Antibody to fibroblast growth factor 23-peptide reduces excreta phosphorus of laying hens

Ren, Zhouzheng; Ebrahimi, Mohammad; Bütz, Daniel E.; Sand, Jordan M.; Zhang, Keying; Cook, Mark E.

doi:10.3382/ps/pew189

Cited by 27 publications

(18 citation statements)

References 38 publications

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“…Statistical significance was considered at P < 0.05 and trends at P < 0.1. According to our previous data (Ren et al, 2017c), seven laying hens per treatment would be sufficient to reflect the influences of dietary phosphorus levels on serum phosphorus concentrations (two means, 1.32 compared to 2.11 mmol/L, with two-sided significance of P < 0.05, with a pooled standard error of 0.50). As expected, in experiment 2, a statistical power of > 0.80 and a two-sided significance of P < 0.001 were obtained on serum phosphate concentrations by sampling seven laying hens from each experimental diet.…”

Section: Discussionmentioning

confidence: 99%

Phosphorus Restriction Changes the Expression of Fibroblast Growth Factor 23 and Its Receptors in Laying Hens

Ren

Yan

et al. 2020

Front. Physiol.

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Dietary phosphorus oversupply wastes non-renewable natural resources and raises environmental concerns in animal agriculture. We hypothesized that laying hens do not need large safety margins for dietary phosphorus because of the existence of fibroblast growth factor 23 (FGF23). In experiment 1, a total of 504 Hy-Line Brown laying hens (40-week-old) were randomly assigned to seven diets (for each diet, six replicates of 12 hens), containing 0.12, 0.17, 0.22, 0.27, 0.32, 0.37, and 0.42% non-phytate phosphorus, respectively, for 15 weeks. In experiment 2, a total of 14 Hy-Line Brown laying hens (40-week-old) were randomly assigned to two diets: (1) phosphorus restricted (n = 7) diet containing 0.14% non-phytate phosphorus, and (2) regular phosphorus (n = 7) diet containing 0.32% non-phytate phosphorus, for 21 days. Laying performance and egg quality were investigated in experiments 1 and 2. Phosphorus excretion and physiological changes were determined in experiment 2. It was found that dietary non-phytate phosphorus levels had no effects (P > 0.05) on laying performance and egg quality in either experiment. In experiment 2, laying hens fed 0.14% non-phytate phosphorus had decreased phosphorus excretion (by 52.6%, P < 0.001) when compared to those fed 0.32% non-phytate phosphorus. In response to the 0.14% non-phytate phosphorus diet, laying hens in experiment 2 exhibited: (1) suppressed calvaria mRNA expressions of FGF23 (by 57.8%, P < 0.001) and fibroblast growth factor receptor 1 (FGFR1, by 52.8%, P = 0.012), (2) decreased serum levels of FGF23 (by 41.7%, P = 0.011) and phosphorus (by 40.3%, P < 0.001), (3) decreased kidney mRNA expressions of FGFR1 (by 66.0%, P = 0.040) and FGFR4 (by 63.3%, P = 0.012) and decreased kidney protein expression of type 2a sodium-phosphorus co-transporter (NPt2a, by 51%, P = 0.025), (4) increased duodenum protein expression of NPt2b (by 45%, P = 0.032), and (5) increased excretion of calcium (by 22.9%, P ≤ 0.024). Collectively, decreasing dietary non-phytate phosphorus by up to 0.12% had no negative effects on egg-production performance but significantly decreased phosphorus excretion in laying hens. The laying hens adjusted to low-phosphorus diets by increasing intestinal NPt2b protein production, which was associated with decreased serum FGF23 concentration. Decreasing dietary non-phytate phosphorus is suggested to laying-hen nutritionists.

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Section: Discussionmentioning

confidence: 99%

Phosphorus Restriction Changes the Expression of Fibroblast Growth Factor 23 and Its Receptors in Laying Hens

Ren

Yan

et al. 2020

Front. Physiol.

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“…(2017) reported that the perfusions of 1,25-dihydroxy-cholecalciferol [ 1,25(OH) 2 D 3 ] or parathyroid hormone ( PTH ) fragment (1-34) increased intestinal expressions (both mRNA and protein levels) of type IIb sodium-phosphate cotransporter ( NaPi-IIb ) and thereby enhanced phosphorus absorption in broilers. A Wisconsin group found that antibodies to fibroblast growth factor 23 were effective in increasing renal phosphate resorption and decreasing phosphate requirements and excretions in laying hens and its progeny chicks ( Ren et al., 2017a , Ren et al., 2017b , Ren et al., 2017c ). In addition, phytase, the most widely used dietary exogenous enzyme in animal production ( Adeola, 2011 ), breaks down phytate-phosphate from plant feed ingredients and dramatically reduces the animals' requirement for inorganic phosphorus ( Fernández et al., 2019 ).…”

