Antibody test for Legionella pneumophila detection

Párraga-Niño, Noemí; Quero, Sara; Uría, Naroa; Castillo-Fernandez, Oscar; Ezenarro, Josune Jimenez; Muñoz, F. Javier; Sabrià, Miquel; García-Núñez, Marian

doi:10.1016/j.diagmicrobio.2017.11.005

Cited by 6 publications

(2 citation statements)

References 14 publications

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“…The antibody was selected from a previous work [33] as a specific sensitive antibody for the detection of L. pneumophila. This study confirmed that the LP3IIG2 antibody was able to recognise almost all the serogroups of L. pneumophila and did not cross-react with other microbial species.…”

Section: Legionella Pneumophila Concentration and Antibody Reactionmentioning

confidence: 99%

Rapid Detection of Legionella pneumophila in Drinking Water, Based on Filter Immunoassay and Chronoamperometric Measurement

et al. 2020

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Legionella is a pathogenic bacterium, ubiquitous in freshwater environments and able to colonise man-made water systems from which it can be transmitted to humans during outbreaks. The prevention of such outbreaks requires a fast, low cost, automated and often portable detection system. In this work, we present a combination of sample concentration, immunoassay detection, and measurement by chronoamperometry. A nitrocellulose microfiltration membrane is used as support for both the water sample concentration and the Legionella immunodetection. The horseradish peroxidase enzymatic label of the antibodies permits using the redox substrate 3,3′,5,5′-Tetramethylbenzidine to generate current changes proportional to the bacterial concentration present in drinking water. Carbon screen-printed electrodes are employed in the chronoamperometric measurements. Our system reduces the detection time: from the 10 days required by the conventional culture-based methods, to 2–3 h, which could be crucial to avoid outbreaks. Additionally, the system shows a linear response (R2 value of 0.99), being able to detect a range of Legionella concentrations between 101 and 104 cfu·mL−1 with a detection limit (LoD) of 4 cfu·mL−1.

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Section: Legionella Pneumophila Concentration and Antibody Reactionmentioning

confidence: 99%

Rapid Detection of Legionella pneumophila in Drinking Water, Based on Filter Immunoassay and Chronoamperometric Measurement

et al. 2020

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“…L. pneumophila is the Gram-negative bacterium responsible for Legionnaires' disease, a severe respiratory ailment transmitted through contaminated water droplets [37][38][39]. The global increase in L. pneumophila epidemics emphasizes the need for advanced diagnostic methods [40,41], given the limitations of traditional methods such as culture-based testing and PCR [42][43][44]. Custom-made reactive DC magnetron sputtering equipment was used to prepare the nanoplasmonic thin films composed of Au and Au-Ag NPs dispersed in a TiO 2 dielectric matrix.…”

Section: Introductionmentioning

confidence: 99%

Critical Issues on the Surface Functionalization of Plasmonic Au-Ag/TiO2 Thin Films with Thiolated Oligonucleotide-Based Biorecognition Elements

Costa,

Pereira-Silva,

Sousa

et al. 2024

Biosensors

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This work reports on the surface functionalization of a nanomaterial supporting localized surface plasmon resonances (LSPRs) with (synthetic) thiolated oligonucleotide-based biorecognition elements, envisaging the development of selective LSPR-based DNA biosensors. The LSPR thin-film transducers are composed of noble metal nanoparticles (NPs) embedded in a TiO2 dielectric matrix, produced cost-effectively and sustainably by magnetron sputtering. The study focused on the immobilization kinetics of thiolated oligonucleotide probes as biorecognition elements, followed by the evaluation of hybridization events with the target probe. The interaction between the thiolated oligonucleotide probe and the transducer’s surface was assessed by monitoring the LSPR signal with successive additions of probe solution through a microfluidic device. The device was specifically designed and fabricated for this work and adapted to a high-resolution LSPR spectroscopy system with portable characteristics. Benefiting from the synergetic characteristics of Ag and Au in the form of bimetallic nanoparticles, the Au-Ag/TiO2 thin film proved to be more sensitive to thiolated oligonucleotide binding events. Despite the successful surface functionalization with the biorecognition element, the detection of complementary oligonucleotides revealed electrostatic repulsion and steric hindrance, which hindered hybridization with the target oligonucleotide. This study points to an effect that is still poorly described in the literature and affects the design of LSPR biosensors based on nanoplasmonic thin films.

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