of venom without stimulation. Vh-E (600 μg/mL) under Pre-vit was more effi cient to neutralize the neuromuscular blockade by venom (40 μg/mL) [72.5±4.6% (venom) vs. 45.2±14% (Vh-E) of blockade, p<0.05, n=4]. Vh-E (600 μg/mL) did not cause signifi cant changes under Post-vit [72.5±4.6% (venom) vs. 63.4±8.2% (Vh-E) of blockade, n=4]. The Pre-vit inhibited the blockade of the contracture to ACh (106±17% of response; n=4) while the Post-vit was able to attenuate the effect of the venom on this contracture (55±5% of response; n=4); related to those contractures to KCl both of treatments with Vh-E attenuated the blocker effect of the venom (62.5±7.7% and 55±5% of response for Pre-vit and Post-vit, respectively; n=4). In conclusion, Vh-E neutralizes partially the neuromuscular blockade in Pre-vit, an effect that can be related to preserved function of "extrinsic" post-synaptic receptors, by measured contractures in response to ACh. The myotoxicity of the venom was signifi cantly reduced by Vh-E in both, Pre-vit and Post-vit, by measured contractures in response to KCl.

show abstract

Antibothropic Action of Camellia sinensis Extract Against the Neuromuscular Blockade by Bothrops jararacussu Snake Venom and Its Main Toxin, Bothropstoxin-I

Cited by 5 publications

References 50 publications

Pharmacological Properties of Vochysia Haenkeana (Vochysiaceae) Extract to Neutralize the Neuromuscular Blockade Induced by Bothropstoxin-I (Lys49 Phospholipase A2) Myotoxin

Pharmacological Properties of Vochysia Haenkeana (Vochysiaceae) Extract to Neutralize the Neuromuscular Blockade Induced by Bothropstoxin-I (Lys49 Phospholipase A2) Myotoxin

Vellozia flavicans Mart. ex Schult. hydroalcoholic extract inhibits the neuromuscular blockade induced by Bothrops jararacussu venom

Effects of Vochysia haenkeana extract on the neuromuscular blockade induced by Bothrops jararaca venom on chick biventer cervicis preparation in vitro

Contact Info

Product

Resources

About