Section: Introductionmentioning

confidence: 99%

The adaptability of Hy-Line Brown laying hens to low-phosphorus diets supplemented with phytase

et al. 2020

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Body phosphorus homeostasis network allows laying hens to adapt to wide range of changes in dietary phosphorus levels. Phytase hydrolyzes phytate rendering phosphorus and reduces the laying hens' requirements for inorganic phosphate rock. Here, we demonstrate that there is no need to keep large safety margins in dietary phosphorus when hens are fed with phytase. Hy-Line Brown laying hens (n = 504) were randomly assigned to 7 treatments (6 replicates of 12 birds). A corn-soybean meal–based diet, with no inorganic phosphate rock, was formulated to contain 0.12% nonphytate phosphorus ( nPP ), 3.8% calcium, and 2,000 FTU/kg phytase. Inorganic phosphate rock (di-calcium phosphate) was supplemented into the basal diet to create 6 other diets containing 0.17, 0.22, 0.27, 0.32, 0.37, and 0.42% nPP. Levels of calcium carbonate and zeolite powder were adjusted to make sure all the 7 experimental diets contained the same nutrition levels (including calcium and phytase) except nPP. The diets were subjected to laying hens from 29 to 40 wk of age. As a result, when supplemented with 2,000 FTU/kg phytase, extra supplementation of inorganic phosphate rock had no effects ( P > 0.05) on serum phosphorus levels, serum calcium levels, laying performance (laying rate, egg weight, feed intake, feed-to-egg ratio, and unqualified egg rate), egg quality (shell thickness, shell strength, albumen height, yolk color, and Haugh unit), and tibia quality parameters (breaking strength and ash, calcium, and phosphorus contents). Extra supplementation of inorganic phosphate rock linearly increased ( P < 0.01) fecal phosphorus excretion and linearly decreased ( P = 0.032) the apparent metabolizability of dietary phosphorus. While serum hormones and intestine gene expressions were varied within treatments, no consistent changes were found. In conclusion, the supplementation of inorganic phosphate rock (provided 0.05–0.30% extra nPP) to phytase-containing basal diets (2,000 FTU/kg; nPP = 0.12%) provided limited benefits to egg production performance in laying hens from 29 to 40 wk of age. Further investigating the body phosphorus homeostasis would help to understand the nutritional and physiological reasonability of formulating low-phosphorus diets in the laying hen industry.

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“…It could also induce the decrease of 1.25(OH) 2 D 3 plasma levels by the up regulation of the 24 hydroxylase gene in the kidney 18 . As active immunization of hens against FGF23 increases P retention 19 , these observations highlight this factor as an important regulator of the Ca/P balance in laying hens.…”

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confidence: 71%

“…Further research is needed to test strategies to improve the management of old hens. A first strategy could selectively target circulating FGF23, for example by immunization as previously tested in younger hens 19 . A second strategy could rely upon a better adaptation of dietary P supply to the needs of old hens, saving this nonrenewable resource and limiting the stimulation of FGF23 production.…”

Section: Resultsmentioning

confidence: 99%

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Increased expression of fibroblast growth factor 23 is the signature of a deteriorated Ca/P balance in ageing laying hens

Gloux

Roy

Même

et al. 2020

Sci Rep

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The present study concerned the effect of ageing in laying hens, from 23 to 90 weeks of age, on the regulation of Ca metabolism related to the requirement for eggshell mineralization. Samples were collected from parathyroid gland (PG), liver, jejunum, medullary bone (MB) and kidney for a quantitative study of candidate gene expression. Although parathyroid hormone (PTH) gene expression in the PG did not vary with age, a stronger challenge to Ca homeostasis was suggested in aged hens. Indeed gene expression of Ca transporters , Vitamin D Receptor (VDR) in the jejunum, and that of transient receptor potential channel subfamily V member 5 (TRPV5) in the kidney decreased. This could exacerbate bone resorption and impair bone accretion, as attested by a higher expression of the Carbonic Anhydrase 2 (CA2) gene and a lower expression of collagen type I alpha 1 chain (COL1A1) in the MB. The increased expression of Fibroblast Growth Factor 23 (FGF23) in the MB likely contributed to the decreased plasma levels of 1.25(OH)2D3 and the altered expression of target genes under its regulation. Our data highlights the molecular mechanisms underlying the osteoporotic syndrome previously documented in aged laying hens, thus providing new perspectives for future interventions.

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Antibody to fibroblast growth factor 23-peptide reduces excreta phosphorus of laying hens

Cited by 27 publications

References 38 publications

Phosphorus Restriction Changes the Expression of Fibroblast Growth Factor 23 and Its Receptors in Laying Hens

Phosphorus Restriction Changes the Expression of Fibroblast Growth Factor 23 and Its Receptors in Laying Hens

The adaptability of Hy-Line Brown laying hens to low-phosphorus diets supplemented with phytase

Increased expression of fibroblast growth factor 23 is the signature of a deteriorated Ca/P balance in ageing laying hens

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